Rabbit Monoclonal N Cadherin antibody. Carrier free. Suitable for IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cadherin antibody [EPR1792Y] - BSA and Azide free (AB239839), expandable thumbnail](https://content.abcam.com/products/images/pan-cadherin-antibody-epr1792y-bsa-and-azide-free-ab239839--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img27981.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cadherin antibody [EPR1792Y] - BSA and Azide free (AB239839)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cadherin antibody [EPR1792Y] - BSA and Azide free (AB239839), expandable thumbnail](https://content.abcam.com/products/images/pan-cadherin-antibody-epr1792y-bsa-and-azide-free-ab239839--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img73258.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cadherin antibody [EPR1792Y] - BSA and Azide free (AB239839)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cadherin antibody [EPR1792Y] - BSA and Azide free (AB239839), expandable thumbnail](https://content.abcam.com/products/images/pan-cadherin-antibody-epr1792y-bsa-and-azide-free-ab239839--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img57016.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cadherin antibody [EPR1792Y] - BSA and Azide free (AB239839)")
![Western blot - Anti-pan Cadherin antibody [EPR1792Y] - BSA and Azide free (AB239839), expandable thumbnail](https://content.abcam.com/products/images/pan-cadherin-antibody-epr1792y-bsa-and-azide-free-ab239839--western-blot-img85276.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-pan Cadherin antibody [EPR1792Y] - BSA and Azide free (AB239839)")
![Immunoprecipitation - Anti-pan Cadherin antibody [EPR1792Y] - BSA and Azide free (AB239839), expandable thumbnail](https://content.abcam.com/products/images/pan-cadherin-antibody-epr1792y-bsa-and-azide-free-ab239839--immunoprecipitation-img690.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-pan Cadherin antibody [EPR1792Y] - BSA and Azide free (AB239839)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | WB | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Expected | Expected | Expected | Expected | Tested |
| Mouse | Tested | Expected | Tested | Expected | Expected |
| Rat | Expected | Tested | Expected | Expected | Expected |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
4 products for Alternative Product
8 products for Alternative Version
Target data
Function
Calcium-dependent cell adhesion protein; preferentially mediates homotypic cell-cell adhesion by dimerization with a CDH2 chain from another cell. Cadherins may thus contribute to the sorting of heterogeneous cell types. Acts as a regulator of neural stem cells quiescence by mediating anchorage of neural stem cells to ependymocytes in the adult subependymal zone: upon cleavage by MMP24, CDH2-mediated anchorage is affected, leading to modulate neural stem cell quiescence. Plays a role in cell-to-cell junction formation between pancreatic beta cells and neural crest stem (NCS) cells, promoting the formation of processes by NCS cells (By similarity). CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density.
Alternative names
Cadherin-2, CDw325, Neural cadherin, N-cadherin, NCAD, CDHN, CDH2
Recommended products
Rabbit Monoclonal N Cadherin antibody. Carrier free. Suitable for IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples.
Alternative names
Cadherin-2, CDw325, Neural cadherin, N-cadherin, NCAD, CDHN, CDH2
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR1792Y
- Purification technique
Affinity purification Protein A
- Specificity
The immunogen used for this product shares 100% homology with N-cadherin, 100% homology with R-cadherin, 93% homology to K-cadherin, 93% homology to P-cadherin, 93% homology to E-cadherin, and 73% homology to VE-cadherin. Cross-reactivities with these proteins have not been confirmed experimentally.
- Dissociation constant
6.1 x 10-10 M
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab239839 is the carrier-free version of Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker ab51034.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Pan Cadherin also known as cadherin pan antibodies or anti pan cadherin is a protein family that mediates cell-cell adhesion in tissues. This protein group includes several members like E-cadherin and N-cadherin which are integral in maintaining the structural integrity of tissues. Pan Cadherin proteins weigh around 120 kDa and are widely expressed in various cell types including epithelial and neural tissues. They possess repetitive amino acid sequences and are calcium-dependent playing an important role in the mechanical linking of cells.
