AB154827

Anti-Palladin antibody [EPR9460(B)]

RabMAb
Recombinant
What is this?

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(2 Publications)

Rabbit Recombinant Monoclonal Palladin antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 2 publications.

View Alternative Names

KIAA0992, CGI-151, PALLD, Palladin, SIH002, Sarcoma antigen NY-SAR-77

2 Images

Flow Cytometry (Intracellular) - Anti-Palladin antibody [EPR9460(B)] (AB154827)

Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-Palladin antibody [EPR9460(B)] (AB154827)

Intracellular flow cytometric analysis of permeabilized HeLa cells labeling Palladin with ab154827 at 1/10 dilution (red) compared to a rabbit IgG negative control (green).

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Western blot - Anti-Palladin antibody [EPR9460(B)] (AB154827)

All lanes:

Western blot - Anti-Palladin antibody [EPR9460(B)] (ab154827) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

293T cell lysate at 10 µg

Lane 3:

PC3 cell lysate at 10 µg

Lane 4:

Caco-2 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 151 kDa

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Carrier free

Anti-Palladin antibody [EPR9460(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9460(B)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Palladin is a cytoskeletal protein that plays an important role in maintaining cell structure and function. It is often referred to simply by its name and it has a molecular weight of approximately 90 to 140 kDa depending on its isoform. Palladin is expressed in a variety of tissues but shows a higher presence in cells such as fibroblasts smooth muscle cells and cardiac tissues. This protein interacts with numerous actin-associated proteins and contributes to the organization of the actin cytoskeleton by promoting actin polymerization.

Biological function summary

Palladin interacts with at least four actin-binding proteins to influence cellular processes such as motility and adhesion. Palladin often forms part of larger protein complexes that regulate the cytoskeleton. Through these interactions it contributes to the maintenance of cell shape and the mechanical stability of cells. Palladin’s role extends to influencing cell signaling pathways that regulate cell movement and structural integrity.

Pathways

Researchers connect palladin functions with cytoskeletal organization and cell signaling. It participates in pathways that involve the RhoA signaling pathway and the mechanotransduction pathway. Palladin interacts closely with proteins such as profilin and VASP synergistically influencing actin polymerization. These interactions are important for various cellular activities such as migration and morphogenesis.

Researchers have found palladin to be linked with conditions such as cancer and certain fibrosis. Altered expression levels of palladin can lead to enhanced metastatic potential in cancer cells. Additionally it exhibits associations with BRCA1 in breast cancer where mutations can alter cytoskeletal dynamics thereby affecting cell proliferation and migration. Palladin's overexpression is also observed in pancreatic cancer connecting it to actin-associated proteins that influence disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Cytoskeletal protein required for organization of normal actin cytoskeleton. Roles in establishing cell morphology, motility, cell adhesion and cell-extracellular matrix interactions in a variety of cell types. May function as a scaffolding molecule with the potential to influence both actin polymerization and the assembly of existing actin filaments into higher-order arrays. Binds to proteins that bind to either monomeric or filamentous actin. Localizes at sites where active actin remodeling takes place, such as lamellipodia and membrane ruffles. Different isoforms may have functional differences. Involved in the control of morphological and cytoskeletal changes associated with dendritic cell maturation. Involved in targeting ACTN to specific subcellular foci.

See full target information PALLD

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Stem cells and development 30:725-735 PubMed33926240

2021

CD271CD51PALLADIN Human Mesenchymal Stromal Cells Possess Enhanced Ossicle-Forming Potential.

Applications

Unspecified application

Species

Unspecified reactive species

Maiko Sezaki,Subinoy Biswas,Sayuri Nakata,Motohiko Oshima,Shuhei Koide,Nicole Pui Yu Ho,Nobukazu Okamoto,Takeshi Miyamoto,Atsushi Iwama,Hitoshi Takizawa

eLife 4: PubMed25738228

2015

A striatal-enriched intronic GPCR modulates huntingtin levels and toxicity.

Applications

Unspecified application

Species

Unspecified reactive species

Yuwei Yao,Xiaotian Cui,Ismael Al-Ramahi,Xiaoli Sun,Bo Li,Jiapeng Hou,Marian Difiglia,James Palacino,Zhi-Ying Wu,Lixiang Ma,Juan Botas,Boxun Lu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Palladin antibody [EPR9460(B)]

Flow Cytometry (Intracellular) - Anti-Palladin antibody [EPR9460(B)] (AB154827)

Western blot - Anti-Palladin antibody [EPR9460(B)] (AB154827)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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