Mouse Monoclonal Cytokeratin 2P antibody. Suitable for ICC, Flow Cyt, IHC-P and reacts with Human samples. Cited in 26 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human KRT76.
Images
![Immunocytochemistry - Anti-pan Cytokeratin antibody [PAN-CK (Cocktail)] (AB215838), expandable thumbnail](https://content.abcam.com/products/images/pan-cytokeratin-antibody-pan-ck-cocktail-ab215838--immunocytochemistry-img80220.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry - Anti-pan Cytokeratin antibody [PAN-CK (Cocktail)] (AB215838)")
![Flow Cytometry - Anti-pan Cytokeratin antibody [PAN-CK (Cocktail)] (AB215838), expandable thumbnail](https://content.abcam.com/products/images/pan-cytokeratin-antibody-pan-ck-cocktail-ab215838--flow-cytometry-img66625.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-pan Cytokeratin antibody [PAN-CK (Cocktail)] (AB215838)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cytokeratin antibody [PAN-CK (Cocktail)] (AB215838), expandable thumbnail](https://content.abcam.com/products/images/pan-cytokeratin-antibody-pan-ck-cocktail-ab215838--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img139552.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cytokeratin antibody [PAN-CK (Cocktail)] (AB215838)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cytokeratin antibody [PAN-CK (Cocktail)] (AB215838), expandable thumbnail](https://content.abcam.com/products/images/pan-cytokeratin-antibody-pan-ck-cocktail-ab215838--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img107509.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cytokeratin antibody [PAN-CK (Cocktail)] (AB215838)")
Publications
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- Journal of translational medicine 21:9112023IL20RB signaling enhances stemness and chemotherapy resistance in pancreatic cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiao-Hui Li,Gui-Zhong Huang,Zi-Lan Xu,Chong-Yu Zhao,Xiao-Yuan Dong,Bo-Kang Cui,Xiao-Jun LinPubMed 38098005
- Technology in cancer research & treatment 22:153303382311678272023Enrichment of Circulating Tumor Cells of Lung Cancer and Correlation With Serum Leukomonocyte and Tumor Biomarkers: A Retrospective Study.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhou-Fang Mei,Jing Yan,Ling Qian,Yan-Chao He,Jing-Jing Feng,Jie Wang,Zhi-Jun Jie,Hua ZouPubMed 37078167
- Experimental and therapeutic medicine 25:1322023Sclerosing extramedullary hematopoietic tumor of the colon: A case report and literature review.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhibo Zuo,Qi Zhang,Wanxin Wu,Xin Li,Lin Zhang,Jingyu Wang,Zhiqin Guo,Shaoqing Hu,Hongyan LiuPubMed 36845951
- Journal of medical virology 95:e284802023Single-cell transcriptomics dissects epithelial heterogeneity in HPV cervical adenocarcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCong Wang,Lei Li,Fuhao Wang,Xia Li,Jujie Sun,Xiaohui Li,Tianyu Lei,Qingyu Huang,Guangyu Zhang,Hongqing Wang,Dapeng Li,Jue Jia,Chunyan Li,Feng Geng,Jinbo Yue,Chao LiuPubMed 36609919
- Cancer medicine 12:7283-72932022Comprehensive optimization of urinary exfoliated tumor cells tests in bladder cancer with a promising microfluidic platform.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFengbin Gao,Jie Wang,Yanlan Yu,Jing Yan,Guoqing DingPubMed 36567509
- Cancer 129:393-4042022The clinicopathological significance and relapse predictive role of tumor microenvironment of intrahepatic cholangiocarcinoma after radical surgery.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYiling Zheng,Ning Huang,Shuwen Kuang,Jing Zhang,Hong Zhao,Jianxiong Wu,Mei Liu,Liming WangPubMed 36433731