- Biological function summary
Pan Cadherin proteins mediate adhesion through forming homophilic interactions. These proteins are components of adherens junctions which are important structures in cellular adhesion. Within these complexes cadherins interact with catenins to link the cytoskeleton to cell membrane proteins maintaining cell polarity and tissue architecture. The function of cadherins extends to signaling roles that influence cell behavior and differentiation emphasizing their role in cellular communication.
- Pathways
Pan Cadherin proteins participate in significant signaling mechanisms such as the Wnt signaling and epithelial-mesenchymal transition (EMT) pathways. In the Wnt signaling pathway cadherins influence cell fate decisions while in EMT they drive the remodeling of cellular structures important for tissue development. N-Cadherin is closely associated with Pan Cadherin in these pathways collaborating to mediate cellular transformations and migrations essential for embryogenesis and wound healing processes.
- Associated diseases and disorders
Pan Cadherin dysfunction correlates with cancer progression and metastasis. Aberrant cadherin expression contributes to the loss of cell adhesion promoting tumor invasion and metastasis. In particular disruptions in E-Cadherin frequently found in epithelial cancers highlight the critical role of Pan Cadherin in maintaining tissue homeostasis. Additionally N-Cadherin upregulation in various cancers illustrates its involvement in tumor aggressiveness further linking Pan Cadherin to pathogenesis.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
8 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cadherin antibody [EPR1792Y] - BSA and Azide free (ab239839), expandable thumbnail](https://content.abcam.com/products/images/pan-cadherin-antibody-epr1792y-bsa-and-azide-free-ab239839--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img27981.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cadherin antibody [EPR1792Y] - BSA and Azide free (ab239839)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cadherin antibody [EPR1792Y] - BSA and Azide free (ab239839)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat cardiac muscle tissue sections labeling pan Cadherin with purified Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker ab51034 at 1/500 dilution (0.26 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker ab51034).![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cadherin antibody [EPR1792Y] - BSA and Azide free (ab239839), expandable thumbnail](https://content.abcam.com/products/images/pan-cadherin-antibody-epr1792y-bsa-and-azide-free-ab239839--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img73258.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cadherin antibody [EPR1792Y] - BSA and Azide free (ab239839)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cadherin antibody [EPR1792Y] - BSA and Azide free (ab239839)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cardiac muscle tissue sections labeling pan Cadherin with purified Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker ab51034 at 1/500 dilution (0.26 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker ab51034).![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cadherin antibody [EPR1792Y] - BSA and Azide free (ab239839), expandable thumbnail](https://content.abcam.com/products/images/pan-cadherin-antibody-epr1792y-bsa-and-azide-free-ab239839--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img57016.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cadherin antibody [EPR1792Y] - BSA and Azide free (ab239839)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cadherin antibody [EPR1792Y] - BSA and Azide free (ab239839)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cardiac muscle tissue sections labeling pan Cadherin with purified Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker ab51034 at 1/500 dilution (0.26 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker ab51034).![Western blot - Anti-pan Cadherin antibody [EPR1792Y] - BSA and Azide free (ab239839), expandable thumbnail](https://content.abcam.com/products/images/pan-cadherin-antibody-epr1792y-bsa-and-azide-free-ab239839--western-blot-img85276.jpg "Western blot - Anti-pan Cadherin antibody [EPR1792Y] - BSA and Azide free (ab239839)")
Western blot - Anti-pan Cadherin antibody [EPR1792Y] - BSA and Azide free (ab239839)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker ab51034).
All lanes: Western blot - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker ab51034) at 1/50000 dilution
Lane 1: C6 (Rat glial tumor glial cell) whole cell lysate at 20 µg
Lane 2: Human brain lysate at 20 µg
Lane 3: Mouse brain lysate at 20 µg
Lane 4: Rat brain lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 100 kDa
Observed band size: 140 kDa
![Immunoprecipitation - Anti-pan Cadherin antibody [EPR1792Y] - BSA and Azide free (ab239839), expandable thumbnail](https://content.abcam.com/products/images/pan-cadherin-antibody-epr1792y-bsa-and-azide-free-ab239839--immunoprecipitation-img690.jpg "Immunoprecipitation - Anti-pan Cadherin antibody [EPR1792Y] - BSA and Azide free (ab239839)")
Immunoprecipitation - Anti-pan Cadherin antibody [EPR1792Y] - BSA and Azide free (ab239839)
Purified Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker ab51034 at 1/20 dilution (0.6 µg) immunoprecipitating pan Cadherin in Rat heart lysate.
