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AB215838

Anti-pan Cytokeratin antibody [PAN-CK (Cocktail)]

2

(2 Reviews)

|

(38 Publications)

Mouse Monoclonal Cytokeratin 2P antibody. Suitable for ICC, Flow Cyt, IHC-P and reacts with Human samples. Cited in 38 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human KRT76.

View Alternative Names

KRT2B, KRT2P, KRT76, Cytokeratin-2P, Keratin-76, Type-II keratin Kb9, CK-2P, K2P, K76, KRT1B, KRT77, Cytokeratin-1B, Keratin-77, Type-II keratin Kb39, CK-1B, K77

4 Images
Flow Cytometry - Anti-pan Cytokeratin antibody [PAN-CK (Cocktail)] (AB215838)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-pan Cytokeratin antibody [PAN-CK (Cocktail)] (AB215838)

Flow cytometry analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling pan-Cytokeratin with ab215838 at 2µg/10^6 cells followed by Goat Anti-Mouse IgG-CF488 (blue); isotype control (red).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cytokeratin antibody [PAN-CK (Cocktail)] (AB215838)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cytokeratin antibody [PAN-CK (Cocktail)] (AB215838)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human basal cell carcinoma tissue labelling pan Cytokeratin with ab215838 at 2 μg/mL for 30 minutes at room temperature. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at room temperature for 20 minutes.

Immunocytochemistry - Anti-pan Cytokeratin antibody [PAN-CK (Cocktail)] (AB215838)
  • ICC

Supplier Data

Immunocytochemistry - Anti-pan Cytokeratin antibody [PAN-CK (Cocktail)] (AB215838)

Immunofluorescence analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling pan Cytokeratin with ab215838 at 2 µg/mL followed by Goat anti-Mouse IgG-CF488 (cyan). The nuclear counterstain is NucSpot (red).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cytokeratin antibody [PAN-CK (Cocktail)] (AB215838)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cytokeratin antibody [PAN-CK (Cocktail)] (AB215838)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human squamous cell carcinoma tissue labelling pan Cytokeratin with ab215838 at 2 μg/mL for 30 minutes at room temperature. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at room temperature for 20 minutes.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

PAN-CK (Cocktail)

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC, Flow Cyt, IHC-P

applications

Immunogen

Native Full Length Protein corresponding to Human KRT76.

Q01546

Specificity

This antibody cocktail recognizes acidic (Type I or LMW) and basic (Type II or HMW) Cytokeratins, with 67kDa (Cytokeratin1); 64kDa (Cytokeratin3); 59kDa (Cytokeratin4); 58kDa (Cytokeratin5); 56kDa (Cytokeratin6); 55kDa (Cytokeratin7); 52kDa (Cytokeratin8); 56.5kDa (Cytokeratin10); 53kDa (Cytokeratin13); 50kDa (Cytokeratin14); 50kDa (Cytokeratin15); 48kDa (Cytokeratin16); 46kDa (Cytokeratin17); 45kDa (Cytokeratin18) and 40kDa (Cytokeratin19).

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1-2 µg/mL", "ICC-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.5-2 µg for 10^6 Cells", "FlowCyt-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chicken": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Monkey": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rabbit": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

Isotype is mouse IgGs, kappa.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Purification notes
ab215838 is purified from Bioreactor Concentrate by Protein A/G.
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Pan cytokeratin also known as cytokeratin AE1/AE3 is a cytoskeletal protein complex that consists of different keratin polypeptides. It mechanically supports epithelial cells contributing to their structural integrity. The protein mass is variable due to its composite nature ranging between 40 to 67 kDa. Pan cytokeratin is expressed in epithelial tissues throughout the body serving as a reliable marker for identifying epithelial origin cells in histological studies.
Biological function summary

Keratins in pan cytokeratin contribute to maintaining structural stability within epithelial cells. They act as components of the cytoskeletal network interacting with other cellular structures to provide protection from stress and mechanical damage. Pan cytokeratin exists as part of the intermediate filament protein complex which plays a significant role in cellular mechanics and cohesion essential for tissue integrity.

Pathways

Intermediate filaments containing pan cytokeratin participate in pathways related to cell differentiation and stress response. Its interactions with other proteins like desmoplakin and plectin link the cytoskeletal filaments to cell membranes and the extracellular matrix. This involvement is important in pathways related to cellular structural integrity and epithelial-mesenchymal transition (EMT).

Pan cytokeratin serves as a critical marker in the diagnosis of epithelial-derived cancers such as carcinoma and conditions like psoriasis. Abnormal expression patterns of pan cytokeratin indicate tumor presence and origin. Its connection to proteins such as E-cadherin and vimentin assists in understanding the progression and metastasis of cancer by facilitating EMT and tissue invasion diagnostics.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Probably contributes to terminal cornification.
See full target information KRT76

Additional targets

KRT77
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Publications (38)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 16:1596434 PubMed40771810

2025

Pygo2+ T cells possess immunosuppressive features and inferior immunotherapeutic response in gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Weilong Chang,Huifang Yan,Yawei Zhang,Zibo Sang,Bei Bu,Rui Deng,Kaibo Li,Jiajing Li,Yang Fu,Jinyuan Cui

iScience 27:111169 PubMed39555401

2024

ZEB2 drives intra-tumor heterogeneity and skin squamous cell carcinoma formation with distinct EMP transition states.

Applications

Unspecified application

Species

Unspecified reactive species

Jeroen Verstappe,Nicolas Skrypek,Jordy De Coninck,Bieke Soen,Joachim Taminau,Marianthi Tatari,Kenneth Bruneel,Nele Loret,Kato De Clercq,Caroline Van den Broecke,Koen Van De Vijver,Jo Van Dorpe,Jody Haigh,Bram De Craene,Steven Goossens,Niels Vandamme,Geert Berx

Medicine 103:e39892 PubMed39465830

2024

LEF1 is associated with immunosuppressive microenvironment of patients with lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoqing Liu,Chunlou Wang,Xiaoling Zhang,Rongju Zhang

Journal of inflammation research 17:7261-7274 PubMed39429850

2024

Activating Transcription Factor 6 Mediates Inflammation in Experimental Varicocele-Induced Epididymal Epithelial Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yin-Shan Jin,Yuan-Qing Cui,Yan-Ping Xu,Jie Chen,Xue-Bao Zhang,Xiong Wang

Cell reports. Medicine 5:101757 PubMed39368483

2024

Clinical-proteomic classification and precision treatment strategy of chordoma.

Applications

Unspecified application

Species

Unspecified reactive species

Huabin Yin,Jinbo Hu,Jianxuan Gao,Tong Su,Jiali Jin,Cong Jiang,Wenxuan Yin,Xiaowen Xu,Zhengyan Chang,Wei Sun,Zhengdong Cai,Wang Zhou,Ping Wang,Jun Lin,Dianwen Song,Tong Meng

Neoplasia (New York, N.Y.) 57:101060 PubMed39357265

2024

Distinction of papillary and adamantinomatous craniopharyngioma: Clinical features, surgical nuances and hypothalamic outcomes.

Applications

Unspecified application

Species

Unspecified reactive species

Le Yang,Yi Liu,ChaoHu Wang,ZhanPeng Feng,Lei Yu,Jun Pan,JunXiang Peng,Jing Nie,MingFeng Zhou,YiChao Ou,Tao Liu,Songtao Qi,Jun Fan

Frontiers in pharmacology 15:1466779 PubMed39351094

2024

Integrated analyses reveal IDO1 as a prognostic biomarker coexpressed with PD-1 on tumor-associated macrophages in esophageal squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yaojun Peng,Lingxiong Wang,Juan Yang,Qiyan Wu,Xiaoxuan Sun,Jinying Zhang,Yanju Yu,Liping Zhang,Jie Gao,Qing Zhou,Haiyan Zhu,Fan Yin

Journal of experimental & clinical cancer research : CR 43:210 PubMed39075504

2024

Pericytes recruited by CCL28 promote vascular normalization after anti-angiogenesis therapy through RA/RXRA/ANGPT1 pathway in lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Chen,Zhiyong Zhang,Fan Pan,Pengfei Li,Weiping Yao,Yuxi Chen,Lei Xiong,Tingting Wang,Yan Li,Guichun Huang

Annals of medicine and surgery (2012) 86:2644-2650 PubMed38694326

2024

S100A6 could not promote the differentiation of Calu-6 lung cancer cell line.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Li,Ting Wang,Dan Dang

Oncology letters 27:131 PubMed38362233

2024

Prognosis value of circulating tumor cell PD‑L1 and baseline characteristics in patients with NSCLC treated with immune checkpoint inhibitors plus platinum‑containing drugs.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaona Su,Ci Zhou,Shu Chen,Qiang Ma,He Xiao,Qian Chen,Hua Zou
View all publications
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