Anti-pan cytokeratin antibody [EPR28285-45]

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan cytokeratin antibody [EPR28285-45] (AB308262)

Immunohistochemical analysis of paraffin-embedded human liver tissue labeling pan cytokeratin with ab308262 at 1/5000 dilution (0.108 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on human liver. The section was incubated with ab308262 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan cytokeratin antibody [EPR28285-45] (AB308262)

Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma tissue labeling pan cytokeratin with ab308262 at 1/5000 dilution (0.108 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on human adenocarcinoma of colon. The section was incubated with ab308262 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan cytokeratin antibody [EPR28285-45] (AB308262)

Immunohistochemical analysis of paraffin-embedded cell pellets labeling pan cytokeratin with ab308262 at 1/5000 dilution (0.108 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Cytoplasmic staining on (A) HeLa cell pellet. No staining on (B) Raji cell pellet. The section was incubated with ab308262 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan cytokeratin antibody [EPR28285-45] (AB308262)

Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue labeling pan cytokeratin with ab308262 at 1/5000 dilution (0.108 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Negative control : No staining on human skeletal muscle. The section was incubated with ab308262 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan cytokeratin antibody [EPR28285-45] (AB308262)

Immunohistochemical analysis of paraffin-embedded human skin tissue labeling pan cytokeratin with ab308262 at 1/5000 dilution (0.108 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on human skin (PMID : 25411189). The section was incubated with ab308262 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan cytokeratin antibody [EPR28285-45] (AB308262)

Immunohistochemical analysis of paraffin-embedded human colon tissue labeling pan cytokeratin with ab308262 at 1/5000 dilution (0.108 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on human colon. The section was incubated with ab308262 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan cytokeratin antibody [EPR28285-45] (AB308262)

Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue labeling pan cytokeratin with ab308262 at 1/5000 dilution (0.108 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Negative control : No staining on rat skeletal muscle. The section was incubated with ab308262 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan cytokeratin antibody [EPR28285-45] (AB308262)

Immunohistochemical analysis of paraffin-embedded rat skin tissue labeling pan cytokeratin with ab308262 at 1/5000 dilution (0.108 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on rat skin. The section was incubated with ab308262 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan cytokeratin antibody [EPR28285-45] (AB308262)

Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue labeling pan cytokeratin with ab308262 at 1/5000 dilution (0.108 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Negative control : No staining on mouse skeletal muscle. The section was incubated with ab308262 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan cytokeratin antibody [EPR28285-45] (AB308262)

Immunohistochemical analysis of paraffin-embedded mouse skin tissue labeling pan cytokeratin with ab308262 at 1/5000 dilution (0.108 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on mouse skin. The section was incubated with ab308262 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• WB

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Western blot - Anti-pan cytokeratin antibody [EPR28285-45] (AB308262)

Blocking and diluting buffer and concentration : 5% NFDM/TBST. Low expression : skeletal muscle, testis. In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : 10 seconds.

All lanes:

Western blot - Anti-pan cytokeratin antibody [EPR28285-45] (ab308262) at 1/1000 dilution

Lane 1:

Human liver tissue lysate at 20 µg

Lane 2:

Human colon tissue lysate at 20 µg

Lane 3:

Human skeletal muscle tissue lysate at 20 µg

Lane 4:

Human testis tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 37-65 kDa

false

Exposure time: 10s

• WB

Supplier Data

Western blot - Anti-pan cytokeratin antibody [EPR28285-45] (AB308262)

Blocking and diluting buffer and concentration : 5% NFDM/TBST. Low expression : MOLT-4, Ramos, Raji. In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : 10 seconds.

All lanes:

Western blot - Anti-pan cytokeratin antibody [EPR28285-45] (ab308262) at 1/1000 dilution

Lane 1:

A431 (human epidermoid carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

MOLT-4 (human lymphoblastic leukemia T lymphoblast) whole cell lysate at 20 µg

Lane 4:

Ramos (human Burkitt's lymphoma B lymphocyte) whole cell lysate at 20 µg

Lane 5:

Raji (human Burkitt's lymphoma B lymphocyte) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 37-60 kDa

false

Exposure time: 10s

• Dot

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Dot Blot - Anti-pan cytokeratin antibody [EPR28285-45] (AB308262)

Dot blot analysis of pan cytokeratin using ab308262 at 1 : 1000 dilution (0.541 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 100,000 dilution. Exposure time : 180 seconds. Blocking and diluting buffer and concentration : 5% NFDM/TBST. Lane 1 : Peptides for KRT5, KRT6, KRT7, KRT8 Lane 2 : Peptide for KRT2 Lane 3 : Peptide for KRT1 Lane 4 : Peptide for KRT3 Lane 5 : Peptide for KRT4 Lane 6 : Peptide for KRT23 Lane 7 : Peptides for KRT10, KRT12, KRT15, KRT18, KRT25, KRT26, KRT28 Lane 8 : Peptide for KRT20 Lane 9 : Peptide for KRT9 Lane 10 : Peptides for KRT14, KRT16, KRT17, KRT19, KRT27 Lane 11 : Peptide for KRT24

• WB

Supplier Data

Western blot - Anti-pan cytokeratin antibody [EPR28285-45] (AB308262)

Blocking and diluting buffer and concentration : 5% NFDM/TBST. Low expression : skeletal muscle. In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : Lane 1 : 8 seconds, lanes 2-3 : 15 seconds.

All lanes:

Western blot - Anti-pan cytokeratin antibody [EPR28285-45] (ab308262) at 1/1000 dilution

Lane 1:

Rat colon tissue lysate at 20 µg

Lane 2:

Rat skin tissue lysate at 20 µg

Lane 3:

Rat skeletal muscle tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 37-60 kDa

false