AB6401

Anti-pan Cytokeratin antibody [PCK-26]

Name: Anti-pan Cytokeratin antibody [PCK-26] (ab6401) | Abcam
Brand: Abcam Limited
SKU: AB6401
Rating: 4 (20 reviews)

Lab Essentials
What is this?

(20 Reviews)

(53 Publications)

Mouse Monoclonal Cytokeratin 2P antibody. Suitable for WB and reacts with Human samples. Cited in 53 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human KRT76.

View Alternative Names

KRT2B, KRT2P, KRT76, Cytokeratin-2P, Keratin-76, Type-II keratin Kb9, CK-2P, K2P, K76

2 Images

Supplier Data

Western blot - Anti-pan Cytokeratin antibody [PCK-26] (AB6401)

This image was generated using the ascites version of the product.

Lane 1:

Western blot - Anti-pan Cytokeratin antibody [PCK-26] (ab6401) at 2.5 µg/mL

Lane 2:

Western blot - Anti-pan Cytokeratin antibody [PCK-26] (ab6401) at 5 µg/mL

Lane 3:

Western blot - Anti-pan Cytokeratin antibody [PCK-26] (ab6401) at 10 µg/mL

All lanes:

HeLa cell lysate

Secondary

All lanes:

Peroxidase-conjugated goat anti-mouse IgG

false

AbReview19168****

Western blot - Anti-pan Cytokeratin antibody [PCK-26] (AB6401)

All lanes : pan Cytokeratin antibody (ab6401) at 1/1000 dilution + whole cell lysate of Primary mouse pancreatic cancer cell lines (35 μg)

Secondary : An HRP-conjugated Sheep anti-mouse IgG polyclonal (1/5000) developed using the ECL technique

Performed under non-reducing conditions.

Blocking Step : 10% Milk for 1 hour at room temperature

This image was generated using the ascites version of the product.

All lanes:

Western blot - Anti-pan Cytokeratin antibody [PCK-26] (ab6401)

Observed band size: 55 kDa

false

This image is courtesy of an Abreview submitted by Pawel Mazur

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

PCK-26

Isotype

IgG1

Light chain type

unknown

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Native Full Length Protein corresponding to Human KRT76.

Q01546

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Goat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

This product was changed from ascites to tissue culture supernatant on 17 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Purification notes

Purified from TCS.

Storage buffer

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Pan cytokeratin also known as cytokeratin AE1/AE3 is a cytoskeletal protein complex that consists of different keratin polypeptides. It mechanically supports epithelial cells contributing to their structural integrity. The protein mass is variable due to its composite nature ranging between 40 to 67 kDa. Pan cytokeratin is expressed in epithelial tissues throughout the body serving as a reliable marker for identifying epithelial origin cells in histological studies.

Biological function summary

Keratins in pan cytokeratin contribute to maintaining structural stability within epithelial cells. They act as components of the cytoskeletal network interacting with other cellular structures to provide protection from stress and mechanical damage. Pan cytokeratin exists as part of the intermediate filament protein complex which plays a significant role in cellular mechanics and cohesion essential for tissue integrity.

Pathways

Intermediate filaments containing pan cytokeratin participate in pathways related to cell differentiation and stress response. Its interactions with other proteins like desmoplakin and plectin link the cytoskeletal filaments to cell membranes and the extracellular matrix. This involvement is important in pathways related to cellular structural integrity and epithelial-mesenchymal transition (EMT).

Pan cytokeratin serves as a critical marker in the diagnosis of epithelial-derived cancers such as carcinoma and conditions like psoriasis. Abnormal expression patterns of pan cytokeratin indicate tumor presence and origin. Its connection to proteins such as E-cadherin and vimentin assists in understanding the progression and metastasis of cancer by facilitating EMT and tissue invasion diagnostics.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Probably contributes to terminal cornification.

See full target information KRT76

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Publications (53)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:10534 PubMed39627211

2024

Differential activity of MAPK signalling defines fibroblast subtypes in pancreatic cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Lisa Veghini,Davide Pasini,Rui Fang,Pietro Delfino,Dea Filippini,Christian Neander,Caterina Vicentini,Elena Fiorini,Francesca Lupo,Sabrina L D'Agosto,Carmine Carbone,Antonio Agostini,Geny Piro,Diego Rosa,Michele Bevere,Peter Markus,Diana Behrens,Claudio Luchini,Rita T Lawlor,Aldo Scarpa,Giulia Biffi,Phyllis F Cheung,Jens T Siveke,Vincenzo Corbo

Molecular oncology 19:357-376 PubMed39478658

2024

Minimally invasive determination of pancreatic ductal adenocarcinoma (PDAC) subtype by means of circulating cell-free RNA.