Lane 1 (input): Rat heart lysate 10 µg
Lane 2 (+): Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker ab51034 + Rat heart lysate.
Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker ab51034 in Rat heart lysate.
VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 140 kDa
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker ab51034).All lanes: Immunoprecipitation - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker ab51034)
![Flow Cytometry (Intracellular) - Anti-pan Cadherin antibody [EPR1792Y] - BSA and Azide free (ab239839), expandable thumbnail](https://content.abcam.com/products/images/pan-cadherin-antibody-epr1792y-bsa-and-azide-free-ab239839--flow-cytometry-intracellular-img27363.jpg "Flow Cytometry (Intracellular) - Anti-pan Cadherin antibody [EPR1792Y] - BSA and Azide free (ab239839)")
Flow Cytometry (Intracellular) - Anti-pan Cadherin antibody [EPR1792Y] - BSA and Azide free (ab239839)
Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling pan Cadherin with purified Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker ab51034 at 1/20 dilution (10 μg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker ab51034).
![Western blot - Anti-pan Cadherin antibody [EPR1792Y] - BSA and Azide free (ab239839), expandable thumbnail](https://content.abcam.com/products/images/pan-cadherin-antibody-epr1792y-bsa-and-azide-free-ab239839--western-blot-img97122.jpg "Western blot - Anti-pan Cadherin antibody [EPR1792Y] - BSA and Azide free (ab239839)")
Western blot - Anti-pan Cadherin antibody [EPR1792Y] - BSA and Azide free (ab239839)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker ab51034).
All lanes: Western blot - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker ab51034) at 1/50000 dilution
All lanes: Mouse heart lysate at 15 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 100 kDa
Observed band size: 140 kDa
![OI-RD Scanning - Anti-pan Cadherin antibody [EPR1792Y] - BSA and Azide free (ab239839), expandable thumbnail](https://content.abcam.com/products/images/pan-cadherin-antibody-epr1792y-bsa-and-azide-free-ab239839--oi-rd-scanning-img82383.jpg "OI-RD Scanning - Anti-pan Cadherin antibody [EPR1792Y] - BSA and Azide free (ab239839)")
OI-RD Scanning - Anti-pan Cadherin antibody [EPR1792Y] - BSA and Azide free (ab239839)
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.
![Flow Cytometry (Intracellular) - Anti-pan Cadherin antibody [EPR1792Y] - BSA and Azide free (ab239839), expandable thumbnail](https://content.abcam.com/products/images/pan-cadherin-antibody-epr1792y-bsa-and-azide-free-ab239839--flow-cytometry-intracellular-img27363.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-pan Cadherin antibody [EPR1792Y] - BSA and Azide free (ab239839)")
![Western blot - Anti-pan Cadherin antibody [EPR1792Y] - BSA and Azide free (ab239839), expandable thumbnail](https://content.abcam.com/products/images/pan-cadherin-antibody-epr1792y-bsa-and-azide-free-ab239839--western-blot-img97122.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-pan Cadherin antibody [EPR1792Y] - BSA and Azide free (ab239839)")
![OI-RD Scanning - Anti-pan Cadherin antibody [EPR1792Y] - BSA and Azide free (ab239839), expandable thumbnail](https://content.abcam.com/products/images/pan-cadherin-antibody-epr1792y-bsa-and-azide-free-ab239839--oi-rd-scanning-img82383.jpg/_jcr_content/renditions/webp-475.webp "OI-RD Scanning - Anti-pan Cadherin antibody [EPR1792Y] - BSA and Azide free (ab239839)")
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