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AB195207

Anti-pan-myc antibody [EPR18863]

  • 20ul selling size
  • Recombinant
  • Advanced Validation
  • RabMAb
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(4 Publications)

Rabbit Recombinant Monoclonal C-Myc/MYC antibody. Suitable for ChIC/CUT&RUN-seq, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse samples. Cited in 4 publications.

View Alternative Names

BHLHE39, MYC, Myc proto-oncogene protein, Class E basic helix-loop-helix protein 39, Proto-oncogene c-Myc, Transcription factor p64, bHLHe39

10 Images
Immunocytochemistry/ Immunofluorescence - Anti-pan-myc antibody [EPR18863] (AB195207)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-pan-myc antibody [EPR18863] (AB195207)

Immunofluorescent analysis of 4% paraformaldehyde-fixed 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling pan-myc with ab195207 at 1/250 dilution followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Confocal image showing nuclear staining on HeLa cell line.

The nuclear counterstain is DAPI (blue).

Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).

The negative controls are as follows : -

-ve control 1 : ab195207 at 1/250 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.

-ve control 2 : ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

Flow Cytometry (Intracellular) - Anti-pan-myc antibody [EPR18863] (AB195207)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-pan-myc antibody [EPR18863] (AB195207)

Intracellular Flow Cytometry analysis of HeLa cells labelling pan-myc (red) with purified ab195207 at dilution of 1/60. The secondary antibody used was Alexa Fluorr® 488 goat-anti-rabbit IgG (1/2000). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Isotype control antibody was Rabbit monoclonal IgG (black). The blue line shows cells without incubation with primary antibody and secondary antibody.

Immunocytochemistry/ Immunofluorescence - Anti-pan-myc antibody [EPR18863] (AB195207)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-pan-myc antibody [EPR18863] (AB195207)

Immunofluorescent analysis of 4% paraformaldehyde-fixed 0.1% Triton X-100 permeabilized RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) cells labeling pan-myc with ab195207 at 1/250 dilution followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Confocal image showing nuclear staining on RAW 264.7 cell line.

The nuclear counterstain is DAPI (blue).

Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).

The negative controls are as follows : -

-ve control 1 : ab195207 at 1/250 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.

-ve control 2 : ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

Western blot - Anti-pan-myc antibody [EPR18863] (AB195207)
  • WB

Supplier Data

Western blot - Anti-pan-myc antibody [EPR18863] (AB195207)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-pan-myc antibody [EPR18863] (ab195207) at 1/2000 dilution

Lane 1:

HEK-293 (Human epithelial cells from embryonic kidney) whole cell lysate transfected with empty vector (vector control) at 10 µg

Lane 2:

HEK-293 whole cell lysate transfected with L-Myc with GFP-tag at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 40 kDa,49 kDa,50 kDa

Observed band size: 57 kDa,83 kDa

false

Exposure time: 3min

Western blot - Anti-pan-myc antibody [EPR18863] (AB195207)
  • WB

Supplier Data

Western blot - Anti-pan-myc antibody [EPR18863] (AB195207)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-pan-myc antibody [EPR18863] (ab195207) at 1/1000 dilution

Lane 1:

HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 10 µg

Lane 2:

HEK-293 (Human epithelial cells from embryonic kidney) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 40 kDa,49 kDa,50 kDa

Observed band size: 57 kDa

false

Exposure time: 3min

Western blot - Anti-pan-myc antibody [EPR18863] (AB195207)
  • WB

Supplier Data

Western blot - Anti-pan-myc antibody [EPR18863] (AB195207)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-pan-myc antibody [EPR18863] (ab195207) at 1/2000 dilution

All lanes:

K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 40 kDa,49 kDa,50 kDa

Observed band size: 57 kDa

false

Exposure time: 3min

ChIC/CUT&RUN sequencing - Anti-pan-myc antibody [EPR18863] (AB195207)
  • ChIC/CUT&RUN-seq

Supplier Data

ChIC/CUT&RUN sequencing - Anti-pan-myc antibody [EPR18863] (AB195207)

ChIC/CUT&RUN was performed using a pAG-MNAse at a final concentration of 700 ng/mL, 2 x 10^5 HeLa (Human cervix adenocarcinoma epithelial cell line) cells and 5µg of ab195207 [EPR18863]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control ab172730 is also shown. Additional screenshots of mapped reads can be found in the Protocol booklet in the Product Protocol section. The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.

