AB251276

Anti-Pan-Farnesylation antibody [EPR19409] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal Pan-Farnesylation antibody. Carrier free. Suitable for WB, Dot and reacts with Transfected cell lysate, Human, Synthetic peptide samples.

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Supplier Data

Western blot - Anti-Pan-Farnesylation antibody [EPR19409] - BSA and Azide free (AB251276)

This data was developed using ab199481, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

The lysates were kindly provided by the collaborator (Dr. Sheng-Cai Lin, Xiamen University). Based on the sequence alignment, this product may recognize human, mouse and rat species. The ab was developed specifically for farnesylated LKB1. Refer to the literature (PMID : 24295069), it might also interact with other farnesylated proteins.

All lanes:

Western blot - Anti-Pan-Farnesylation antibody [EPR19409] (<a href='/en-us/products/primary-antibodies/pan-farnesylation-antibody-epr19409-ab199481'>ab199481</a>) at 1/1000 dilution

Lane 1:

HA-LKB1(WT) transfected 293T (Human epithelial cell line from embryonic kidney) lysate at 10 µg

Lane 2:

HA-LKB1(C433S) transfected 293T (Human epithelial cell line from embryonic kidney) lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 46 kDa,49 kDa,8 kDa,82 kDa,86 kDa

Observed band size: 110 kDa,46 kDa

false

Exposure time: 2s

Supplier Data

Dot Blot - Anti-Pan-Farnesylation antibody [EPR19409] - BSA and Azide free (AB251276)

This data was developed using ab199481, the same antibody clone in a different buffer formulation.

Dot blot analysis of Pan-Farnesylation labeled with ab199481 at 1/1000 dilution.

Lane 1 : LKB1 farnesylated peptide;
Lane 2 : LKB1 non-farnesylated peptide.

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution was used as secondary antibody.

Blocking and diluting buffer : 5% NFDM/TBST.

Exposure time : 3 minutes.

Unconjugated

Anti-Pan-Farnesylation antibody [EPR19409]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19409

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

Dot, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The antibody was developed specifically for farnesylated LKB1. Refer to the literature (PMID: 24295069), it might also interact with other farnesylated proteins.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "" }, "Synthetic peptide": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "" }, "Transfected cell lysate": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "Dot-species-checked": "notRecommended", "Dot-species-dilution-info": "", "Dot-species-notes": "" } } }

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Product details

ab251276 is the carrier-free version of ab199481.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Pan-farnesylation refers to a post-translational modification where a farnesyl group attaches to proteins allowing them to anchor to cellular membranes. This process involves farnesylated proteins which include small GTPases like Ras Rho and nuclear lamins. The farnesyl group derived from farnesyl diphosphate is a 15-carbon isoprenoid lipid precursor. Farnesylation occurs in eukaryotic cells and is mediated by farnesyltransferase which recognizes the CaaX motif on the target protein. This modification happens mostly in the cytoplasm and enables proteins to associate with the inner nuclear membrane and the membrane of the endoplasmic reticulum.

Biological function summary

This lipid modification facilitates protein-protein interactions and membrane localization required for signal transduction and cell cycle regulation. Farnesylation is essential for the function of many proteins but does not happen as part of a complex. It plays a role in the structural integrity and function of the cell by helping to establish protein orientation and interaction networks at the membrane surface. Farnesylated proteins like Ras participate in transmitting signals from extracellular receptors to intracellular targets which influence cell proliferation and differentiation.

Pathways

Farnesylation is a critical step in the post-translational modification pathways enabling proper localization and activation of signaling molecules. It is heavily implicated in the Ras-MAPK signaling pathway where farnesylated Ras proteins take part in cellular responses to growth factors. Different members of the Ras family undergo this modification influencing downstream signaling pathways like ERK. Additionally the farnesylation of nuclear lamins integrates into pathways concerning nuclear envelope organization and integrity essential for maintaining cellular architecture.

Impaired farnesylation processes can lead to pathologies such as cancer and progeria. Farnesylation of Ras proteins links to oncogenesis as mutations in ras genes result in constitutively active farnesylated Ras promoting uncontrolled cell division in many cancers. In Hutchinson-Gilford progeria syndrome lack of proper lamin A farnesylation affects nuclear structure leading to premature aging. These connections highlight the significance of farnesylation in maintaining normal cellular function and how its dysregulation can contribute to severe diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Pan-Farnesylation

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Pan-Farnesylation antibody [EPR19409] - BSA and Azide free

Western blot - Anti-Pan-Farnesylation antibody [EPR19409] - BSA and Azide free (AB251276)

Dot Blot - Anti-Pan-Farnesylation antibody [EPR19409] - BSA and Azide free (AB251276)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise