Anti-pan cytokeratin antibody [EPR28285-45] - BSA and Azide free
- RabMAb
- Recombinant
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Rabbit Recombinant Monoclonal K2C1B antibody. Carrier free. Suitable for WB, IHC-P, Dot and reacts with Human, Rat, Mouse, Synthetic peptide samples.
View Alternative Names
KRT1B, KRT77, Cytokeratin-1B, Keratin-77, Type-II keratin Kb39, CK-1B, K77
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan cytokeratin antibody [EPR28285-45] - BSA and Azide free (AB308263)
This data was produced using ab308262, the same clone but in a different formulation. Immunohistochemical analysis of paraffin-embedded human liver tissue labeling pan cytokeratin with ab308262 at 1/5000 dilution (0.108 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on human liver. The section was incubated with ab308262 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan cytokeratin antibody [EPR28285-45] - BSA and Azide free (AB308263)
This data was produced using ab308262, the same clone but in a different formulation. Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma tissue labeling pan cytokeratin with ab308262 at 1/5000 dilution (0.108 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on human adenocarcinoma of colon. The section was incubated with ab308262 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan cytokeratin antibody [EPR28285-45] - BSA and Azide free (AB308263)
This data was produced using ab308262, the same clone but in a different formulation. Immunohistochemical analysis of paraffin-embedded cell pellets labeling pan cytokeratin with ab308262 at 1/5000 dilution (0.108 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Cytoplasmic staining on (A) HeLa cell pellet. No staining on (B) Raji cell pellet. The section was incubated with ab308262 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan cytokeratin antibody [EPR28285-45] - BSA and Azide free (AB308263)
This data was produced using ab308262, the same clone but in a different formulation. Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue labeling pan cytokeratin with ab308262 at 1/5000 dilution (0.108 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Negative control : No staining on human skeletal muscle. The section was incubated with ab308262 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan cytokeratin antibody [EPR28285-45] - BSA and Azide free (AB308263)
This data was produced using ab308262, the same clone but in a different formulation. Immunohistochemical analysis of paraffin-embedded human skin tissue labeling pan cytokeratin with ab308262 at 1/5000 dilution (0.108 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on human skin (PMID : 25411189). The section was incubated with ab308262 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan cytokeratin antibody [EPR28285-45] - BSA and Azide free (AB308263)
This data was produced using ab308262, the same clone but in a different formulation. Immunohistochemical analysis of paraffin-embedded human colon tissue labeling pan cytokeratin with ab308262 at 1/5000 dilution (0.108 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on human colon. The section was incubated with ab308262 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan cytokeratin antibody [EPR28285-45] - BSA and Azide free (AB308263)
This data was produced using ab308262, the same clone but in a different formulation. Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue labeling pan cytokeratin with ab308262 at 1/5000 dilution (0.108 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Negative control : No staining on rat skeletal muscle. The section was incubated with ab308262 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan cytokeratin antibody [EPR28285-45] - BSA and Azide free (AB308263)
This data was produced using ab308262, the same clone but in a different formulation. Immunohistochemical analysis of paraffin-embedded rat skin tissue labeling pan cytokeratin with ab308262 at 1/5000 dilution (0.108 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on rat skin. The section was incubated with ab308262 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan cytokeratin antibody [EPR28285-45] - BSA and Azide free (AB308263)
This data was produced using ab308262, the same clone but in a different formulation. Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue labeling pan cytokeratin with ab308262 at 1/5000 dilution (0.108 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Negative control : No staining on mouse skeletal muscle. The section was incubated with ab308262 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan cytokeratin antibody [EPR28285-45] - BSA and Azide free (AB308263)
This data was produced using ab308262, the same clone but in a different formulation. Immunohistochemical analysis of paraffin-embedded mouse skin tissue labeling pan cytokeratin with ab308262 at 1/5000 dilution (0.108 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on mouse skin. The section was incubated with ab308262 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
- WB
Supplier Data
Western blot - Anti-pan cytokeratin antibody [EPR28285-45] - BSA and Azide free (AB308263)
This data was produced using ab308262, the same clone but in a different formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST. Low expression : skeletal muscle, testis. In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : 10 seconds.
All lanes:
Western blot - Anti-pan cytokeratin antibody [EPR28285-45] (<a href='/en-us/products/primary-antibodies/pan-cytokeratin-antibody-epr28285-45-ab308262'>ab308262</a>) at 1/1000 dilution
Lane 1:
Human liver tissue lysate at 20 µg
Lane 2:
Human colon tissue lysate at 20 µg
Lane 3:
Human skeletal muscle tissue lysate at 20 µg
Lane 4:
Human testis tissue lysate at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution
Observed band size: 37-65 kDa
false
Exposure time: 10s
- WB
Supplier Data
Western blot - Anti-pan cytokeratin antibody [EPR28285-45] - BSA and Azide free (AB308263)
This data was produced using ab308262, the same clone but in a different formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST. Low expression : MOLT-4, Ramos, Raji. In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : 10 seconds.
All lanes:
Western blot - Anti-pan cytokeratin antibody [EPR28285-45] (<a href='/en-us/products/primary-antibodies/pan-cytokeratin-antibody-epr28285-45-ab308262'>ab308262</a>) at 1/1000 dilution
Lane 1:
A431 (human epidermoid carcinoma epithelial cell) whole cell lysate at 20 µg
Lane 2:
HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 3:
MOLT-4 (human lymphoblastic leukemia T lymphoblast) whole cell lysate at 20 µg
Lane 4:
Ramos (human Burkitt's lymphoma B lymphocyte) whole cell lysate at 20 µg
Lane 5:
Raji (human Burkitt's lymphoma B lymphocyte) whole cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution
Observed band size: 37-60 kDa
false
Exposure time: 10s
- Dot
Supplier Data
Dot Blot - Anti-pan cytokeratin antibody [EPR28285-45] - BSA and Azide free (AB308263)
This data was produced using ab308262, the same clone but in a different formulation. Dot blot analysis of pan cytokeratin using ab308262 at 1 : 1000 dilution (0.541 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 100,000 dilution. Exposure time : 180 seconds. Blocking and diluting buffer and concentration : 5% NFDM/TBST. Lane 1 : Peptides for KRT5, KRT6, KRT7, KRT8 Lane 2 : Peptide for KRT2 Lane 3 : Peptide for KRT1 Lane 4 : Peptide for KRT3 Lane 5 : Peptide for KRT4 Lane 6 : Peptide for KRT23 Lane 7 : Peptides for KRT10, KRT12, KRT15, KRT18, KRT25, KRT26, KRT28 Lane 8 : Peptide for KRT20 Lane 9 : Peptide for KRT9 Lane 10 : Peptides for KRT14, KRT16, KRT17, KRT19, KRT27 Lane 11 : Peptide for KRT24
- WB
Supplier Data
Western blot - Anti-pan cytokeratin antibody [EPR28285-45] - BSA and Azide free (AB308263)
This data was produced using ab308262, the same clone but in a different formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST. Low expression : skeletal muscle. In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : Lane 1 : 8 seconds, lanes 2-3 : 15 seconds.
All lanes:
Western blot - Anti-pan cytokeratin antibody [EPR28285-45] (<a href='/en-us/products/primary-antibodies/pan-cytokeratin-antibody-epr28285-45-ab308262'>ab308262</a>) at 1/1000 dilution
Lane 1:
Rat colon tissue lysate at 20 µg
Lane 2:
Rat skin tissue lysate at 20 µg
Lane 3:
Rat skeletal muscle tissue lysate at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution
Observed band size: 37-60 kDa
false
Reactivity data
Product details
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Properties and storage information
Form
Purification technique
Storage buffer
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Keratins in pan cytokeratin contribute to maintaining structural stability within epithelial cells. They act as components of the cytoskeletal network interacting with other cellular structures to provide protection from stress and mechanical damage. Pan cytokeratin exists as part of the intermediate filament protein complex which plays a significant role in cellular mechanics and cohesion essential for tissue integrity.
Pathways
Intermediate filaments containing pan cytokeratin participate in pathways related to cell differentiation and stress response. Its interactions with other proteins like desmoplakin and plectin link the cytoskeletal filaments to cell membranes and the extracellular matrix. This involvement is important in pathways related to cellular structural integrity and epithelial-mesenchymal transition (EMT).
Product protocols
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Target data
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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