Western blot - Anti-pan-myc antibody [EPR18863] (AB195207)
  • WB

Supplier Data

Western blot - Anti-pan-myc antibody [EPR18863] (AB195207)

Blocking/Dilution buffer : 5% NFDM/TBST.

The observed molecular weight for the mouse species is slightly higher than human Myc, this could be due to different degree of glycosylation.

All lanes:

Western blot - Anti-pan-myc antibody [EPR18863] (ab195207) at 1/1000 dilution

All lanes:

RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 40 kDa,49 kDa,50 kDa

Observed band size: 60 kDa

false

Exposure time: 30s

Western blot - Anti-pan-myc antibody [EPR18863] (AB195207)
  • WB

Supplier Data

Western blot - Anti-pan-myc antibody [EPR18863] (AB195207)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-pan-myc antibody [EPR18863] (ab195207) at 1/5000 dilution

Lane 1:

HEK-293 (Human epithelial cells from embryonic kidney) whole cell lysate transfected with empty vector (vector control) at 10 µg

Lane 2:

HEK-293 whole cell lysate transfected with n-Myc with GFP-tag at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 40 kDa,49 kDa,50 kDa

Observed band size: 83 kDa

false

Exposure time: 3s

Western blot - Anti-pan-myc antibody [EPR18863] (AB195207)
  • WB

Unknown

Western blot - Anti-pan-myc antibody [EPR18863] (AB195207)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-pan-myc antibody [EPR18863] (ab195207) at 1/1000 dilution

Lane 1:

HEK-293 (Human epithelial cells from embryonic kidney) whole cell lysate transfected with empty vector (vector control) at 10 µg

Lane 2:

HEK-293 whole cell lysate transfected with c-Myc at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 40 kDa,49 kDa,50 kDa

Observed band size: 57 kDa

false

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR18863

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

Flow Cyt (Intra), ICC/IF, ChIC/CUT&RUN-seq, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ChICCUTRUNseq" : {"fullname" : "ChIC/CUT&RUN sequencing", "shortname":"ChIC/CUT&RUN-seq"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ChICCUTRUNseq-species-checked": "testedAndGuaranteed", "ChICCUTRUNseq-species-dilution-info": "5 µg", "ChICCUTRUNseq-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/60", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "ChICCUTRUNseq-species-checked": "guaranteed", "ChICCUTRUNseq-species-dilution-info": "", "ChICCUTRUNseq-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Pan-MYC refers to a group of transcription factors in the MYC family primarily comprising c-Myc N-Myc and L-Myc with c-Myc weighing around 49 kDa. MYC proteins are nuclear phosphoproteins that regulate gene expression. MYC proteins express in various tissues including embryonic stem cells and proliferating cells and they are known for their involvement in cell cycle progression apoptosis and cellular transformation.
Biological function summary

MYC proteins perform an important role in the regulation of transcription by binding to DNA at E-box sequences. They function as part of the MYC/MAX/MAD network a transcriptional complex with MAX as the obligate partner. This network influences genes involved in cellular growth metabolism and differentiation. Deregulation of MYC proteins affects many cellular processes and can influence normal physiology.

Pathways

The MYC proteins are central to the regulation of the cell cycle and Wnt signaling pathways. In the PI3K/Akt signaling MYC interacts with other signaling molecules to control cell growth and survival. The E-box binding allows MYC to regulate multiple downstream targets including cyclins and growth factor receptors. MYC's ability to form a complex with MAX also interlinks it with other survival and proliferation pathways.

