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Rabbit Monoclonal N Cadherin antibody. Intercellular Junction marker. Suitable for IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 20 publications.


Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (AB51034), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (AB51034), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (AB51034), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (AB51034), expandable thumbnail
  • Western blot - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (AB51034), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPWBICC/IFFlow Cyt (Intra)
Human
Tested
Expected
Expected
Tested
Tested
Mouse
Tested
Expected
Tested
Expected
Expected
Rat
Tested
Tested
Tested
Expected
Expected

Tested
Tested

Species

Mouse

Dilution info

1/500

Notes

For unpurified use at 1/100.

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Species

Rat

Dilution info

1/500

Notes

For unpurified use at 1/100.

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Species

Human

Dilution info

1/500

Notes

For unpurified use at 1/100.

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Tested
Tested

Species

Rat

Dilution info

1/20

Notes

For unpurified use at 1/40.

Expected
Expected

Species

Mouse, Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/50000

Notes

-

Species

Rat

Dilution info

1/50000

Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100 - 1/250

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

1/20

Notes

For unpurified use at 1/30. Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Associated Products

Select an associated product type

4 products for Alternative Product

Target data

Function

Calcium-dependent cell adhesion protein; preferentially mediates homotypic cell-cell adhesion by dimerization with a CDH2 chain from another cell. Cadherins may thus contribute to the sorting of heterogeneous cell types. Acts as a regulator of neural stem cells quiescence by mediating anchorage of neural stem cells to ependymocytes in the adult subependymal zone: upon cleavage by MMP24, CDH2-mediated anchorage is affected, leading to modulate neural stem cell quiescence. Plays a role in cell-to-cell junction formation between pancreatic beta cells and neural crest stem (NCS) cells, promoting the formation of processes by NCS cells (By similarity). CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density.

Alternative names

Cadherin-2, CDw325, Neural cadherin, N-cadherin, NCAD, CDHN, CDH2

Recommended products

Rabbit Monoclonal N Cadherin antibody. Intercellular Junction marker. Suitable for IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 20 publications.

Alternative names

Cadherin-2, CDw325, Neural cadherin, N-cadherin, NCAD, CDHN, CDH2

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR1792Y

Purification technique

Affinity purification Protein A

Specificity

The immunogen used for this product shares 100% homology with N-cadherin, 100% homology with R-cadherin, 93% homology to K-cadherin, 93% homology to P-cadherin, 93% homology to E-cadherin, and 73% homology to VE-cadherin. Cross-reactivities with these proteins have not been confirmed experimentally.

Dissociation constant

6.1 x 10-10 M

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Pan Cadherin also known as cadherin pan antibodies or anti pan cadherin is a protein family that mediates cell-cell adhesion in tissues. This protein group includes several members like E-cadherin and N-cadherin which are integral in maintaining the structural integrity of tissues. Pan Cadherin proteins weigh around 120 kDa and are widely expressed in various cell types including epithelial and neural tissues. They possess repetitive amino acid sequences and are calcium-dependent playing an important role in the mechanical linking of cells.

Biological function summary

Pan Cadherin proteins mediate adhesion through forming homophilic interactions. These proteins are components of adherens junctions which are important structures in cellular adhesion. Within these complexes cadherins interact with catenins to link the cytoskeleton to cell membrane proteins maintaining cell polarity and tissue architecture. The function of cadherins extends to signaling roles that influence cell behavior and differentiation emphasizing their role in cellular communication.

Pathways

Pan Cadherin proteins participate in significant signaling mechanisms such as the Wnt signaling and epithelial-mesenchymal transition (EMT) pathways. In the Wnt signaling pathway cadherins influence cell fate decisions while in EMT they drive the remodeling of cellular structures important for tissue development. N-Cadherin is closely associated with Pan Cadherin in these pathways collaborating to mediate cellular transformations and migrations essential for embryogenesis and wound healing processes.

Associated diseases and disorders

Pan Cadherin dysfunction correlates with cancer progression and metastasis. Aberrant cadherin expression contributes to the loss of cell adhesion promoting tumor invasion and metastasis. In particular disruptions in E-Cadherin frequently found in epithelial cancers highlight the critical role of Pan Cadherin in maintaining tissue homeostasis. Additionally N-Cadherin upregulation in various cancers illustrates its involvement in tumor aggressiveness further linking Pan Cadherin to pathogenesis.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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12 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat cardiac muscle tissue sections labeling pan Cadherin with purified ab51034 at 1/500 dilution (0.26 μg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cardiac muscle tissue sections labeling pan Cadherin with purified ab51034 at 1/500 dilution (0.26 μg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Immunocytochemistry/ Immunofluorescence - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)

    Immunocytochemistry/Immunofluorescence analysis of MCF7 (human breast adenocarcinoma epithelial cells labeling pan Cadherin with purified ab51034 at 1/250. Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% TritonX-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.

    Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889, Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)1/200 was used as counterstain antibody.

    Confocal image showing membranous staining in MCF7 cells.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cardiac muscle tissue sections labeling pan Cadherin with purified ab51034 at 1/500 dilution (0.26 μg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Western blot - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034), expandable thumbnail

    Western blot - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)

    All lanes: Western blot - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034) at 1/50000 dilution

    Lane 1: C6 (Rat glial tumor glial cell) whole cell lysate at 20 µg

    Lane 2: Human brain lysate at 20 µg

    Lane 3: Mouse brain lysate at 20 µg

    Lane 4: Rat brain lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 100 kDa

    Observed band size: 140 kDa

  • Immunoprecipitation - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034), expandable thumbnail

    Immunoprecipitation - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)

    Purified ab51034 at 1/20 dilution (0.6 μg) immunoprecipitating pan Cadherin in Rat heart lysate.
    Lane 1 (input): Rat heart lysate 10 μg
    Lane 2 (+): ab51034 + Rat heart lysate.
    Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab51034 in Rat heart lysate.
    VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) (1/1000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 140 kDa

    All lanes: Immunoprecipitation - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)

  • Flow Cytometry (Intracellular) - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)

    Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling pan Cadherin with purified ab51034 at 1/20 dilution (10μg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluorr® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)

    Immunohistochemical analysis of Paraffin-embedded human colon tissue sections labeling pan Cadherin with unpurified ab51034 at 1/100. Goat Anti-Rabbit IgG H&L (HRP) was used as the secondary antibody. Sections were counterstained with Hematoxylin. Antigen retrieval was heat mediated using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

    Positive staining on human colon.

  • Western blot - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034), expandable thumbnail

    Western blot - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)

    All lanes: Western blot - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034) at 1/50000 dilution

    All lanes: Mouse heart lysate at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 100 kDa

    Observed band size: 140 kDa

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51034).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)

    Immunohistochemical analysis of Paraffin-embedded rat colon tissue sections labeling pan Cadherin with unpurified ab51034 at 1/100. Goat Anti-Rabbit IgG H&L (HRP) was used as the secondary antibody. Sections were counterstained with Hematoxylin. Antigen retrieval was heat mediated using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

    Positive staining on rat colon.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)

    Immunohistochemical analysis of Paraffin-embedded mouse testis tissue sections labeling pan Cadherin with unpurified ab51034 at 1/100. Goat Anti-Rabbit IgG H&L (HRP) was used as the secondary antibody. Sections were counterstained with Hematoxylin. Antigen retrieval was heat mediated using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

    Positive staining on mouse testis.

  • OI-RD Scanning - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034), expandable thumbnail

    OI-RD Scanning - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

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Product protocols

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