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AB249133

Anti-Palladin antibody [EPR9460(B)] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal Palladin antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

KIAA0992, CGI-151, PALLD, Palladin, SIH002, Sarcoma antigen NY-SAR-77

1 Images
Western blot - Anti-Palladin antibody [EPR9460(B)] - BSA and Azide free (AB249133)
  • WB

Unknown

Western blot - Anti-Palladin antibody [EPR9460(B)] - BSA and Azide free (AB249133)

This data was developed using ab154827, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Palladin antibody [EPR9460(B)] (<a href='/en-us/products/primary-antibodies/palladin-antibody-epr9460b-ab154827'>ab154827</a>) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

293T cell lysate at 10 µg

Lane 3:

PC3 cell lysate at 10 µg

Lane 4:

Caco-2 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 151 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9460(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab249133 is the carrier-free version of ab154827.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Palladin is a cytoskeletal protein that plays an important role in maintaining cell structure and function. It is often referred to simply by its name and it has a molecular weight of approximately 90 to 140 kDa depending on its isoform. Palladin is expressed in a variety of tissues but shows a higher presence in cells such as fibroblasts smooth muscle cells and cardiac tissues. This protein interacts with numerous actin-associated proteins and contributes to the organization of the actin cytoskeleton by promoting actin polymerization.
Biological function summary

Palladin interacts with at least four actin-binding proteins to influence cellular processes such as motility and adhesion. Palladin often forms part of larger protein complexes that regulate the cytoskeleton. Through these interactions it contributes to the maintenance of cell shape and the mechanical stability of cells. Palladin’s role extends to influencing cell signaling pathways that regulate cell movement and structural integrity.

Pathways

Researchers connect palladin functions with cytoskeletal organization and cell signaling. It participates in pathways that involve the RhoA signaling pathway and the mechanotransduction pathway. Palladin interacts closely with proteins such as profilin and VASP synergistically influencing actin polymerization. These interactions are important for various cellular activities such as migration and morphogenesis.

Researchers have found palladin to be linked with conditions such as cancer and certain fibrosis. Altered expression levels of palladin can lead to enhanced metastatic potential in cancer cells. Additionally it exhibits associations with BRCA1 in breast cancer where mutations can alter cytoskeletal dynamics thereby affecting cell proliferation and migration. Palladin's overexpression is also observed in pancreatic cancer connecting it to actin-associated proteins that influence disease progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cytoskeletal protein required for organization of normal actin cytoskeleton. Roles in establishing cell morphology, motility, cell adhesion and cell-extracellular matrix interactions in a variety of cell types. May function as a scaffolding molecule with the potential to influence both actin polymerization and the assembly of existing actin filaments into higher-order arrays. Binds to proteins that bind to either monomeric or filamentous actin. Localizes at sites where active actin remodeling takes place, such as lamellipodia and membrane ruffles. Different isoforms may have functional differences. Involved in the control of morphological and cytoskeletal changes associated with dendritic cell maturation. Involved in targeting ACTN to specific subcellular foci.
See full target information PALLD
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Developmental cell 60:2744-2760.e9 PubMed40460835

2025

ATG9A and ARFIP2 cooperate to control PI4P levels for lysosomal repair.

Applications

Unspecified application

Species

Unspecified reactive species

Stefano De Tito,Eugenia Almacellas,Daniel Dai Yu,Emily Millard,Wenxin Zhang,Cecilia de Heus,Christophe Queval,Javier H Hervás,Enrica Pellegrino,Ioanna Panagi,Ditte Fogde,Teresa L M Thurston,Judith Klumperman,Maximiliano Gutierrez,Sharon A Tooze
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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