AB8068

Anti-pan Keratin antibody [80]

Name: Anti-pan Keratin antibody [80] (ab8068) | Abcam
Brand: Abcam Limited
SKU: AB8068
Price: 545 USD
Availability: InStock
Rating: 5 (12 reviews)

(12 Reviews)

(47 Publications)

Anti-pan Keratin antibody [80] (ab8068) is a mouse monoclonal antibody detecting pan Keratin in Western Blot, Flow Cytometry, IHC-P, IHC-Fr, ICC/IF. Suitable for Human, Mouse, Rat.

- Over 40 publications
- Trusted since 2001

View Alternative Names

KRTA, KRT1, 67 kDa cytokeratin, Cytokeratin-1, Hair alpha protein, Keratin-1, Type-II keratin Kb1, CK-1, K1

4 Images

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Western blot - Anti-pan Keratin antibody [80] (AB8068)

All lanes:

Western blot - Anti-pan Keratin antibody [80] (ab8068) at 1/250 dilution

All lanes:

A431 (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-mouse-igg-h-l-hrp-preadsorbed-ab97040'>ab97040</a>) at 1/5000 dilution

Predicted band size: 65 kDa

Observed band size: 49 kDa,58 kDa,65 kDa

true

Exposure time: 30s

Immunocytochemistry/ Immunofluorescence - Anti-pan Keratin antibody [80] (AB8068)

ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-pan Keratin antibody [80] (AB8068)

ICC/IF image of ab8068 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab8068, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

ICC/IF

AbReview27846****

Immunocytochemistry/ Immunofluorescence - Anti-pan Keratin antibody [80] (AB8068)

ab8068 staining pan Keratin in Human glioblastoma cell line by ICC/IF (Immunocytochemistry/Immunofluorescence). The cells were fixed with paraformaldehyde; permeabilized with 0.1% Triton X 100 in PBS and blocked with 0.5% BSA for 30 minutes at room temperature. Samples were incubated with primary antibody (1/50 dilution in 0.5% BSA in PBS) for 16hours at 4°C. An Alexa Fluor^® 488 anti-mouse was used as a secondary antibody at 1/400 dilution.

This image is courtesy of an anonymous Abreview

Flow Cytometry - Anti-pan Keratin antibody [80] (AB8068)

Flow Cyt

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Flow Cytometry - Anti-pan Keratin antibody [80] (AB8068)

Overlay histogram showing A431 cells stained with ab8068 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab8068, 1/20 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1](ab91353, 2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

Isotype

IgG1

Carrier free

Reacts with

Human, Mouse, Rat

Applications

IHC-Fr, WB, ICC/IF, Flow Cyt, IHC-P

applications

Immunogen

Native Full Length Protein corresponding to Human KRT1.

P04264

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/20", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "For paraffin embedded tissue a TUF pretreatment is recommended.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "Stains all types of keratin containing cells (epithelia) in frozen sections of various tissues, with the exception of myoepithelial cells." }, "Mouse": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "For paraffin embedded tissue a TUF pretreatment is recommended.", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "Stains all types of keratin containing cells (epithelia) in frozen sections of various tissues, with the exception of myoepithelial cells." }, "Rat": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "For paraffin embedded tissue a TUF pretreatment is recommended.", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "Stains all types of keratin containing cells (epithelia) in frozen sections of various tissues, with the exception of myoepithelial cells." } } }

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Product details

What is this antibody validated in?
Anti-pan Keratin antibody [80] (ab8068) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.

What is the molecular weight of pan Keratin?
Anti-pan Keratin [80] (ab8068) specifically detects a band for pan Keratin (UniProt: P35908) at a molecular weight of 65kDa.

Trusted by the scientific community
Anti-pan Keratin [80] (ab8068) was first used in a scientific publication in 2001 and has been cited over 40 times in peer-reviewed journals.

Reviewed by scientists
Anti-pan Keratin [80] (ab8068) has over 10 independent reviews from customers.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.3 Preservative: 0.07% Sodium azide Constituents: 1% Fetal calf serum

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Pan Keratin also known as pan-keratin is a complex mixture of intermediate filament proteins essential for the mechanical stability of epithelial cells. These keratins have a range of molecular weights between 40 and 67 kDa. Keratin expression occurs in epithelial tissues where they form a dense network providing structural support. Researchers commonly employ anti-keratin antibodies and anti-pan keratin antibodies to study various epithelial tissues as these antibodies broadly recognize keratin proteins allowing for a comprehensive pan keratin stain in histological applications.

Biological function summary

Keratins contribute to cellular integrity and resilience against mechanical stress. They function within the cytoskeleton by forming filamentous networks that link to desmosomes. These filaments involve several keratin types and non-keratin proteins as part of a larger complex. Pan keratin immunostain techniques help visualize these structures demonstrating their important role in maintaining epithelial barrier function and tissue architecture which includes stratified and simple epithelia. The interaction of keratins with other cytoskeletal elements underlines their significance in cellular biology.

Pathways

Keratins are integral to the cytoskeleton organization and cell adhesion pathways. Cell junctions such as desmosomes rely on keratin filaments for stability and signal transduction. Connections exist with proteins such as desmoplakin and plakoglobin which are important in maintaining cell-cell adhesion and communication. Researchers often use keratin stains to examine epithelial tissue organization giving insights into disruptions within these pathways that can affect tissue function.

