AB234297

Anti-pan Cytokeratin antibody [KRT/1877R]

Name: Anti-pan Cytokeratin antibody [KRT/1877R] (ab234297) | Abcam
Brand: Abcam Limited
SKU: AB234297
Price: 400 USD
Availability: InStock
Rating: 4 (6 reviews)

Lab Essentials
What is this?

(6 Reviews)

(26 Publications)

Anti-pan Cytokeratin antibody [KRT/1877R] (ab234297) is a rabbit monoclonal antibody detecting pan Cytokeratin in IHC-P. Suitable for Human.

View Alternative Names

pan-ck

2 Images

Supplier Data

Immunocytochemistry - Anti-pan Cytokeratin antibody [KRT/1877R] (AB234297)

Immunocytochemistry analysis of MCF7 cells labeling pan Cytokeratin with ab234297 at 2µg/mL followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cytokeratin antibody [KRT/1877R] (AB234297)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cytokeratin antibody [KRT/1877R] (AB234297)

Formalin-fixed, paraffin-embedded human skin tissue stained for pan Cytokeratin using ab234297 at 1 μg/ml in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

KRT/1877R

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "0.5-1 µg/mL", "IHCP-species-notes": "<p>Primary incubation for 30 minutes at room temperature.</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1-2 µg/mL", "ICC-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "" }, "Goat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "" }, "Guinea pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "" }, "Monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "" } } }

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Product details

What is this antibody validated in?
Anti-pan Cytokeratin antibody [KRT/1877R] (ab234297) is a rabbit monoclonal antibody and is validated for use in Immunohistochemistry (IHC-P) in Human samples.

Reviewed by scientists
Anti-pan Cytokeratin [KRT/1877R] (ab234297) has over 5 independent reviews from customers.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A/G

Purification notes

Purified from Bioreactor Concentrate by Protein A/G.

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Pan cytokeratin also known as cytokeratin AE1/AE3 is a cytoskeletal protein complex that consists of different keratin polypeptides. It mechanically supports epithelial cells contributing to their structural integrity. The protein mass is variable due to its composite nature ranging between 40 to 67 kDa. Pan cytokeratin is expressed in epithelial tissues throughout the body serving as a reliable marker for identifying epithelial origin cells in histological studies.

Biological function summary

Keratins in pan cytokeratin contribute to maintaining structural stability within epithelial cells. They act as components of the cytoskeletal network interacting with other cellular structures to provide protection from stress and mechanical damage. Pan cytokeratin exists as part of the intermediate filament protein complex which plays a significant role in cellular mechanics and cohesion essential for tissue integrity.

Pathways

Intermediate filaments containing pan cytokeratin participate in pathways related to cell differentiation and stress response. Its interactions with other proteins like desmoplakin and plectin link the cytoskeletal filaments to cell membranes and the extracellular matrix. This involvement is important in pathways related to cellular structural integrity and epithelial-mesenchymal transition (EMT).

Pan cytokeratin serves as a critical marker in the diagnosis of epithelial-derived cancers such as carcinoma and conditions like psoriasis. Abnormal expression patterns of pan cytokeratin indicate tumor presence and origin. Its connection to proteins such as E-cadherin and vimentin assists in understanding the progression and metastasis of cancer by facilitating EMT and tissue invasion diagnostics.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information pan Cytokeratin

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Publications (26)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 26: PubMed40565011

2025

Bioprinted Four-Cell-Type Lung Model for Viral Infection Studies Under Air-Liquid Interface Conditions.

Applications

Unspecified application

Species

Unspecified reactive species

Johanna Berg,Julian Heinze,Daniela Niemeyer,Josefin Hellgren,Himjyot Jaiswal,Anna Löwa,Andreas Hocke,Itedale Namro,Christian Drosten,Jens Kurreck,Beatrice Tolksdorf

ACS omega 10:23121-23128 PubMed40521560

2025

Setup of an In Vitro Three-Dimensional Stromalized Prostate Cancer Model Using Gelatin Microparticles.

Applications

Unspecified application

Species

Unspecified reactive species

Giulia Gangarossa,Marta Iozzo,Giulia Mugnaini,Rita Gelli,Luigi Ippolito,Elisa Giannoni,Giuseppina Comito,Massimo Bonini,Paola Chiarugi

Journal of experimental & clinical cancer research : CR 44:175 PubMed40518539

2025

PARP-1 as a novel target in endocrine-resistant breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Azzurra Zicarelli,Marianna Talia,Muriel Lainé,Rosamaria Lappano,Marcello Maggiolini,Geoffrey L Greene

Scientific reports 15:19759 PubMed40473768

2025

Development of a two-layer 3D equine endometrial tissue model using genipin-crosslinked collagen scaffolds and 3D printing.

Applications

Unspecified application

Species

Unspecified reactive species

Sawita Santiviparat,Setthibhak Suthithanakom,Sudchaya Bhanpattanakul,Sayamon Srisuwattanasagul,Kai Melde,Tom A E Stout,Theerawat Tharasanit

Communications biology 8:621 PubMed40240529

2025

Breast cancer patient-derived scaffolds enhance the understanding of PD-L1 regulation and T cell cytotoxicity.

Applications

Unspecified application

Species

Unspecified reactive species

Elena Garre,Sara Rhost,Anna Gustafsson,Louis Szeponik,Thais Fenz Araujo,Marianne Quiding-Järbrink,Khalil Helou,Anders Ståhlberg,Göran Landberg

Nature methods 22:962-972 PubMed40128288

2025

Single-cell parallel analysis of DNA damage and transcriptome reveals selective genome vulnerability.

Applications

Unspecified application

Species

Unspecified reactive species

Dongsheng Bai,Zhenkun Cao,Nivedita Attada,Jinghui Song,Chenxu Zhu

Signal transduction and targeted therapy 9:342 PubMed39638994

2024

CXCR1 neutrophil infiltration orchestrates response to third-generation EGFR-TKI in EGFR mutant non-small-cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Haowei Wang,Anwen Xiong,Xiaoxia Chen,Junhong Guo,Zhuoran Tang,Chunyan Wu,Shengxiang Ren,Caicun Zhou,Jian Chen,Likun Hou,Tao Jiang

Cell death & disease 15:797 PubMed39505867

2024

DKK1+ tumor cells inhibited the infiltration of CCL19+ fibroblasts and plasma cells contributing to worse immunotherapy response in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Guangyu Fan,Ruyun Gao,Tongji Xie,Lin Li,Le Tang,Xiaohong Han,Yuankai Shi

Journal of translational medicine 22:917 PubMed39385235

2024

Spatial analyses revealed S100P + TFF1 + tumor cells in spread through air spaces samples correlated with undesirable therapy response in non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Guangyu Fan,Tongji Xie,Mengwei Yang,Lin Li,Le Tang,Xiaohong Han,Yuankai Shi

Clinical and translational medicine 14:e70009 PubMed39187937

2024

The co-location of CD14+APOE+ cells and MMP7+ tumour cells contributed to worse immunotherapy response in non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Guangyu Fan,Tongji Xie,Le Tang,Lin Li,Xiaohong Han,Yuankai Shi

View all publications

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