Mouse Monoclonal Cytokeratin 2P antibody. Suitable for WB and reacts with Human samples. Cited in 45 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human KRT76.
Images
![Western blot - Anti-pan Cytokeratin antibody [PCK-26] (AB6401), expandable thumbnail](https://content.abcam.com/products/images/pan-cytokeratin-antibody-pck-26-ab6401--western-blot-img12340.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-pan Cytokeratin antibody [PCK-26] (AB6401)")
![Western blot - Anti-pan Cytokeratin antibody [PCK-26] (AB6401), expandable thumbnail](https://content.abcam.com/products/images/pan-cytokeratin-antibody-pck-26-ab6401--western-blot-img97282.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-pan Cytokeratin antibody [PCK-26] (AB6401)")
Publications
Filter by
- Science advances 9:eadg51092023Coinhibition of topoisomerase 1 and BRD4-mediated pause release selectively kills pancreatic cancer via readthrough transcription.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDonald P Cameron,Jan Grosser,Swetlana Ladigan,Vladislav Kuzin,Evanthia Iliopoulou,Anika Wiegard,Hajar Benredjem,Kathryn Jackson,Sven T Liffers,Smiths Lueong,Phyllis F Cheung,Deepak Vangala,Michael Pohl,Richard Viebahn,Christian Teschendorf,Heiner Wolters,Selami Usta,Keyi Geng,Claudia Kutter,Marie Arsenian-Henriksson,Jens T Siveke,Andrea Tannapfel,Wolff Schmiegel,Stephan A Hahn,Laura BaranelloPubMed 37831776
- Oncogenesis 12:232023Retinoic acid receptor β modulates mechanosensing and invasion in pancreatic cancer cells via myosin light chain 2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCarlos Matellan,Dariusz Lachowski,Ernesto Cortes,Kai Ning Chiam,Aleksandar Krstic,Stephen D Thorpe,Armando E Del Río HernándezPubMed 37130839
- Cancer & metabolism 10:242022Functional noninvasive detection of glycolytic pancreatic ductal adenocarcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIrina Heid,Corinna Münch,Sinan Karakaya,Smiths S Lueong,Alina M Winkelkotte,Sven T Liffers,Laura Godfrey,Phyllis F Y Cheung,Konstantinos Savvatakis,Geoffrey J Topping,Florian Englert,Lukas Kritzner,Martin Grashei,Andrea Tannapfel,Richard Viebahn,Heiner Wolters,Waldemar Uhl,Deepak Vangala,Esther M M Smeets,Erik H J G Aarntzen,Daniel Rauh,Wilko Weichert,Jörg D Hoheisel,Stephan A Hahn,Franz Schilling,Rickmer Braren,Marija Trajkovic-Arsic,Jens T SivekePubMed 36494842
- Cellular & molecular immunology 19:777-7902022IFN-γ cytotoxic CD4 T lymphocytes are involved in the pathogenesis of colitis induced by IL-23 and the food colorant Red 40.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLili Chen,Zhengxiang He,Bernardo S Reis,Jesse D Gelles,Jerry Edward Chipuk,Adrian T Ting,Julie A Spicer,Joseph A Trapani,Glaucia C Furtado,Sergio A LiraPubMed 35468944
- Nature communications 12:57612021Intrathymic differentiation of natural antibody-producing plasma cells in human neonates.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHector Cordero,Rodney G King,Pranay Dogra,Chloe Dufeu,Sarah B See,Alexander M Chong,Anne-Catrin Uhlemann,Siu-Hong Ho,David M Kalfa,Emile A Bacha,John F Kearney,Emmanuel ZornPubMed 34599177
- Oncotarget 12:534-5482021Genomic and neoantigen evolution from primary tumor to first metastases in head and neck squamous cell carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCharles R Schutt,Hua Sun,Jaya Sarin Pradhan,Yvonne Saenger,Jessica Ley,Douglas Adkins,Matthew Ingham,Li Ding,Brian A Van TinePubMed 33796222
- Oncoimmunology 10:18795302021Stromal-like Wilms tumor cells induce human Natural Killer cell degranulation and display immunomodulatory properties towards NK cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesClaudia Cantoni,Martina Serra,Erica Parisi,Bruno Azzarone,Angela Rita Sementa,Luigi Aurelio Nasto,Lorenzo Moretta,Giovanni Candiano,Cristina Bottino,Gian Marco Ghiggeri,Grazia Maria SpaggiariPubMed 33758675
- The Laryngoscope 129:E355-E3642019Methodology for the establishment of primary porcine vocal fold epithelial cell cultures.Applications:Unspecified applicationReactive species:Unspecified reactive speciesElizabeth Erickson-DiRenzo et. al.PubMed 30848488
- Journal of cellular biochemistry 120:9213-92292018Peroxisome proliferator-activated receptor-α agonist and all-trans retinoic acid induce epithelial differentiation of subcutaneous adipose-derived stem cells from debrided burn skin.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShanmugasundaram Natesan,Nicole L Wrice,Robert J ChristyPubMed 30556162
- Veterinary pathology 56:157-1682018Proliferative Typhlocolitis With Multinucleated Giant Cells: A Nonspecific Enteropathy in Immunodeficient Sentinel Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKerriann M Casey,Amanda L Johnson,Melea N Hunrath,Jenelle K Fraser,Nicole C McCowan,Katherine Wasson,Rosalinda A Doty,Stephen M Griffey,Denise M ImaiPubMed 30222063
Key facts
- Isotype
IgG1
- Host species
Mouse
- Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- Native Full Length Protein corresponding to Human KRT76. Database link Q01546
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Rat | Predicted |
| Chicken | Predicted |
| Cow | Predicted |
| Goat | Predicted |
| Rabbit | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Rabbit, Goat, Chicken, Cow | Dilution info - | Notes - |
Associated Products
Select an associated product type
15 products for Alternative Product
Alternative names
Cytokeratin-2P, Keratin-76, Type-II keratin Kb9, CK-2P, K2P, K76, KRT2P, KRT2B, KRT76
Recommended products
Mouse Monoclonal Cytokeratin 2P antibody. Suitable for WB and reacts with Human samples. Cited in 45 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human KRT76.
