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AB41432

Anti-PAK6 (phospho S165) antibody

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(1 Publication)

Rabbit Polyclonal PAK6 phospho S165 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human PAK6 pS165.

View Alternative Names

PAK5, PAK6, Serine/threonine-protein kinase PAK 6, PAK-5, p21-activated kinase 6, PAK-6

1 Images
Western blot - Anti-PAK6 (phospho S165) antibody (AB41432)
  • WB

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Western blot - Anti-PAK6 (phospho S165) antibody (AB41432)

Membrane was incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1 hour at room temperature.

All lanes:

Western blot - Anti-PAK6 (phospho S165) antibody (ab41432) at 1/1000 dilution

Lanes 1 and 5:

Human PAK6 recombinant protein phosphorylated by ERK2

Lanes 2 and 6:

Human PAK6 recombinant protein phosphorylated by ERK2; blot exposed to lambda phosphatase before probing

Lane 3:

Human PAK6 recombinant protein phosphorylated by ERK2 with non-specific peptide

Lane 4:

Human PAK6 recombinant protein phosphorylated by ERK2 with PAK6 N-terminal peptide (<a href='/en-us/products/primary-antibodies/pak6-antibody-ab41431'>ab41431</a> immunogen)

Lane 7:

Human PAK6 recombinant protein phosphorylated by ERK2 with phospho-S165-PAK6 peptide (ab41432 immunogen)

Lane 8:

Human PAK6 recombinant protein phosphorylated by ERK2 with non-specific phospho-serine peptide

Predicted band size: 75 kDa

Observed band size: 75 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human PAK6 pS165. The exact immunogen used to generate this antibody is proprietary information.

Q9NQU5

Specificity

Phospho-Ser165-specific. The antibody detects a 75 kDa band on SDS-PAGE immunoblots of human PAK6 recombinant protein phosphorylated with ERK2. This reactivity is specifically blocked by pre-incubation with phospho-Ser165-PAK6 peptide and also by treatment with lambda phosphatase.

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Alternative Products

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

Do not aliquot.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
This antibody was cross-adsorbed to unphosphorylated-Ser165-PAK6 peptide, then affinity purified using phospho-Ser165-PAK6 peptide (immunogen).
Storage buffer
Preservative: 0.05% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
Up to 6 months
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Serine/threonine protein kinase that plays a role in the regulation of gene transcription. The kinase activity is induced by various effectors including AR or MAP2K6/MAPKK6. Phosphorylates the DNA-binding domain of androgen receptor/AR and thereby inhibits AR-mediated transcription. Also inhibits ESR1-mediated transcription. May play a role in cytoskeleton regulation by interacting with IQGAP1. May protect cells from apoptosis through phosphorylation of BAD.
See full target information PAK6 pS165
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine (Cambridge, Mass.) 31:78 PubMed40000966

2025

A novel mechanism of FTO modulating the progression of endometriosis through mediating the m6A methylation of GEF-H1 in a YTHDF1-dependent manner.

Applications

Unspecified application

Species

Unspecified reactive species

Xin-Yu Ding,Hua-Ying Zhang,Jia-Hao Chen,Meng-Jie Yang,Zhi-Xiong Huang,Yi-Hong Lei,Qin-Kun Sun,Jian-Bin Bai,Dian-Chao Lin,Jian-Fa Lan,Lu-Lu Ren,Zheng-Yi Chen,Wei-Dong Zhou,Qiong-Hua Chen
View all publications
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Product promise

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