Applications

Unspecified application

Species

Unspecified reactive species

Martin Metzenmacher,Gregor Zaun,Marija Trajkovic-Arsic,Phyllis Cheung,Timm M Reissig,Hendrik Schürmann,Nils von Neuhoff,Grainne O'Kane,Stephanie Ramotar,Anna Dodd,Steven Gallinger,Alexander Muckenhuber,Jennifer J Knox,Volker Kunzmann,Peter A Horn,Jörg D Hoheisel,Jens T Siveke,Smiths S Lueong

Current issues in molecular biology 46:1799-1809 PubMed38534734

2024

A Potential Role of CD82/KAI1 during Uterine Decidualization in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Qijun Li,Mengyao Song,Ke Cao,Qian Zhang

Cell reports. Medicine 4:101330 PubMed38118415

2023

Needle biopsy accelerates pro-metastatic changes and systemic dissemination in breast cancer: Implications for mortality by surgery delay.

Applications

Unspecified application

Species

Unspecified reactive species

Hiroyasu Kameyama,Priya Dondapati,Reese Simmons,Macall Leslie,John F Langenheim,Yunguang Sun,Misung Yi,Aubrey Rottschaefer,Rashmi Pathak,Shreya Nuguri,Kar-Ming Fung,Shirng-Wern Tsaih,Inna Chervoneva,Hallgeir Rui,Takemi Tanaka

Science advances 9:eadg5109 PubMed37831776

2023

Coinhibition of topoisomerase 1 and BRD4-mediated pause release selectively kills pancreatic cancer via readthrough transcription.

Applications

Unspecified application

Species

Unspecified reactive species

Donald P Cameron,Jan Grosser,Swetlana Ladigan,Vladislav Kuzin,Evanthia Iliopoulou,Anika Wiegard,Hajar Benredjem,Kathryn Jackson,Sven T Liffers,Smiths Lueong,Phyllis F Cheung,Deepak Vangala,Michael Pohl,Richard Viebahn,Christian Teschendorf,Heiner Wolters,Selami Usta,Keyi Geng,Claudia Kutter,Marie Arsenian-Henriksson,Jens T Siveke,Andrea Tannapfel,Wolff Schmiegel,Stephan A Hahn,Laura Baranello

American journal of physiology. Lung cellular and molecular physiology 325:L447-L459 PubMed37529852

2023

Complex urban atmosphere alters alveolar stem cells niche properties and drives lung fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Randa Belgacemi,Bruno Ribeiro Baptista,Grégoire Justeau,Marylène Toigo,Andrew Frauenpreis,Rojda Yilmaz,Audrey Der Vartanian,Mathieu Cazaunau,Edouard Pangui,Antonin Bergé,Aline Gratien,Juan Camilo Macias Rodriguez,Saverio Bellusci,Geneviève Derumeaux,Jorge Boczkowski,Denise Al Alam,Patrice Coll,Sophie Lanone,Laurent Boyer

Oncogenesis 12:23 PubMed37130839

2023

Retinoic acid receptor β modulates mechanosensing and invasion in pancreatic cancer cells via myosin light chain 2.

Applications

Unspecified application

Species

Unspecified reactive species

Carlos Matellan,Dariusz Lachowski,Ernesto Cortes,Kai Ning Chiam,Aleksandar Krstic,Stephen D Thorpe,Armando E Del Río Hernández

Cancer & metabolism 10:24 PubMed36494842

2022

Functional noninvasive detection of glycolytic pancreatic ductal adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Irina Heid,Corinna Münch,Sinan Karakaya,Smiths S Lueong,Alina M Winkelkotte,Sven T Liffers,Laura Godfrey,Phyllis F Y Cheung,Konstantinos Savvatakis,Geoffrey J Topping,Florian Englert,Lukas Kritzner,Martin Grashei,Andrea Tannapfel,Richard Viebahn,Heiner Wolters,Waldemar Uhl,Deepak Vangala,Esther M M Smeets,Erik H J G Aarntzen,Daniel Rauh,Wilko Weichert,Jörg D Hoheisel,Stephan A Hahn,Franz Schilling,Rickmer Braren,Marija Trajkovic-Arsic,Jens T Siveke

Cellular & molecular immunology 19:777-790 PubMed35468944

2022

IFN-γ cytotoxic CD4 T lymphocytes are involved in the pathogenesis of colitis induced by IL-23 and the food colorant Red 40.

Applications

Unspecified application

Species

Unspecified reactive species

Lili Chen,Zhengxiang He,Bernardo S Reis,Jesse D Gelles,Jerry Edward Chipuk,Adrian T Ting,Julie A Spicer,Joseph A Trapani,Glaucia C Furtado,Sergio A Lira

Nature communications 12:5761 PubMed34599177

2021

Intrathymic differentiation of natural antibody-producing plasma cells in human neonates.

Applications

Unspecified application

Species

Unspecified reactive species

Hector Cordero,Rodney G King,Pranay Dogra,Chloe Dufeu,Sarah B See,Alexander M Chong,Anne-Catrin Uhlemann,Siu-Hong Ho,David M Kalfa,Emile A Bacha,John F Kearney,Emmanuel Zorn

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-pan Cytokeratin antibody [PCK-26]

Western blot - Anti-pan Cytokeratin antibody [PCK-26] (AB6401)

Western blot - Anti-pan Cytokeratin antibody [PCK-26] (AB6401)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purity

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (53)

Product promise