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Rabbit Recombinant Monoclonal NRX1A antibody. Suitable for WB and reacts with Recombinant fragment - Human, Human, Mouse samples.

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Images

Western blot - Anti-pan-Neurexin antibody [EPR27983-60] (AB322045), expandable thumbnail
  • Western blot - Anti-pan-Neurexin antibody [EPR27983-60] (AB322045), expandable thumbnail
  • Western blot - Anti-pan-Neurexin antibody [EPR27983-60] (AB322045), expandable thumbnail
  • Western blot - Anti-pan-Neurexin antibody [EPR27983-60] (AB322045), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-FrICC/IFIHC-P
Human
Tested
Not recommended
Not recommended
Not recommended
Mouse
Tested
Not recommended
Not recommended
Not recommended
Recombinant fragment - Human
Tested
Not recommended
Not recommended
Not recommended

Tested
Tested

Species

Recombinant fragment - Human

Dilution info

1/1000

Notes

-

Species

Human

Dilution info

1/1000

Notes

-

Species

Mouse

Dilution info

1/1000

Notes

-

Not recommended
Not recommended

Species

Mouse, Human, Recombinant fragment - Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Recombinant fragment - Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Recombinant fragment - Human

Dilution info

-

Notes

-

Target data

Function

Cell surface protein involved in cell-cell-interactions, exocytosis of secretory granules and regulation of signal transmission. Function is isoform-specific. Alpha-type isoforms have a long N-terminus with six laminin G-like domains and play an important role in synaptic signal transmission. Alpha-type isoforms play a role in the regulation of calcium channel activity and Ca(2+)-triggered neurotransmitter release at synapses and at neuromuscular junctions. They play an important role in Ca(2+)-triggered exocytosis of secretory granules in pituitary gland. They may affect their functions at synapses and in endocrine cells via their interactions with proteins from the exocytotic machinery. Likewise, alpha-type isoforms play a role in regulating the activity of postsynaptic NMDA receptors, a subtype of glutamate-gated ion channels. Both alpha-type and beta-type isoforms may play a role in the formation or maintenance of synaptic junctions via their interactions (via the extracellular domains) with neuroligin family members, CBLN1 or CBLN2. In vitro, triggers the de novo formation of presynaptic structures. May be involved in specification of excitatory synapses. Alpha-type isoforms were first identified as receptors for alpha-latrotoxin from spider venom.

Alternative names

KIAA0578, KIAA0578, NRXN1, Neurexin-1, Neurexin I-alpha, Neurexin-1-alpha

Rabbit Recombinant Monoclonal NRX1A antibody. Suitable for WB and reacts with Recombinant fragment - Human, Human, Mouse samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR27983-60

Purification technique

Affinity purification Protein A

Specificity

This antibody recognises Neurexin-1, Neurexin-2 and Neurexin-3.

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-pan-Neurexin antibody [EPR27983-60] (ab322045), expandable thumbnail

    Western blot - Anti-pan-Neurexin antibody [EPR27983-60] (ab322045)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This antibody recognises Neurexin-1, Neurexin-2 and Neurexin-3.

    In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.

    All lanes: Western blot - Anti-pan-Neurexin antibody [EPR27983-60] (ab322045) at 1/1000 dilution

    Lane 1: No tagged human Neurexin-1 protein at 10 ng

    Lane 2: His-tagged human Neurexin-2 protein at 10 ng

    Lane 3: His-tagged human Neurexin-3 protein at 10 ng

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 75-150 kDa

    Exposure time: 37s

  • Western blot - Anti-pan-Neurexin antibody [EPR27983-60] (ab322045), expandable thumbnail

    Western blot - Anti-pan-Neurexin antibody [EPR27983-60] (ab322045)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The expression of Neurexin-1 is downregulated in response to 6-OHDA treatment (PMID: 22155091).

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-pan-Neurexin antibody [EPR27983-60] (ab322045) at 1/1000 dilution

    Lane 1: Untreated SH-SY5Y (human neuroblastoma epithelial cell) whole lysate at 20 µg

    Lane 2: SH-SY5Y (human neuroblastoma epithelial cell) treated 100uM 6-OHDA for 24 hours whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 120 kDa, 36 kDa

    Exposure time: 26s

  • Western blot - Anti-pan-Neurexin antibody [EPR27983-60] (ab322045), expandable thumbnail

    Western blot - Anti-pan-Neurexin antibody [EPR27983-60] (ab322045)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Negative control: lung, tonsil

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-pan-Neurexin antibody [EPR27983-60] (ab322045) at 1/1000 dilution

    Lane 1: Human cerebellum tissue lysate at 40 µg

    Lane 2: Human hippocampus tissue lysate at 40 µg

    Lane 3: Human midbrain tissue lysate at 40 µg

    Lane 4: Human lung tissue lysate at 40 µg

    Lane 5: Human tonsil tissue lysate at 40 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Observed band size: 150-180 kDa, 36 kDa

    Exposure time: 37s

  • Western blot - Anti-pan-Neurexin antibody [EPR27983-60] (ab322045), expandable thumbnail

    Western blot - Anti-pan-Neurexin antibody [EPR27983-60] (ab322045)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Negative control: lung, kidney, colon

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-pan-Neurexin antibody [EPR27983-60] (ab322045) at 1/1000 dilution

    Lane 1: Mouse hypothalamus tissue lysate at 40 µg

    Lane 2: Mouse brain tissue lysate at 40 µg

    Lane 3: Mouse cerebellum tissue lysate at 40 µg

    Lane 4: Mouse lung tissue lysate at 40 µg

    Lane 5: Mouse kidney tissue lysate at 40 µg

    Lane 6: Mouse colon tissue lysate at 40 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Exposure time: 180s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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