Keratin expression patterns serve as markers in diagnosing epithelial-derived tumors such as carcinomas and certain skin disorders. Abnormal keratin expression can indicate epithelial tissue transformation contributing to cancer development or progression. For example variations in specific keratin types are markers for different carcinoma types. Moreover disorders involving the skin like epidermolysis bullosa simplex may relate to mutations in keratin genes. Understanding these connections enables researchers to explore potential therapeutic approaches by targeting related proteins and pathways.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May regulate the activity of kinases such as PKC and SRC via binding to integrin beta-1 (ITB1) and the receptor of activated protein C kinase 1 (RACK1). In complex with C1QBP is a high affinity receptor for kininogen-1/HMWK.

See full target information KRT1

Additional targets

KRT16,KRT19,KRT6C,KRT15,KRT18,Keratin, type II cytoskeletal 5,KRT6B,Keratin, type I cytoskeletal 14,KRT4,KRT12,Keratin, type I cytoskeletal 13,Keratin, type I cytoskeletal 9,KRT20,KRT3,KRT2,KRT8,KRT17,KRT6A,KRT7,KRT10

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Publications (47)

Recent publications for all applications. Explore the full list and refine your search

Experimental and therapeutic medicine 29:33 PubMed40486898

2025

Effect of overexpression of on the proliferation and osteogenic capacity of human periodontal cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xuanxuan Yao,Ruoshan Qin,Ziwei Cui,Dengqi He,Xiaorong Sun,Yuefeng Sun,Xiangyi He

Oncology research 33:1459-1472 PubMed40486881

2025

Apatinib modulates sorafenib-resistant hepatocellular carcinoma through inhibiting the EGFR/JNK/ERK signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Dexue Fan,Wei Su,Zhaowen Bi,Xinxing Wang,Xianwen Xu,Mingze Ma,Lichao Zhu,Zhenhai Zhang,Junlin Gao

Frontiers in veterinary science 11:1465879 PubMed39444741

2024

Periodontal ligament stem cell tissue engineering scaffolds can guide and promote canine periodontal tissue regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Pengxiu Dai,Guixiang Qi,Mingde Zhu,Qingjie Du,Keyi Wang,Yaxin Gao,Mengnan Li,Xiancheng Feng,Xinke Zhang

Open life sciences 18:20220711 PubMed37671092

2023

TRIM29 knockdown prevented the colon cancer progression through decreasing the ubiquitination levels of KRT5.

Applications

Unspecified application

Species

Unspecified reactive species

Lihui Sun,Dawei Wang,Zhenyu Chen,Xu Zhu

Clinical, cosmetic and investigational dermatology 16:1457-1472 PubMed37309428

2023

Study on the Key Genes and Molecular Mechanisms of IL-27 Promoting Keratinocytes Proliferation Based on Transcriptome Sequencing.

Applications

Unspecified application

Species

Unspecified reactive species

Zijun Wu,Qin Yang,Kai Xu,Juanjuan Wu,Bin Yang

Viruses 15: PubMed37243252

2023

Apical-Out Human Airway Organoids Modeling SARS-CoV-2 Infection.

Applications

Unspecified application

Species

Unspecified reactive species

Man Chun Chiu,Shuxin Zhang,Cun Li,Xiaojuan Liu,Yifei Yu,Jingjing Huang,Zhixin Wan,Xiaoxin Zhu,Jie Zhou

International journal of molecular sciences 24: PubMed37047109

2023

Effect of Hypertrophic Scar Fibroblast-Derived Exosomes on Keratinocytes of Normal Human Skin.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Song Cui,So Young Joo,Seung Yeol Lee,Yoon Soo Cho,Dong Hyun Kim,Cheong Hoon Seo

Histopathology 81:847-849 PubMed36063135

2022

Myoepithelioma-like hyalinising epithelioid tumour of the foot: biopsy diagnosis, with molecular confirmation.

Applications

Unspecified application

Species

Unspecified reactive species

Azfar Neyaz,Reeba A Omman,Abigail I Wald,Armando Herradura,Karen J Fritchie,Ivy John

Molecular and cellular biochemistry 477:1959-1971 PubMed35380292

2022

PPARγ alleviates peritoneal fibrosis progression along with promoting GLUT1 expression and suppressing peritoneal mesothelial cell proliferation.

Applications

Unspecified application

Species

Unspecified reactive species

Junxia Feng,Meizhi Lu,Wenhao Li,Jingchun Li,Ping Meng,Zukai Li,Xuejuan Gao,Yunfang Zhang

Experimental and therapeutic medicine 23:343 PubMed35401799

2022

LINC01605 knockdown induces apoptosis in human Tenon's capsule fibroblasts by inhibiting autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Qifei Shang,Yanhua Yang,Hangzhu Li

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-pan Keratin antibody [80]

Western blot - Anti-pan Keratin antibody [80] (AB8068)

Immunocytochemistry/ Immunofluorescence - Anti-pan Keratin antibody [80] (AB8068)

Immunocytochemistry/ Immunofluorescence - Anti-pan Keratin antibody [80] (AB8068)

Flow Cytometry - Anti-pan Keratin antibody [80] (AB8068)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (47)

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