Name: Anti-pan Cytokeratin antibody [AE1/AE3 + 5D3]
SKU: ab86734
Rating: 5 (11 reviews)

Anti-pan Cytokeratin antibody [AE1/AE3 + 5D3] (ab86734) is a mouse recombinant multiclonal antibody detecting pan Cytokeratin in Flow Cytometry, IHC-P, ICC/IF. Suitable for Chicken, Dog, Human, Pig.

- Recombinant format for unrivalled batch-batch consistency

- Over 60 publications

- Trusted since 2010

Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cytokeratin antibody [AE1/AE3 + 5D3] (AB86734), expandable thumbnail

Publications

Key facts

Isotype: IgG1
Host species: Mouse
Storage buffer: Preservative: 0.09% Sodium azide
Form: Liquid
Clonality: Multiclonal

Immunogen

Full Length Protein corresponding to Human KRT8. The exact immunogen used to generate this antibody is proprietary information. Database link P05787

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IHC-P	Flow Cyt	ICC/IF
Human	Tested	Tested	Tested
Chicken	Tested	Expected	Expected
Dog	Tested	Expected	Expected
Goat	Tested	Expected	Expected
Pig	Tested	Expected	Expected

Tested
Tested

Species	Dilution info	Notes
Species Chicken	Dilution info 1/250	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Species Dog	Dilution info 1/250	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Species Goat	Dilution info 1/250	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Species Pig	Dilution info -	Notes -
Species Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/100	Notes ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species	Dilution info	Notes
Species Chicken, Dog, Goat, Pig	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/100	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Chicken, Dog, Goat, Pig	Dilution info Use at an assay dependent concentration.	Notes -

Associated Products

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14 products for Alternative Product

Target data

See full target information KRT8

Function

Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.

Additional Targets

KRT18

Alternative names

CYK8, KRT8, Cytokeratin-8, Keratin-8, Type-II keratin Kb8, CK-8, K8

Key facts

Isotype: IgG1
Host species: Mouse
Storage buffer: Preservative: 0.09% Sodium azide
Form: Liquid
Clonality: Multiclonal
Immunogen: Full Length Protein corresponding to Human KRT8. The exact immunogen used to generate this antibody is proprietary information. Database link P05787
Clone number: AE1/AE3 + 5D3
Purification technique: Affinity purification Protein A/G
Specificity: The ab86734 is a combination of [AE1/AE3] and [5D3] clones and can be used to detect most human epithelia. [AE1/AE3] recognizes acidic and basic subfamilies of cytokeratins, with molecular weights ranging from 40 to 67 kDa. [5D3] recognizes Cytokeratin 8 and 18 intermediate filament proteins. In normal tissues, [5D3] recognizes all simple and glandular epithelium. It has been observed that [AE1/AE3] has had problems marking certain tissues types and adenocarcinomas. The addition of [5D3] may remedy some of the limitations observed when staining with [AE1/AE3] alone.
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Storage information: Avoid freeze / thaw cycle

Notes

Product Specifications
Anti-pan Cytokeratin antibody [AE1/AE3 + 5D3] (ab86734) is a recombinant mouse multiclonal antibody which is comprised of multiple monoclonal antibodies targeting different epitopes and recapitulating the performance of a polyclonal.
Anti-pan Cytokeratin antibody [AE1/AE3 + 5D3] (ab86734) is validated for use in Flow Cyt, ICC/IF and IHC-P in chicken, dog, human and pig samples.
Anti-pan Cytokeratin antibody [AE1/AE3 + 5D3] (ab86734) specifically detects pan Cytokeratin/ Keratin, type II cytoskeletal 1b (UniProt ID: Q7Z794; Molecular weight: 62kDa) and is sold in a 100 µL selling size.

Quality and Validation
Abcams high quality manufacturing and validation processes ensure anti-pan Cytokeratin antibody [AE1/AE3 + 5D3] (ab86734) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.

This product was changed from ascites to tissue culture supernatant on 8th March 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

What is an oligoclonal antibody?
This oligoclonal antibody is a cocktail of monoclonal antibodies that have been carefully selected. Oligoclonal antibodies have not only the specificity and batch-to-batch consistency of a monoclonal antibody, but also have the advantage of the sensitivity of a polyclonal antibody due to their ability to recognize multiple epitopes on an antigen.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Pan cytokeratin also known as cytokeratin AE1/AE3 is a cytoskeletal protein complex that consists of different keratin polypeptides. It mechanically supports epithelial cells contributing to their structural integrity. The protein mass is variable due to its composite nature ranging between 40 to 67 kDa. Pan cytokeratin is expressed in epithelial tissues throughout the body serving as a reliable marker for identifying epithelial origin cells in histological studies.

