Mouse Monoclonal alpha smooth muscle Actin antibody. Suitable for Flow Cyt, ELISA, WB, IHC-P and reacts with Human, Rat, Mouse, African green monkey, Recombinant fragment - Human samples. Cited in 28 publications. Immunogen corresponding to Synthetic Peptide within Human ACTA2 aa 350 to C-terminus conjugated to Keyhole Limpet Haemocyanin.
IgG1
Mouse
Preservative: 0.05% Sodium azide
Constituents: PBS
Liquid
Monoclonal
Flow Cyt | ELISA | WB | IHC-P | ICC/IF | |
---|---|---|---|---|---|
Human | Tested | Expected | Tested | Tested | Not recommended |
Mouse | Expected | Expected | Tested | Expected | Not recommended |
Rat | Expected | Expected | Tested | Expected | Not recommended |
African green monkey | Expected | Expected | Tested | Expected | Not recommended |
Recombinant fragment - Human | Not recommended | Tested | Not recommended | Not recommended | Not recommended |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/200.00000 - 1/400.00000 | Notes ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Species | Dilution info | Notes |
---|---|---|
Species Rat, Mouse, African green monkey | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Recombinant fragment - Human | Dilution info 1/10000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human, Rat, Mouse, African green monkey | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info 1/500.00000 - 1/2000.00000 | Notes - |
Species Mouse | Dilution info 1/500.00000 - 1/2000.00000 | Notes - |
Species African green monkey | Dilution info 1/500.00000 - 1/2000.00000 | Notes - |
Species Human | Dilution info 1/500.00000 - 1/2000.00000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/200.00000 - 1/1000.00000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat, Mouse, African green monkey | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, African green monkey, Human, Recombinant fragment - Human | Dilution info - | Notes - |
Select an associated product type
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
ACTSA, ACTVS, GIG46, ACTA2, Alpha-actin-2, Cell growth-inhibiting gene 46 protein
Mouse Monoclonal alpha smooth muscle Actin antibody. Suitable for Flow Cyt, ELISA, WB, IHC-P and reacts with Human, Rat, Mouse, African green monkey, Recombinant fragment - Human samples. Cited in 28 publications. Immunogen corresponding to Synthetic Peptide within Human ACTA2 aa 350 to C-terminus conjugated to Keyhole Limpet Haemocyanin.
IgG1
Mouse
Preservative: 0.05% Sodium azide
Constituents: PBS
Liquid
Monoclonal
4A4
Affinity purification Protein G
Actin proteins have very high homology. The immunogen for this product is based on the ACTA2 protein but this product will also detect Gamma actin, and likely other actin family members too.
Purified from tissue culture supernatant.
Blue Ice
1-2 weeks
+4°C
-20°C
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This supplementary information is collated from multiple sources and compiled automatically.
The actin protein sometimes referred to as "pan actin" is an important component of the cytoskeleton and is fundamental in maintaining cell structure and integrity. Alternately called beta-actin or gamma-actin depending which variant is expressed this protein has a molecular weight of approximately 42 kDa. Actin is ubiquitously expressed in almost all eukaryotic cells where it forms dynamic networks that support the plasma membrane assist in cell movement and mediate intracellular transport.
Actin polymerizes to form filaments (F-actin) from its monomeric forms (G-actin) within the cell. These filaments are critical for constructing the cytoskeleton and anchor cellular structures in place. Actin functions within the actin-myosin complex which facilitates muscle contraction and movement in non-muscle cells. Through this complex actin provides essential support in cellular processes such as cytokinesis and vesicle movement.
Actin is important for pathways that control cell motility and structural rearrangements. It plays a significant role in the Rho-GTPase signaling pathway which regulates actin cytoskeleton dynamics. Actin interacts with other important proteins such as myosin and tropomyosin to facilitate these activities. Another important pathway involving actin is the Wnt signaling pathway where it aids in the regulation of cell proliferation and differentiation.
Actin is linked to conditions such as cancer metastasis and muscular dystrophies. In cancer actin's role in cell motility and invasion contributes to the metastatic spread of tumors. Actin dysregulation is also implicated in certain muscle disorders where mutations affect the formation and function of muscle fibers. In these contexts actin closely connects with dystrophin an important protein in muscle tissue integrity and any disruption can lead to disease manifestation.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 ug per mL of recombinant protein Recombinant Human Alpha Skeletal Muscle Actin protein ab157838 and Recombinant Human gamma Actin protein ab157841 were immobilised in PBS on an ELISA plate overnight. After blocking in 5% BSA, primary antibody ab119952 was added in a concentration range of 0.017-1000 ng per mL.
Pre-absorbed secondary antibody goat anti-rabbit IgG H&L (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) was used at 1/20000 dilution.
Immunohistochemical analysis of paraffin-embedded Human duodenum tissue using ab119952 at a dilution of 1/200 with DAB staining.
Flow cytometric analysis of Hela cells using ab119952 at a dilution of 1/200 (green) and negative control (purple).
All lanes: Western blot - Anti-pan Actin Antibody [4A4] (ab119952) at 1/500 dilution
Lane 1: HeLa cell lysate
Lane 2: A431 cell lysate
Lane 3: Jurkat cell lysate
Lane 4: K562 cell lysate
Lane 5: HEK293 cell lysate
Lane 6: HepG2 cell lysate
Lane 7: NIH3T3 cell lysate
Lane 8: PC12 cell lysate
Lane 9: COS-7 (African green monkey kidney fibroblast-like cell line) cell lysate
Predicted band size: 42 kDa
Observed band size: 42 kDa
Immunohistochemical analysis of paraffin-embedded Human esophagus tissue using ab119952 at a dilution of 1/200 with DAB staining.
Image collected and cropped by CiteAb under a CC-BY license from the publication
pan Actin western blot using anti-pan Actin Antibody [4A4] ab119952. Publication image and figure legend from Su, M., Fan, S., et al., 2020, Front Physiol, PubMed 32733269.
ab119952 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab119952 please see the product overview.
MiR-223 deficiency in diabetic platelet fails to suppress VSMC proliferation. Activated platelets (APs) from healthy subject (HS) and DM patients were cocultured with human VSMCs for 48 h. (A) Expression of differentiation markers ACTA2, CNN1, and TAGLN and dedifferentiation markers OPN, MYH10, KLF4, and KLF5 in control (Ctrl) VSMCs and APs cocultured with VSMCs (HS-APs, DM-APs) was detected with western blot analysis. (B,C) The rate of proliferation was determined by CCK8 assay and Brdu assay in VSMCs treated with APs pretreated by RNase A (degrades the RNA). Data are presented as mean ± SD (n = 6). Comparisons between more than two groups were analyzed using one-way ANOVA analysis with the Dunnett’s multiple correction. *P < 0.05, **P < 0.01, ***P < 0.001 vs Ctrl (VSMCs without platelets); #P < 0.05, ##P < 0.01, ###P < 0.001 vs HS-APs. (D) The rate of proliferation was determined by EdU incorporation assay in VSMCs transfected with miR-223 mimic. Data are presented as mean ± SD (n = 6). Comparisons between two groups were analyzed using unpaired nonparametric Student’s t-test.*P < 0.05 vs Ctrl (miR-NC).
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