Anti-Pan Cav2 antibody [EPR28073-84] (ab315092)

Rabbit Recombinant Monoclonal CACNA1A antibody. Suitable for WB, IHC-P, IHC-Fr and reacts with Mouse, Rat, Transfected cell lysate - Mouse samples.

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Images

Western blot - Anti-Pan Cav2 antibody [EPR28073-84] (AB315092), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	WB	IHC-P	IHC-Fr	IP	ICC/IF	Flow Cyt
Human	Not recommended	Not recommended	Not recommended	Not recommended	Not recommended	Not recommended
Mouse	Tested	Tested	Tested	Not recommended	Not recommended	Not recommended
Rat	Tested	Tested	Tested	Not recommended	Not recommended	Not recommended
Transfected cell lysate - Mouse	Tested	Not recommended	Not recommended	Not recommended	Not recommended	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/1000	Notes -
Species Rat	Dilution info 1/1000	Notes -
Species Transfected cell lysate - Mouse	Dilution info 1/1000	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/100	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/100	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Transfected cell lysate - Mouse	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/50	Notes -
Species Rat	Dilution info 1/50	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Transfected cell lysate - Mouse	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Rat, Human, Transfected cell lysate - Mouse	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Mouse, Rat, Transfected cell lysate - Mouse	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Mouse, Rat, Transfected cell lysate - Mouse	Dilution info -	Notes -

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

See full target information Cacna1a

Function

Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1A gives rise to P and/or Q-type calcium currents. P/Q-type calcium channels belong to the 'high-voltage activated' (HVA) group and are specifically blocked by the spider omega-agatoxin-IVA (AC P54282) (By similarity). They are however insensitive to dihydropyridines (DHP).

Alternative names

Caca1a, Cach4, Cacn3, Cacnl1a4, Ccha1a, Cacna1a, Voltage-dependent P/Q-type calcium channel subunit alpha-1A, Brain calcium channel I, Voltage-gated calcium channel subunit alpha Cav2.1, BI

Recommended products

Rabbit Recombinant Monoclonal CACNA1A antibody. Suitable for WB, IHC-P, IHC-Fr and reacts with Mouse, Rat, Transfected cell lysate - Mouse samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR28073-84
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Pan Cav2 also known as Cav2 channels or voltage-gated calcium channels of the Cav2 family function mechanically to facilitate the influx of calcium ions through cellular membranes in response to membrane depolarization. These channels are important for converting electrical signals into calcium signals which play a big role in various cellular processes. Cav2 channels typically compose of the α1 subunit and several auxiliary subunits. The α1 subunit has an approximate mass of 190-250 kDa. Cav2 channels express widely throughout the nervous system including in neurons of the brain and spinal cord and also in cardiac and skeletal muscle cells.

Biological function summary

Cav2 channels play key roles in neurotransmitter release muscle contraction and hormone secretion. These channels form part of multiprotein complexes that involve various auxiliary subunits which modulate their function and localization. Through their regulation of calcium influx Cav2 channels critically impact synaptic transmission and plasticity in neuronal communication. They also contribute to excitation-contraction coupling in muscle cells enabling the precise control of muscle actions.

Pathways

Cav2 channels heavily influence calcium signaling pathways and synaptic plasticity pathways. Within the calcium signaling pathway their activity directly alters intracellular calcium concentrations which modulate downstream signaling cascades. These channels closely interact with proteins such as SNARE proteins and synaptotagmin in neurotransmitter release. In pathways related to synaptic plasticity Cav2 channels influence long-term potentiation impacting learning and memory processes.

Associated diseases and disorders

Cav2 channel dysfunction associates with neurological disorders such as migraine and epilepsy. Aberrant expression or mutation of Cav2 channel components disrupts normal neural excitation leading to these disorders. The channels also connect with auxiliary proteins such as α2δ subunits that can alter pain pathways implicating them in certain pain disorders. Moreover irregularities in Cav2 channel function may link to cardiac arrhythmias through interactions with cardiac proteins responsible for proper heartbeat rhythm.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