Key facts
- Isotype
IgG1
- Host species
Mouse
- Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- Native Full Length Protein corresponding to Human KRT76. Database link Q01546
- Clone number
PCK-26
- Purity
Tissue culture supernatant
- Light chain type
unknown
- Concentration
- Purification notes
Purified from TCS.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
This product was changed from ascites to tissue culture supernatant on 17 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Pan cytokeratin also known as cytokeratin AE1/AE3 is a cytoskeletal protein complex that consists of different keratin polypeptides. It mechanically supports epithelial cells contributing to their structural integrity. The protein mass is variable due to its composite nature ranging between 40 to 67 kDa. Pan cytokeratin is expressed in epithelial tissues throughout the body serving as a reliable marker for identifying epithelial origin cells in histological studies.
- Biological function summary
Keratins in pan cytokeratin contribute to maintaining structural stability within epithelial cells. They act as components of the cytoskeletal network interacting with other cellular structures to provide protection from stress and mechanical damage. Pan cytokeratin exists as part of the intermediate filament protein complex which plays a significant role in cellular mechanics and cohesion essential for tissue integrity.
- Pathways
Intermediate filaments containing pan cytokeratin participate in pathways related to cell differentiation and stress response. Its interactions with other proteins like desmoplakin and plectin link the cytoskeletal filaments to cell membranes and the extracellular matrix. This involvement is important in pathways related to cellular structural integrity and epithelial-mesenchymal transition (EMT).
- Associated diseases and disorders
Pan cytokeratin serves as a critical marker in the diagnosis of epithelial-derived cancers such as carcinoma and conditions like psoriasis. Abnormal expression patterns of pan cytokeratin indicate tumor presence and origin. Its connection to proteins such as E-cadherin and vimentin assists in understanding the progression and metastasis of cancer by facilitating EMT and tissue invasion diagnostics.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-pan Cytokeratin antibody [PCK-26] (ab6401), expandable thumbnail](https://content.abcam.com/products/images/pan-cytokeratin-antibody-pck-26-ab6401--western-blot-img12340.jpg "Western blot - Anti-pan Cytokeratin antibody [PCK-26] (ab6401)")
Western blot - Anti-pan Cytokeratin antibody [PCK-26] (ab6401)
This image was generated using the ascites version of the product.
Lane 1: Western blot - Anti-pan Cytokeratin antibody [PCK-26] (ab6401) at 2.5 µg/mL
Lane 2: Western blot - Anti-pan Cytokeratin antibody [PCK-26] (ab6401) at 5 µg/mL
Lane 3: Western blot - Anti-pan Cytokeratin antibody [PCK-26] (ab6401) at 10 µg/mL
All lanes: HeLa cell lysate
SecondaryAll lanes: Peroxidase-conjugated goat anti-mouse IgG
![Western blot - Anti-pan Cytokeratin antibody [PCK-26] (ab6401), expandable thumbnail](https://content.abcam.com/products/images/pan-cytokeratin-antibody-pck-26-ab6401--western-blot-img97282.jpg "Western blot - Anti-pan Cytokeratin antibody [PCK-26] (ab6401)")
This image is courtesy of a customer review submitted by Pawel MazurWestern blot - Anti-pan Cytokeratin antibody [PCK-26] (ab6401)
All lanes: pan Cytokeratin antibody (ab6401) at 1/1000 dilution + whole cell lysate of Primary mouse pancreatic cancer cell lines (35 μg)
Secondary: An HRP-conjugated Sheep anti-mouse IgG polyclonal (1/5000) developed using the ECL technique
Performed under non-reducing conditions.
Blocking Step: 10% Milk for 1 hour at room temperatureThis image was generated using the ascites version of the product.
All lanes: Western blot - Anti-pan Cytokeratin antibody [PCK-26] (ab6401)
Observed band size: 55 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com