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AB322045

Anti-pan-Neurexin antibody [EPR27983-60]

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Rabbit Recombinant Monoclonal NRX1A antibody. Suitable for WB and reacts with Recombinant fragment - Human, Human, Mouse samples.

View Alternative Names

KIAA0578, NRXN1, Neurexin-1, Neurexin I-alpha, Neurexin-1-alpha

4 Images
Western blot - Anti-pan-Neurexin antibody [EPR27983-60] (AB322045)
  • WB

Supplier Data

Western blot - Anti-pan-Neurexin antibody [EPR27983-60] (AB322045)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The expression of Neurexin-1 is downregulated in response to 6-OHDA treatment (PMID : 22155091).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-pan-Neurexin antibody [EPR27983-60] (ab322045) at 1/1000 dilution

Lane 1:

Untreated SH-SY5Y (human neuroblastoma epithelial cell) whole lysate at 20 µg

Lane 2:

SH-SY5Y (human neuroblastoma epithelial cell) treated 100uM 6-OHDA for 24 hours whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 120 kDa,36 kDa

false

Exposure time: 26s

Western blot - Anti-pan-Neurexin antibody [EPR27983-60] (AB322045)
  • WB

Supplier Data

Western blot - Anti-pan-Neurexin antibody [EPR27983-60] (AB322045)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : lung, tonsil

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-pan-Neurexin antibody [EPR27983-60] (ab322045) at 1/1000 dilution

Lane 1:

Human cerebellum tissue lysate at 40 µg

Lane 2:

Human hippocampus tissue lysate at 40 µg

Lane 3:

Human midbrain tissue lysate at 40 µg

Lane 4:

Human lung tissue lysate at 40 µg

Lane 5:

Human tonsil tissue lysate at 40 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 150-180 kDa,36 kDa

false

Exposure time: 37s

Western blot - Anti-pan-Neurexin antibody [EPR27983-60] (AB322045)
  • WB

Supplier Data

Western blot - Anti-pan-Neurexin antibody [EPR27983-60] (AB322045)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : lung, kidney, colon

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-pan-Neurexin antibody [EPR27983-60] (ab322045) at 1/1000 dilution

Lane 1:

Mouse hypothalamus tissue lysate at 40 µg

Lane 2:

Mouse brain tissue lysate at 40 µg

Lane 3:

Mouse cerebellum tissue lysate at 40 µg

Lane 4:

Mouse lung tissue lysate at 40 µg

Lane 5:

Mouse kidney tissue lysate at 40 µg

Lane 6:

Mouse colon tissue lysate at 40 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 150-180 kDa,36 kDa

false

Exposure time: 180s

Western blot - Anti-pan-Neurexin antibody [EPR27983-60] (AB322045)
  • WB

Supplier Data

Western blot - Anti-pan-Neurexin antibody [EPR27983-60] (AB322045)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody recognises Neurexin-1, Neurexin-2 and Neurexin-3.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-pan-Neurexin antibody [EPR27983-60] (ab322045) at 1/1000 dilution

Lane 1:

No tagged human Neurexin-1 protein at 10 ng

Lane 2:

His-tagged human Neurexin-2 protein at 10 ng

Lane 3:

His-tagged human Neurexin-3 protein at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 75-150 kDa

false

Exposure time: 37s

  • Carrier free

    Anti- pan-Neurexin antibody [EPR27983-60] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR27983-60

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody recognises Neurexin-1, Neurexin-2 and Neurexin-3.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Recombinant fragment - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cell surface protein involved in cell-cell-interactions, exocytosis of secretory granules and regulation of signal transmission. Function is isoform-specific. Alpha-type isoforms have a long N-terminus with six laminin G-like domains and play an important role in synaptic signal transmission. Alpha-type isoforms play a role in the regulation of calcium channel activity and Ca(2+)-triggered neurotransmitter release at synapses and at neuromuscular junctions. They play an important role in Ca(2+)-triggered exocytosis of secretory granules in pituitary gland. They may affect their functions at synapses and in endocrine cells via their interactions with proteins from the exocytotic machinery. Likewise, alpha-type isoforms play a role in regulating the activity of postsynaptic NMDA receptors, a subtype of glutamate-gated ion channels. Both alpha-type and beta-type isoforms may play a role in the formation or maintenance of synaptic junctions via their interactions (via the extracellular domains) with neuroligin family members, CBLN1 or CBLN2. In vitro, triggers the de novo formation of presynaptic structures. May be involved in specification of excitatory synapses. Alpha-type isoforms were first identified as receptors for alpha-latrotoxin from spider venom.
See full target information NRXN1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com