Anti-pan Cytokeratin antibody [AE1/AE3] - BSA and Azide free

7 product images

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  This image is courtesy of the Single Cell & Imaging Mass Cytometry Analysis Platform, Goodman Cancer Research Centre, McGill University

  Mass Cytometry - Anti-pan Cytokeratin antibody [AE1/AE3] - BSA and Azide free (ab80826)

  Imaging Mass Cytometry™ (IMC™) image of human lung cancer tissue stained with Anti-pan Cytokeratin antibody [AE1/AE3]. ab80826 (carrier-free antibody, purified) was metal-conjugated using a Maxpar® Antibody Labeling Kit from Fluidigm. Immunostaining was performed according to Fluidigm's protocols. Briefly, slides were subject to deparaffinization and heat-induced epitope retrieval, followed by overnight incubation at 4°C with an antibody cocktail containing metal-tagged antibodies in blocking buffer. Slides were subsequently washed with 0.2% Triton-X and 1x PBS, counterstained with Cell-ID™ Intercalator-Ir diluted at 1/400 in 1x PBS for 30 min at room temperature, rinsed for 5 min with distilled H2O, and air-dried prior to IMC™ acquisition. IMC™ acquisition was performed using the Fluidigm Hyperion™ Imaging System.
  

  Imaging Mass Cytometry™, IMC™, Cell-ID™, Hyperion™ and Maxpar® are trademarks of Fluidigm Canada

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  Flow Cytometry - Anti-pan Cytokeratin antibody [AE1/AE3] - BSA and Azide free (ab80826)

  Overlay histogram showing A431 cells stained with ab80826 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab80826, 1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control ab91353, 2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.
  
  

  This image was generated using the ascites version of the product.

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  Flow Cytometry - Anti-pan Cytokeratin antibody [AE1/AE3] - BSA and Azide free (ab80826)

  Flow Cytometry analysis of HeLa cells labeling pan Cytokeratin with ab80826 (red). Cells without primary incubation (black) and Isotype Control (green).

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cytokeratin antibody [AE1/AE3] - BSA and Azide free (ab80826)

  Immunohistochemical analysis of formalin-fixed, paraffin-embedded human colon carcinoma tissue with ab80826.

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  Immunocytochemistry/ Immunofluorescence - Anti-pan Cytokeratin antibody [AE1/AE3] - BSA and Azide free (ab80826)

  ICC/IF image of ab80826 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab80826, 5μg/ml) overnight at +4°C. The secondary antibody (green) was Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.
  

  This image was generated using the ascites version of the product.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cytokeratin antibody [AE1/AE3] - BSA and Azide free (ab80826)

  Immunohistochemical analysis of formalin-fixed, paraffin-embedded human tonsil tissue with ab80826 at 1/400 dilution. Anti-Mouse HRP polymer was used as the secondary detection system. Heat-mediated antigen retrieval was performed using citrate based pH 6.0 buffer.
  

  This image was generated using the ascites version of the product.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cytokeratin antibody [AE1/AE3] - BSA and Azide free (ab80826)

  Immunohistochemistry analysis of formalin-fixed, paraffin-embedded Human skin tissue using 1/50 ab80826, a peroxidase-conjugated secondary antibody and an AEC chromogen. Note cytoplasmic staining of epithelial cells.
  

  This image was generated using the ascites version of the product.