Aberrant MYC expression correlates with various cancers such as Burkitt's lymphoma and neuroblastoma. Overexpression of MYC often results from chromosomal translocations or gene amplifications in these malignancies. MYC's connection with the BCL2 protein leads to the disruption of apoptosis promoting oncogenic transformation. Control of MYC expression and function remains a critical focus in cancer research due to its role in tumor development.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3' (PubMed : 24940000, PubMed : 25956029). Activates the transcription of growth-related genes (PubMed : 24940000, PubMed : 25956029). Binds to the VEGFA promoter, promoting VEGFA production and subsequent sprouting angiogenesis (PubMed : 24940000, PubMed : 25956029). Regulator of somatic reprogramming, controls self-renewal of embryonic stem cells (By similarity). Functions with TAF6L to activate target gene expression through RNA polymerase II pause release (By similarity). Positively regulates transcription of HNRNPA1, HNRNPA2 and PTBP1 which in turn regulate splicing of pyruvate kinase PKM by binding repressively to sequences flanking PKM exon 9, inhibiting exon 9 inclusion and resulting in exon 10 inclusion and production of the PKM M2 isoform (PubMed : 20010808).
See full target information MYC
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Pharmaceutics 17: PubMed40430849

2025

The Molecular Chaperone TCP1 Affects Carcinogenicity and Is a Potential Therapeutic Target for Acute Myeloid Leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Yong Wu,Guihui Tu,Yuxia Yuan,Jingwen Liu,Qingna Jiang,Yang Liu,Qiurong Wu,Lixian Wu,Yuanzhong Chen

The Journal of clinical investigation 132: PubMed35775490

2022

Reversal of viral and epigenetic HLA class I repression in Merkel cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Patrick C Lee,Susan Klaeger,Phuong M Le,Keegan Korthauer,Jingwei Cheng,Varsha Ananthapadmanabhan,Thomas C Frost,Jonathan D Stevens,Alan Yl Wong,J Bryan Iorgulescu,Anna Y Tarren,Vipheaviny A Chea,Isabel P Carulli,Camilla K Lemvigh,Christina B Pedersen,Ashley K Gartin,Siranush Sarkizova,Kyle T Wright,Letitia W Li,Jason Nomburg,Shuqiang Li,Teddy Huang,Xiaoxi Liu,Lucas Pomerance,Laura M Doherty,Annie M Apffel,Luke J Wallace,Suzanna Rachimi,Kristen D Felt,Jacquelyn O Wolff,Elizabeth Witten,Wandi Zhang,Donna Neuberg,William J Lane,Guanglan Zhang,Lars R Olsen,Manisha Thakuria,Scott J Rodig,Karl R Clauser,Gabriel J Starrett,John G Doench,Sara J Buhrlage,Steven A Carr,James A DeCaprio,Catherine J Wu,Derin B Keskin

Cell reports. Medicine 2:100344 PubMed34337560

2021

A GPC2 antibody-drug conjugate is efficacious against neuroblastoma and small-cell lung cancer via binding a conformational epitope.

Applications

Unspecified application

Species

Unspecified reactive species

Swetha Raman,Samantha N Buongervino,Maria V Lane,Doncho V Zhelev,Zhongyu Zhu,Hong Cui,Benjamin Martinez,Daniel Martinez,Yanping Wang,Kristen Upton,Khushbu Patel,Komal S Rathi,Carmen T Navia,Daniel B Harmon,Yimei Li,Bruce Pawel,Dimiter S Dimitrov,John M Maris,Jean-Philippe Julien,Kristopher R Bosse

Apoptosis : an international journal on programmed cell death 24:499-510 PubMed30850922

2019

Fibroblasts from patients with idiopathic pulmonary fibrosis are resistant to cisplatin-induced cell death via enhanced CK2-dependent XRCC1 activity.

Applications

Unspecified application

Species

Unspecified reactive species

Jintaek Im,Richard Seonghun Nho
View all publications
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chicCutRunSequencingBooklet
en
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Product promise

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