JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB26283

Anti-pan CEACAM antibody [TET2]

Be the first to review this product! Submit a review

|

(4 Publications)

Mouse Monoclonal CEACAM5 antibody. Suitable for Flow Cyt and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human CEACAM5.

View Alternative Names

CD66e, CEA, CEACAM5, Cell adhesion molecule CEACAM5, Carcinoembryonic antigen, Carcinoembryonic antigen-related cell adhesion molecule 5, Meconium antigen 100, CEA cell adhesion molecule 5

1 Images
Flow Cytometry - Anti-pan CEACAM antibody [TET2] (AB26283)
  • Flow Cyt

Unknown

Flow Cytometry - Anti-pan CEACAM antibody [TET2] (AB26283)

Overlay histogram showing Caco 2 cells stained with ab26283 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab26283, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

TET2

Isotype

IgG2b

Carrier free

No

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Native Full Length Protein corresponding to Human CEACAM5.

P06731

Specificity

ab26283 is a broadly reactive antibody which reacts with most of the CEACAM molecules characterized to date (except CEACAM3, CEACAM4 and CEACAM7)4. It can be used to detect CEACAM1 (BGP/CD66a), CEACAM5 (CEA/CD66e), CEACAM6 (NCA/CD66c) and CEACAM8 (CGM6/CD66b) on transiently transfected BOSC23 cells by flow cytometry.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg2b-kappa-monoclonal-7e10g10-isotype-control-ab170192'>ab170192</a> - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.</p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
pH: 7.2 Preservative: 0.01% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Pan CEACAM also known as Carcinoembryonic Antigen-related Cell Adhesion Molecule represents a group of glycoproteins. It carries a molecular weight varying between 60 to 90 kDa depending on the glycosylation extent. These molecules express mainly on epithelial cells especially those in the gastrointestinal tract and respiratory system. CEACAMs serve in cell-cell adhesion playing significant roles in maintaining tissue architecture and intercellular communication.
Biological function summary

These proteins participate in immunoregulation and cellular adhesion. CEACAMs function as part of larger multi-protein complexes at the cell surface interacting with numerous ligands and receptors to modulate immune responses. These proteins can inhibit or promote cellular processes such as proliferation differentiation and apoptosis. This way they help in maintaining normal cellular function and adaptation to environmental changes.

Pathways

Pan CEACAM proteins influence pathways related to cellular communication and immune response. They involve particularly in the JAK/STAT and MAPK pathways. CEACAMs participate in initiating or modulating cascade signaling which involves proteins like STAT3 and ERK contributing to versatile cellular responses. These pathways help fine-tune cellular proliferation and survival particularly in response to external stimuli or stress.

Pan CEACAMs hold significant implications in cancer and inflammatory diseases. Elevated CEACAM expression associates with several types of cancer including colorectal and lung cancer where it affects tumor progression and metastasis. It also connects with inflammatory bowel disease contributing to an altered immune response and epithelial damage. In these conditions CEACAMs interact with other proteins such as CEACAM1 and CEACAM5 influencing disease development and progression.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Cell surface glycoprotein that plays a role in cell adhesion, intracellular signaling and tumor progression (PubMed : 10864933, PubMed : 10910050, PubMed : 2803308). Mediates homophilic and heterophilic cell adhesion with other carcinoembryonic antigen-related cell adhesion molecules, such as CEACAM6 (PubMed : 2803308). Plays a role as an oncogene by promoting tumor progression; induces resistance to anoikis of colorectal carcinoma cells (PubMed : 10910050).. (Microbial infection) Receptor for E.coli Dr adhesins. Binding of E.coli Dr adhesins leads to dissociation of the homodimer.
See full target information CEACAM5
style
left-column

Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Cancer research 77:6069-6082 PubMed28923852

2017

TET-Mediated Sequestration of miR-26 Drives EZH2 Expression and Gastric Carcinogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Min Deng,Ruixin Zhang,Zhengxi He,Qinwei Qiu,Xihong Lu,Jiang Yin,Hao Liu,Xiaoting Jia,Zhimin He

International journal of oncology 42:1822-32 PubMed23483119

2013

Modulation of intracellular iron metabolism by iron chelation affects chromatin remodeling proteins and corresponding epigenetic modifications in breast cancer cells and increases their sensitivity to chemotherapeutic agents.

Applications

Unspecified application

Species

Unspecified reactive species

Igor P Pogribny,Volodymyr P Tryndyak,Marta Pogribna,Svitlana Shpyleva,Gordon Surratt,Gonçalo Gamboa da Costa,Frederick A Beland

BMC genomics 12:295 PubMed21645414

2011

Characterization of killer immunoglobulin-like receptor genetics and comprehensive genotyping by pyrosequencing in rhesus macaques.

Applications

IP

Species

Rhesus monkey

Anna J Moreland,Lisbeth A Guethlein,R Keith Reeves,Karl W Broman,R Paul Johnson,Peter Parham,David H O'Connor,Benjamin N Bimber

The Journal of biological chemistry 286:24336-49 PubMed21561871

2011

Identification of neutrophil granule glycoproteins as Lewis(x)-containing ligands cleared by the scavenger receptor C-type lectin.

Applications

WB

Species

Unspecified reactive species

Sarah A Graham,Aristotelis Antonopoulos,Paul G Hitchen,Stuart M Haslam,Anne Dell,Kurt Drickamer,Maureen E Taylor
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB4567

Anti-pan CEACAM antibody [D14HD11]

  • 0
  • 0

Complementary Products

Assay Kits

AB264604

Human CEA ELISA Kit

Recombinant|SimpleStep

ELISA - Human CEA ELISA Kit (AB264604)

  • 0
  • 0
Primary Antibodies

AB133633

Anti-Carcino Embryonic Antigen CEA antibody [EPCEAR7]

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carcino Embryonic Antigen CEA antibody [EPCEAR7] (AB133633)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB205270

Anti-active YAP1 antibody [EPR19812]

KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Western blot - Anti-active YAP1 antibody [EPR19812] (AB205270)

  • 5
  • 25
Primary Antibodies

AB32072

Anti-c-Myc antibody [Y69] - ChIP Grade

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Myc antibody [Y69] - ChIP Grade (AB32072)

  • 4
  • 56

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com