- EBioMedicine 86:1043712022Impacts of neoadjuvant chemoradiotherapy on the immune landscape of esophageal squamous cell carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJing Wen,Shuogui Fang,Yi Hu,Mian Xi,Zelin Weng,Chuqing Pan,Kongjia Luo,Yihong Ling,Renchun Lai,Xiuying Xie,Xiaodan Lin,Ting Lin,Jiyang Chen,Qianwen Liu,Jianhua Fu,Hong YangPubMed 36434949
- Bosnian journal of basic medical sciences 22:707-7152022The expression of VISTA on CD4+ T cells associate with poor prognosis and immune status in non-small cell lung cancer patients.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShengyao Ma,Liya Qin,Xinling Wang,Weiyu Wang,Jinfeng Li,Huaijie Wang,Hanyue Li,Xiaoshan Cai,Yang Yang,Meihua QuPubMed 35122478
- Advanced healthcare materials 11:e22016802022An Acellular Scaffold Facilitates Endometrial Regeneration and Fertility Restoration via Recruiting Endogenous Mesenchymal Stem Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLiaobing Xin,Xiaowen Zheng,Jianmin Chen,Sentao Hu,Yilun Luo,Qunzi Ge,Xiaoying Jin,Lie Ma,Songying ZhangPubMed 36049781
- BMJ open 12:e0540092022Potential key roles of tumour budding: a representative malignant pathological feature of non-small cell lung cancer and a sensitive indicator of prognosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLi Qian,Jianguo Zhang,Shumin Lu,Xin He,Jia Feng,Jiahai Shi,Yifei LiuPubMed 35361643
Key facts
- Isotype
- IgG
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Native Full Length Protein corresponding to Human KRT76. Database link Q01546
Reactivity data
Select an application| ICC | Flow Cyt | IHC-P | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Predicted | Predicted | Predicted |
| Rat | Predicted | Predicted | Predicted |
| Chicken | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted |
| Dog | Predicted | Predicted | Predicted |
| Monkey | Predicted | Predicted | Predicted |
| Rabbit | Predicted | Predicted | Predicted |
ICC
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1-2 µg/mL | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Rabbit, Chicken, Cow, Dog, Monkey | Dilution info - | Notes - |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.5-2 µg for 106 Cells | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Rabbit, Chicken, Cow, Dog, Monkey | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1-2 µg/mL | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Rabbit, Chicken, Cow, Dog, Monkey | Dilution info - | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
1 product for Alternative Version
Additional Targets
KRT77
Alternative names
KRT2B, KRT2P, KRT76, Cytokeratin-2P, Keratin-76, Type-II keratin Kb9, CK-2P, K2P, K76
Recommended products
Mouse Monoclonal Cytokeratin 2P antibody. Suitable for ICC, Flow Cyt, IHC-P and reacts with Human samples. Cited in 26 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human KRT76.
Key facts
- Isotype
- IgG
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Native Full Length Protein corresponding to Human KRT76. Database link Q01546
- Clone number
- PAN-CK (Cocktail)
- Purification technique
- Affinity purification Protein A/G
- Specificity
This antibody cocktail recognizes acidic (Type I or LMW) and basic (Type II or HMW) Cytokeratins, with 67kDa (Cytokeratin1); 64kDa (Cytokeratin3); 59kDa (Cytokeratin4); 58kDa (Cytokeratin5); 56kDa (Cytokeratin6); 55kDa (Cytokeratin7); 52kDa (Cytokeratin8); 56.5kDa (Cytokeratin10); 53kDa (Cytokeratin13); 50kDa (Cytokeratin14); 50kDa (Cytokeratin15); 48kDa (Cytokeratin16); 46kDa (Cytokeratin17); 45kDa (Cytokeratin18) and 40kDa (Cytokeratin19).
