AB961

Anti-pan Cytokeratin antibody [AE1+AE3], prediluted

Recombinant
What is this?

(3 Reviews)

(42 Publications)

Mouse Recombinant Multiclonal Cytokeratin 1 antibody AE1+AE3. Suitable for IHC-P, ICC/IF and reacts with Mouse, Rat, Fish, Zebrafish, Chicken, Human, Dog, Cat, Cow, Rabbit samples. Cited in 42 publications.

View Alternative Names

KRTA, KRT1, 67 kDa cytokeratin, Cytokeratin-1, Hair alpha protein, Keratin-1, Type-II keratin Kb1, CK-1, K1

Key facts

Host species

Mouse

Clonality

Multiclonal

Clone number

AE1+AE3

Isotype

IgG1

Carrier free

Reacts with

Mouse, Rat, Rabbit, Chicken, Cow, Cat, Dog, Human, Fish, Zebrafish

Applications

IHC-P, ICC/IF

applications

Specificity

This antibody mixture recognizes acidic and basic subfamilies of cytokeratins. The cocktail of these two antibodies can be used to detect most human epithelia. The acidic cytokeratins have molecular weights of 56.5, 55, 51, 50, 50, 48 46, 45, and 40 kDa. The basic cytokeratins have molecular weights of 65-67, 64, 59, 58, 56 and 52 kDa.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chicken": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Fish": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rabbit": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Zebrafish": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

Have you thought about this alternative?

AB86734

Anti-pan Cytokeratin antibody [AE1/AE3 + 5D3]

11 Reviews

View product

Why is this recommended?

We’re constantly upgrading our range to offer the highest quality products. We have selected this alternative product for your review because it gives improved performance, is easier and more convenient to use, or is available faster.

style

left-column

Product details

This product was changed from ascites to tissue culture supernatant on 8th March 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

What is an oligoclonal antibody?
This oligoclonal antibody is a cocktail of monoclonal antibodies that have been carefully selected. Oligoclonal antibodies have not only the specificity and batch-to-batch consistency of a monoclonal antibody, but also have the advantage of the sensitivity of a polyclonal antibody due to their ability to recognize multiple epitopes on an antigen.

style

left-column

Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Pan cytokeratin also known as cytokeratin AE1/AE3 is a cytoskeletal protein complex that consists of different keratin polypeptides. It mechanically supports epithelial cells contributing to their structural integrity. The protein mass is variable due to its composite nature ranging between 40 to 67 kDa. Pan cytokeratin is expressed in epithelial tissues throughout the body serving as a reliable marker for identifying epithelial origin cells in histological studies.

Biological function summary

Keratins in pan cytokeratin contribute to maintaining structural stability within epithelial cells. They act as components of the cytoskeletal network interacting with other cellular structures to provide protection from stress and mechanical damage. Pan cytokeratin exists as part of the intermediate filament protein complex which plays a significant role in cellular mechanics and cohesion essential for tissue integrity.

Pathways

Intermediate filaments containing pan cytokeratin participate in pathways related to cell differentiation and stress response. Its interactions with other proteins like desmoplakin and plectin link the cytoskeletal filaments to cell membranes and the extracellular matrix. This involvement is important in pathways related to cellular structural integrity and epithelial-mesenchymal transition (EMT).

Pan cytokeratin serves as a critical marker in the diagnosis of epithelial-derived cancers such as carcinoma and conditions like psoriasis. Abnormal expression patterns of pan cytokeratin indicate tumor presence and origin. Its connection to proteins such as E-cadherin and vimentin assists in understanding the progression and metastasis of cancer by facilitating EMT and tissue invasion diagnostics.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

May regulate the activity of kinases such as PKC and SRC via binding to integrin beta-1 (ITB1) and the receptor of activated protein C kinase 1 (RACK1). In complex with C1QBP is a high affinity receptor for kininogen-1/HMWK.

