AB190625

Anti-pan Keratin antibody

Name: Anti-pan Keratin antibody (ab190625) | Abcam
Brand: Abcam Limited
SKU: AB190625
Price: 535 USD
Availability: InStock

(1 Review)

(4 Publications)

Rabbit Polyclonal K22E antibody. Suitable for WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human KRT2 aa 150-200.

View Alternative Names

KRT2A, KRT2E, KRT2, Cytokeratin-2e, Epithelial keratin-2e, Keratin-2 epidermis, Keratin-2e, Type-II keratin Kb2, CK-2e, K2e

1 Images

Supplier Data

Western blot - Anti-pan Keratin antibody (AB190625)

All lanes:

Western blot - Anti-pan Keratin antibody (ab190625) at 1/500 dilution

All lanes:

extracts from Jurkat cells at 30 µg

Predicted band size: 65 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Synthetic Peptide within Human KRT2 aa 150-200. The exact immunogen used to generate this antibody is proprietary information.

P35908

Specificity

This antibody detects endogenous levels of Keratin-pan 185 AND OTHERS protein.

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Reactivity data

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Pan Keratin also known as pan-keratin is a complex mixture of intermediate filament proteins essential for the mechanical stability of epithelial cells. These keratins have a range of molecular weights between 40 and 67 kDa. Keratin expression occurs in epithelial tissues where they form a dense network providing structural support. Researchers commonly employ anti-keratin antibodies and anti-pan keratin antibodies to study various epithelial tissues as these antibodies broadly recognize keratin proteins allowing for a comprehensive pan keratin stain in histological applications.

Biological function summary

Keratins contribute to cellular integrity and resilience against mechanical stress. They function within the cytoskeleton by forming filamentous networks that link to desmosomes. These filaments involve several keratin types and non-keratin proteins as part of a larger complex. Pan keratin immunostain techniques help visualize these structures demonstrating their important role in maintaining epithelial barrier function and tissue architecture which includes stratified and simple epithelia. The interaction of keratins with other cytoskeletal elements underlines their significance in cellular biology.

Pathways

Keratins are integral to the cytoskeleton organization and cell adhesion pathways. Cell junctions such as desmosomes rely on keratin filaments for stability and signal transduction. Connections exist with proteins such as desmoplakin and plakoglobin which are important in maintaining cell-cell adhesion and communication. Researchers often use keratin stains to examine epithelial tissue organization giving insights into disruptions within these pathways that can affect tissue function.

Keratin expression patterns serve as markers in diagnosing epithelial-derived tumors such as carcinomas and certain skin disorders. Abnormal keratin expression can indicate epithelial tissue transformation contributing to cancer development or progression. For example variations in specific keratin types are markers for different carcinoma types. Moreover disorders involving the skin like epidermolysis bullosa simplex may relate to mutations in keratin genes. Understanding these connections enables researchers to explore potential therapeutic approaches by targeting related proteins and pathways.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Probably contributes to terminal cornification (PubMed : 1380918). Associated with keratinocyte activation, proliferation and keratinization (PubMed : 12598329). Required for maintenance of corneocytes and keratin filaments in suprabasal keratinocytes in the epidermis of the ear, potentially via moderation of expression and localization of keratins and their partner proteins (By similarity). Plays a role in the establishment of the epidermal barrier on plantar skin (By similarity).

See full target information KRT2

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Open life sciences 15:1000-1012 PubMed33817286

2020

Long noncoding RNA FTX ameliorates hydrogen peroxide-induced cardiomyocyte injury by regulating the miR-150/KLF13 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yamin Zhang,Xiaoying Fan,Hua Yang

Biomolecules 10: PubMed32260542

2020

Tick and Host Derived Compounds Detected in the Cement Complex Substance.

Applications

Unspecified application

Species

Unspecified reactive species

Margarita Villar,Iván Pacheco,Octavio Merino,Marinela Contreras,Lourdes Mateos-Hernández,Eduardo Prado,Dina Karen Barros-Picanço,José Francisco Lima-Barbero,Sara Artigas-Jerónimo,Pilar Alberdi,Isabel G Fernández de Mera,Agustín Estrada-Peña,Alejandro Cabezas-Cruz,José de la Fuente

Computational and structural biotechnology journal 18:253-257 PubMed33489003

2020

pathogen infection alters chemical composition of the exoskeleton of hard ticks (Acari: Ixodidae).

Applications

Unspecified application

Species

Unspecified reactive species

José de la Fuente,José Francisco Lima-Barbero,Eduardo Prado,Iván Pacheco,Pilar Alberdi,Margarita Villar

Forensic science international 305:110027 PubMed31704515

2019

Cytoskeletal and extracellular matrix proteins resist the burning of bones.

Applications

Unspecified application

Species

Unspecified reactive species

Rubén Darío Díaz-Martín,Javier R Ambrosio,Ricardo Mondragón Flores,Sirenia Gonzáles-Pozos,Lorena Valencia-Caballero

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-pan Keratin antibody

Western blot - Anti-pan Keratin antibody (AB190625)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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