- Concentration
- Purification notes
ab215838 is purified from Bioreactor Concentrate by Protein A/G.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Isotype is mouse IgGs, kappa.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Pan cytokeratin also known as cytokeratin AE1/AE3 is a cytoskeletal protein complex that consists of different keratin polypeptides. It mechanically supports epithelial cells contributing to their structural integrity. The protein mass is variable due to its composite nature ranging between 40 to 67 kDa. Pan cytokeratin is expressed in epithelial tissues throughout the body serving as a reliable marker for identifying epithelial origin cells in histological studies.
- Biological function summary
Keratins in pan cytokeratin contribute to maintaining structural stability within epithelial cells. They act as components of the cytoskeletal network interacting with other cellular structures to provide protection from stress and mechanical damage. Pan cytokeratin exists as part of the intermediate filament protein complex which plays a significant role in cellular mechanics and cohesion essential for tissue integrity.
- Pathways
Intermediate filaments containing pan cytokeratin participate in pathways related to cell differentiation and stress response. Its interactions with other proteins like desmoplakin and plectin link the cytoskeletal filaments to cell membranes and the extracellular matrix. This involvement is important in pathways related to cellular structural integrity and epithelial-mesenchymal transition (EMT).
- Associated diseases and disorders
Pan cytokeratin serves as a critical marker in the diagnosis of epithelial-derived cancers such as carcinoma and conditions like psoriasis. Abnormal expression patterns of pan cytokeratin indicate tumor presence and origin. Its connection to proteins such as E-cadherin and vimentin assists in understanding the progression and metastasis of cancer by facilitating EMT and tissue invasion diagnostics.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Immunocytochemistry - Anti-pan Cytokeratin antibody [PAN-CK (Cocktail)] (ab215838), expandable thumbnail](https://content.abcam.com/products/images/pan-cytokeratin-antibody-pan-ck-cocktail-ab215838--immunocytochemistry-img80220.jpg "Immunocytochemistry - Anti-pan Cytokeratin antibody [PAN-CK (Cocktail)] (ab215838)")
Immunocytochemistry - Anti-pan Cytokeratin antibody [PAN-CK (Cocktail)] (ab215838)
Immunofluorescence analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling pan Cytokeratin with ab215838 at 2 µg/mL followed by Goat anti-Mouse IgG-CF488 (cyan). The nuclear counterstain is NucSpot (red).
![Flow Cytometry - Anti-pan Cytokeratin antibody [PAN-CK (Cocktail)] (ab215838), expandable thumbnail](https://content.abcam.com/products/images/pan-cytokeratin-antibody-pan-ck-cocktail-ab215838--flow-cytometry-img66625.jpg "Flow Cytometry - Anti-pan Cytokeratin antibody [PAN-CK (Cocktail)] (ab215838)")
Flow Cytometry - Anti-pan Cytokeratin antibody [PAN-CK (Cocktail)] (ab215838)
Flow cytometry analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling pan-Cytokeratin with ab215838 at 2µg/10^6 cells followed by Goat Anti-Mouse IgG-CF488 (blue); isotype control (red).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cytokeratin antibody [PAN-CK (Cocktail)] (ab215838), expandable thumbnail](https://content.abcam.com/products/images/pan-cytokeratin-antibody-pan-ck-cocktail-ab215838--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img139552.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cytokeratin antibody [PAN-CK (Cocktail)] (ab215838)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cytokeratin antibody [PAN-CK (Cocktail)] (ab215838)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human basal cell carcinoma tissue labelling pan Cytokeratin with ab215838 at 2 μg/mL for 30 minutes at room temperature. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at room temperature for 20 minutes.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cytokeratin antibody [PAN-CK (Cocktail)] (ab215838), expandable thumbnail](https://content.abcam.com/products/images/pan-cytokeratin-antibody-pan-ck-cocktail-ab215838--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img107509.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cytokeratin antibody [PAN-CK (Cocktail)] (ab215838)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cytokeratin antibody [PAN-CK (Cocktail)] (ab215838)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human squamous cell carcinoma tissue labelling pan Cytokeratin with ab215838 at 2 μg/mL for 30 minutes at room temperature. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at room temperature for 20 minutes.
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