12 product images

Western blot - Anti-Pan Cav2 antibody [EPR28073-84] (ab315092)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
This antibody reacts with CAV2.2 and CAV2.3.
Samples are non-boiled as boiling may cause protein aggregation.
In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.
All lanes: Western blot - Anti-Pan Cav2 antibody [EPR28073-84] (ab315092) at 1/1000 dilution
Lane 1: 293T cells transfected with an empty vector containing a His-tag, whole cell lysate at 10 µg
Lane 2: 293T cells transfected with a mouse CAV2.1 expression vector containing a His-tag, whole cell lysate at 10 µg
Lane 3: 293T cells transfected with a mouse CAV2.2 expression vector containing a His-tag, whole cell lysate at 10 µg
Lane 4: 293T cells transfected with a mouse CAV2.3 expression vector containing a His-tag, whole cell lysate at 10 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 100,110,120 kDa
Exposure time: 10s
Western blot - Anti-Pan Cav2 antibody [EPR28073-84] (ab315092)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: spleen, liver(PMID: 8929530)
Samples are non-boiled as boiling may cause protein aggregation.
The identity of the lower MW band at approximately 100 kDa may represent an N-terminal truncated isoform of CACNA1A (PMID: 7673157).
In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] ab129002) staining at 1/10000 dilution.
All lanes: Western blot - Anti-Pan Cav2 antibody [EPR28073-84] (ab315092) at 1/1000 dilution
Lane 1: Rat brain tissue lysate at 20 µg
Lane 2: Rat spleen tissue lysate at 20 µg
Lane 3: Rat liver tissue lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 100, 267 kDa, 124 kDa
Exposure time: 103s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pan Cav2 antibody [EPR28073-84] (ab315092)
Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling Pan Cav2 with ab315092 at 1/100 (5.16 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: no staining on rat liver. The section was incubated with ab315092 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pan Cav2 antibody [EPR28073-84] (ab315092)
Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Pan Cav2 with ab315092 at 1/100 (5.16 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: no staining on mouse liver. The section was incubated with ab315092 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pan Cav2 antibody [EPR28073-84] (ab315092)
Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling Pan Cav2 with ab315092 at 1/100 (5.16 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on rat cerebrum. The section was incubated with ab315092 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pan Cav2 antibody [EPR28073-84] (ab315092)
Immunohistochemical analysis of paraffin-embedded Mouse cerebellum tissue labeling Pan Cav2 with ab315092 at 1/100 (5.16 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on mouse cerebellum. The section was incubated with ab315092 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pan Cav2 antibody [EPR28073-84] (ab315092)
Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Pan Cav2 with ab315092 at 1/100 (5.16 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on mouse cerebrum. The section was incubated with ab315092 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Frozen sections) - Anti-Pan Cav2 antibody [EPR28073-84] (ab315092)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat liver (fresh) tissue labeling Pan Cav2 with ab315092 at 1/50 (10.32 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green).
Negative control: confocal image showing no staining on rat liver. The section was incubated with ab315092 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 (2 ug/mL) dilution.
Immunohistochemistry (Frozen sections) - Anti-Pan Cav2 antibody [EPR28073-84] (ab315092)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat cerebellum (fresh) tissue labeling Pan Cav2 with ab315092 at 1/50 (10.32 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green).
Confocal image showing positive staining on rat cerebellum. The section was incubated with ab315092 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 (2 ug/mL) dilution.
Immunohistochemistry (Frozen sections) - Anti-Pan Cav2 antibody [EPR28073-84] (ab315092)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse liver (fresh) tissue labeling Pan Cav2 with ab315092 at 1/50 (10.32 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green).
Negative control: confocal image showing no staining on mouse liver. The section was incubated with ab315092 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 (2 ug/mL) dilution.
Western blot - Anti-Pan Cav2 antibody [EPR28073-84] (ab315092)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: liver, testis(PMID: 8929530)
Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.
Samples are non-boiled as boiling may cause protein aggregation.
The identity of the lower MW band at approximately 100 kDa may represent an N-terminal truncated isoform of CACNA1A (PMID: 7673157).
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-Pan Cav2 antibody [EPR28073-84] (ab315092) at 1/1000 dilution
Lane 1: Mouse brain tissue lysate at 20 µg
Lane 2: Mouse liver tissue lysate at 20 µg
Lane 3: Mouse testis tissue lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 100, 267 kDa, 36 kDa
Exposure time: 180s
Immunohistochemistry (Frozen sections) - Anti-Pan Cav2 antibody [EPR28073-84] (ab315092)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebellum (fresh) tissue labeling Pan Cav2 with ab315092 at 1/50 (10.32 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green).
Confocal image showing positive staining on mouse cerebellum. The section was incubated with ab315092 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 (2 ug/mL) dilution.