AB199481

Anti-Pan-Farnesylation antibody [EPR19409]

RabMAb
Recombinant
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(3 Publications)

Rabbit Recombinant Monoclonal Pan-Farnesylation antibody. Suitable for Dot, WB and reacts with Synthetic peptide, Human samples. Cited in 3 publications.

2 Images

Supplier Data

Western blot - Anti-Pan-Farnesylation antibody [EPR19409] (AB199481)

Blocking/Dilution buffer : 5% NFDM/TBST.

The lysates were kindly provided by the collaborator (Dr. Sheng-Cai Lin, Xiamen University). Based on the sequence alignment, this product may recognize human, mouse and rat species. The ab was developed specifically for farnesylated LKB1. Refer to the literature (PMID : 24295069), it might also interact with other farnesylated proteins.

All lanes:

Western blot - Anti-Pan-Farnesylation antibody [EPR19409] (ab199481) at 1/1000 dilution

Lane 1:

HA-LKB1(WT) transfected 293T (Human epithelial cell line from embryonic kidney) lysate at 10 µg

Lane 2:

HA-LKB1(C433S) transfected 293T (Human epithelial cell line from embryonic kidney) lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 46 kDa,49 kDa,8 kDa,82 kDa,86 kDa

Observed band size: 110 kDa,46 kDa

false

Exposure time: 2s

Supplier Data

Dot Blot - Anti-Pan-Farnesylation antibody [EPR19409] (AB199481)

Dot blot analysis of Pan-Farnesylation labeled with ab199481 at 1/1000 dilution.

Lane 1 : LKB1 farnesylated peptide;

Lane 2 : LKB1 non-farnesylated peptide.

Goat Anti-Rabbit IgG (H+L) Peroxidase conjugated (ab97051) at 1/100000 dilution was used as secondary antibody.

Blocking and diluting buffer : 5% NFDM/TBST.

Exposure time : 3 minutes.

Carrier free

Anti-Pan-Farnesylation antibody [EPR19409] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19409

Isotype

IgG

Carrier free

Reacts with

Human

Applications

Dot, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The antibody was developed specifically for farnesylated LKB1. Refer to the literature (PMID: 24295069), it might also interact with other farnesylated proteins.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Synthetic peptide": { "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Pan-farnesylation refers to a post-translational modification where a farnesyl group attaches to proteins allowing them to anchor to cellular membranes. This process involves farnesylated proteins which include small GTPases like Ras Rho and nuclear lamins. The farnesyl group derived from farnesyl diphosphate is a 15-carbon isoprenoid lipid precursor. Farnesylation occurs in eukaryotic cells and is mediated by farnesyltransferase which recognizes the CaaX motif on the target protein. This modification happens mostly in the cytoplasm and enables proteins to associate with the inner nuclear membrane and the membrane of the endoplasmic reticulum.

Biological function summary

This lipid modification facilitates protein-protein interactions and membrane localization required for signal transduction and cell cycle regulation. Farnesylation is essential for the function of many proteins but does not happen as part of a complex. It plays a role in the structural integrity and function of the cell by helping to establish protein orientation and interaction networks at the membrane surface. Farnesylated proteins like Ras participate in transmitting signals from extracellular receptors to intracellular targets which influence cell proliferation and differentiation.

Pathways

Farnesylation is a critical step in the post-translational modification pathways enabling proper localization and activation of signaling molecules. It is heavily implicated in the Ras-MAPK signaling pathway where farnesylated Ras proteins take part in cellular responses to growth factors. Different members of the Ras family undergo this modification influencing downstream signaling pathways like ERK. Additionally the farnesylation of nuclear lamins integrates into pathways concerning nuclear envelope organization and integrity essential for maintaining cellular architecture.

Impaired farnesylation processes can lead to pathologies such as cancer and progeria. Farnesylation of Ras proteins links to oncogenesis as mutations in ras genes result in constitutively active farnesylated Ras promoting uncontrolled cell division in many cancers. In Hutchinson-Gilford progeria syndrome lack of proper lamin A farnesylation affects nuclear structure leading to premature aging. These connections highlight the significance of farnesylation in maintaining normal cellular function and how its dysregulation can contribute to severe diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Pan-Farnesylation

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Acta physiologica (Oxford, England) 241:e14279 PubMed39803764

2025

Left superior cervical ganglia lymph node mimicry and its role in rat ventricular arrhythmias following myocardial infarction.

Applications

Unspecified application

Species

Unspecified reactive species

Qingxia Yu,Yan Li,Wenju Yan,Weizhong Han,Qian Liu,Junyi Zhang,Xiaolu Li,Yugen Shi,Yu Wang,Jie Yin,Suhua Yan

The EMBO journal 44:1185-1219 PubMed39762645

2025

RHEB neddylation by the UBE2F-SAG axis enhances mTORC1 activity and aggravates liver tumorigenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Fengwu Zhang,Xiufang Xiong,Zhijian Li,Haibo Wang,Weilin Wang,Yongchao Zhao,Yi Sun

Experimental and therapeutic medicine 19:2861-2870 PubMed32256770

2020

Effects of mevalonate kinase interference on cell differentiation, apoptosis, prenylation and geranylgeranylation of human keratinocytes are attenuated by farnesyl pyrophosphate or geranylgeranyl pyrophosphate.

Applications

Unspecified application

Species

Unspecified reactive species

Min Li,Wei Min,Jianbo Wang,Lu Wang,Yan Li,Naihui Zhou,Ziliang Yang,Qihong Qian

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Pan-Farnesylation antibody [EPR19409]

Western blot - Anti-Pan-Farnesylation antibody [EPR19409] (AB199481)

Dot Blot - Anti-Pan-Farnesylation antibody [EPR19409] (AB199481)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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