See full target information KRT1

Additional targets

KRT16,Keratin, type I cytoskeletal 14,Keratin, type I cytoskeletal 9,KRT17,KRT4,KRT2,Keratin, type II cytoskeletal 5,Keratin, type I cytoskeletal 13,KRT6A,KRT12,KRT3,KRT10,KRT15

style

left-column

Publications (42)

Recent publications for all applications. Explore the full list and refine your search

BMC veterinary research 19:223 PubMed37880653

2023

Anti-cancer effect of palmatine through inhibition of the PI3K/AKT pathway in canine mammary gland tumor CMT-U27 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Min-Jae Yoo,Jawun Choi,Ye-Ji Jang,Sang-Youel Park,Jae-Won Seol

International journal of molecular sciences 24: PubMed36613940

2022

A New Indicator to Differentiate Thyroid Follicular Inclusions in Cervical Lymph Nodes from Patients with Thyroid Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Chieko Otsubo,Zhanna Mussazhanova,Hirokazu Kurohama,Gulzira Shalgimbayeva,Nozomi Ueki,Yuki Matsuoka,Madina Madiyeva,Shinya Sato,Hiroyuki Yamashita,Masahiro Nakashima

Evidence-based complementary and alternative medicine : eCAM 2022:6295910 PubMed36110191

2022

Methyl Gallate Suppresses Tumor Development by Increasing Activation of Caspase3 and Disrupting Tumor Angiogenesis in Melanoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jeong-Ki Park,Min-Jae Yoo,Hyuk Jang,Sang-Youel Park,Jawun Choi,Jae-Won Seol

Translational andrology and urology 11:761-772 PubMed35812200

2022

Three new experimental models of anterior urethral stricture in rabbits.

Applications

Unspecified application

Species

Unspecified reactive species

Hai-Jun Yao,Zi-Wei Wei,Xiang Wan,Yu-Cheng Tao,Da-Chao Zheng,Zhong Wang,Min-Kai Xie

Methods in molecular biology (Clifton, N.J.) 2528:271-276 PubMed35704197

2022

Immunostaining and Protein Pull-Down of Methyl-5-Cytosine-Marked RNA:DNA Hybrids.

Applications

Unspecified application

Species

Unspecified reactive species

Haibo Yang,Li Lan

ImmunoHorizons 6:253-272 PubMed35440514

2022

a New IFN-Inducible Human Member of the Ly6a Subfamily Expressed by Spleen Cells and Associated with Inflammation and Viral Resistance.

Applications

Unspecified application

Species

Unspecified reactive species

Moriya Shmerling,Michael Chalik,Nechama I Smorodinsky,Alan Meeker,Sujayita Roy,Orit Sagi-Assif,Tsipi Meshel,Artem Danilevsky,Noam Shomron,Shmuel Levinger,Bar Nishry,David Baruchi,Avital Shargorodsky,Ravit Ziv,Avital Sarusi-Portuguez,Maoz Lahav,Marcelo Ehrlich,Bryony Braschi,Elspeth Bruford,Isaac P Witz,Daniel H Wreschner

Stem cell research & therapy 11:535 PubMed33308306

2020

Hypoxia-preconditioned adipose-derived stem cells combined with scaffold promote urethral reconstruction by upregulation of angiogenesis and glycolysis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiang Wan,Min-Kai Xie,Huan Xu,Zi-Wei Wei,Hai-Jun Yao,Zhong Wang,Da-Chao Zheng

Developmental dynamics : an official publication of the American Association of Anatomists 250:175-190 PubMed32877571

2020

The dynamics of epidermal stratification during post-larval development in zebrafish.

Applications

Unspecified application

Species

Unspecified reactive species

Emma Rangel-Huerta,Aida Guzman,Ernesto Maldonado

Cell death & disease 11:267 PubMed32327627

2020

Reprogramming of lipid metabolism in cancer-associated fibroblasts potentiates migration of colorectal cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jin Gong,Yiyun Lin,Huaqin Zhang,Chunqi Liu,Zhong Cheng,Xiaowei Yang,Jiamei Zhang,Yuanyuan Xiao,Na Sang,Xinying Qian,Liang Wang,Xiaobo Cen,Xiao Du,Yinglan Zhao

Chinese medical journal 133:424-434 PubMed31977553

2020

Encapsulated three-dimensional bioprinted structure seeded with urothelial cells: a new construction technique for tissue-engineered urinary tract patch.

Applications

Unspecified application

Species

Unspecified reactive species

Yi-Peng Jin,Chong Shi,Yuan-Yi Wu,Ji-Lei Sun,Jiang-Ping Gao,Yong Yang

View all publications

style

left-column

style

left-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

style

left-column

style

primary-antibodies

pan-cytokeratin-antibody-krt-1877r-ab234297

(6 reviews)

style

bg-slate-100

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com