Biological function summary

Keratins in pan cytokeratin contribute to maintaining structural stability within epithelial cells. They act as components of the cytoskeletal network interacting with other cellular structures to provide protection from stress and mechanical damage. Pan cytokeratin exists as part of the intermediate filament protein complex which plays a significant role in cellular mechanics and cohesion essential for tissue integrity.

Pathways

Intermediate filaments containing pan cytokeratin participate in pathways related to cell differentiation and stress response. Its interactions with other proteins like desmoplakin and plectin link the cytoskeletal filaments to cell membranes and the extracellular matrix. This involvement is important in pathways related to cellular structural integrity and epithelial-mesenchymal transition (EMT).

Associated diseases and disorders

Pan cytokeratin serves as a critical marker in the diagnosis of epithelial-derived cancers such as carcinoma and conditions like psoriasis. Abnormal expression patterns of pan cytokeratin indicate tumor presence and origin. Its connection to proteins such as E-cadherin and vimentin assists in understanding the progression and metastasis of cancer by facilitating EMT and tissue invasion diagnostics.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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8 product images

This image is courtesy of a customer review submitted by Carl Hobbs
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cytokeratin antibody [AE1/AE3 + 5D3] (ab86734)
ab86734 staining pan Cytokeratin in pig small intestine tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in citric acid. Samples were incubated with primary antibody (1/250 in TBS/BSA/azide) for 2 hours at 21°C. A Biotin-conjugated goat anti-mouse IgG polyclonal (1/200) was used as the secondary antibody.
Mr Carl Hobbs
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cytokeratin antibody [AE1/AE3 + 5D3] (ab86734)
Formaldehyde fixed dog lung tissue stained for pan Cytokeratin with ab86734 at a 1/250 dilution. Heat mediated - Buffer/Enzyme Used: Citric acid. 1% BSA used for blocking for 10 minutes at RT. Primary incubation for 2 hours at RT in TBS/BSA/Azide. Secondary: Goat polyclonal conjugated to biotin used at a 1/200 dilution.
Mr Carl Hobbs
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cytokeratin antibody [AE1/AE3 + 5D3] (ab86734)
Formaldehyde fixed chicken lung tissue stained for pan Cytokeratin with ab86734 at a 1/200 dilution. Heat mediated - Buffer/Enzyme Used: Citric acid. 1% BSA used for blocking for 10 minutes at RT. Primary incubation for 16 hours at RT in TBS/BSA/Azide. Secondary: Goat polyclonal conjugated to biotin used at a 1/200 dilution.
Flow Cytometry - Anti-pan Cytokeratin antibody [AE1/AE3 + 5D3] (ab86734)
Overlay histogram showing A431 cells stained with ab86734 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab86734, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control ab91353, 2µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.
Mr Carl Hobbs
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cytokeratin antibody [AE1/AE3 + 5D3] (ab86734)
Formaldehyde fixed goat lung tissue stained for pan Cytokeratin with ab86734 at a 1/250 dilution. Heat mediated - Buffer/Enzyme Used: Citric acid. 1% BSA used for blocking for 10 minutes at RT. Primary incubation for 2 hours at RT in TBS/BSA/Azide. Secondary: Goat polyclonal conjugated to biotin used at a 1/200 dilution.
This image is courtesy of a customer review submitted by Carl Hobbs
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cytokeratin antibody [AE1/AE3 + 5D3] (ab86734)
ab8673 staining human skin sections by IHC-P. The tissue was fixed with formaldehyde and a heat mediated antigen retrival step was performed with citric acid pH 6. Blocking of the sample was done with 1% BSA for 10 minutes at 21°C, followed by staining with ab8673 at 1/250 in TBS/BSA/azide for 2h at 21°C. A biotinylated goat anti-mouse polyclonal antibody at 1/200 was used as the secondary antibody.
Immunocytochemistry/ Immunofluorescence - Anti-pan Cytokeratin antibody [AE1/AE3 + 5D3] (ab86734)
ab86734 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab86734 at 1/100 dilution overnight at +4°C. The secondary antibody (green) was DyLight^® 488 goat anti- mouse (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor^® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM. This antibody also gave a positive result in methanol fixed (100%, 5min) HeLa, Hek293. HepG2, and MCF-7 cells, also in formaldehyde fixed (4%, 10min) HeLa, Hek293, and MCF-7 cells at 1/100 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cytokeratin antibody [AE1/AE3 + 5D3] (ab86734)
Skin stained for pan Cytokeratin with ab86734 at a 1/100 dilution.