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Rabbit Recombinant Multiclonal eNOS antibody. Suitable for Dot, IHC-P and reacts with Recombinant full length protein - Human, Human, Transfected cell line - Human samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan NOS antibody [RM1126] (AB316334), expandable thumbnail
  • Dot Blot - Anti-pan NOS antibody [RM1126] (AB316334), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan NOS antibody [RM1126] (AB316334), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan NOS antibody [RM1126] (AB316334), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan NOS antibody [RM1126] (AB316334), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Multiclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
DotIHC-P
Human
Expected
Tested
Mouse
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Recombinant full length protein - Human
Tested
Not recommended
Transfected cell line - Human
Not recommended
Tested

Tested
Tested

Species

Recombinant full length protein - Human

Dilution info

1/1000

Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Transfected cell line - Human, Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/200

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Transfected cell line - Human

Dilution info

1/2000

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat, Recombinant full length protein - Human

Dilution info

-

Notes

-

Target data

Function

Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.Isoform eNOS13CLacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1.

Targets

NOS1, NOS2, Nos2

Alternative names

Constitutive NOS, EC-NOS, Endothelial NOS, NOS type III, cNOS, eNOS, NOSIII, NOS3

Recommended products

Rabbit Recombinant Multiclonal eNOS antibody. Suitable for Dot, IHC-P and reacts with Recombinant full length protein - Human, Human, Transfected cell line - Human samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Multiclonal

Immunogens
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

RM1126

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

This product is a recombinant multiclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Nitric Oxide Synthases (NOS) are enzymes responsible for the production of nitric oxide (NO) from L-arginine. NOS proteins exist in three main isoforms: neuronal NOS (nNOS or NOS1) inducible NOS (iNOS or NOS2) and endothelial NOS (eNOS or NOS3). They range in molecular mass between 130 and 160 kDa. Pan NOS refers to any antibody or inhibitor that targets all three NOS isoforms. These enzymes are expressed in various tissues with nNOS mainly in neural tissue iNOS in immune cells and eNOS in endothelial cells. Each isoform plays a unique role in its respective location.

Biological function summary

Nitric oxide acts as a signaling molecule with diverse functions within the body. It does not bind into large permanent complexes but interacts transiently with various proteins to relay signals. In the nervous system nNOS-generated NO modulates neurotransmission and contributes to synaptic plasticity. In blood vessels eNOS-derived NO plays an important role in vasodilation and blood pressure regulation. iNOS often gets activated during immune responses producing larger amounts of NO to fight pathogens.

Pathways

Nitric oxide produced by NOS enzymes participates in significant signaling cascades. The L-arginine-NO-cGMP pathway is one of the primary routes involving cyclic guanosine monophosphate (cGMP) as a secondary messenger. The NO-cGMP pathway interacts with proteins like soluble guanylyl cyclase which then modulates various physiological responses like vasodilation and neurotransmission. NOS activity also interplays with oxidative stress pathways interacting with reactive oxygen species (ROS) and affecting cellular responses like inflammation and apoptosis.

Associated diseases and disorders

Aberrations in NOS activity relate to cardiovascular disorders and neurodegenerative diseases. For instance dysregulated eNOS activity can contribute to hypertension due to impaired vasodilation. In neurodegenerative conditions such as Alzheimer's disease abnormal nNOS activity may exacerbate neuronal damage through excessive NO production. Furthermore iNOS expression links to inflammatory conditions where excess NO might damage tissues. NOS activity can also intersect with proteins like amyloid-beta in Alzheimer's amplifying neurodegenerative processes.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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9 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan NOS antibody [RM1126] (ab316334), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan NOS antibody [RM1126] (ab316334)

    Immunohistochemical analysis of paraffin-embedded (A) HEK-293T transfected with a human nNOS expression vector containing a Myc-His tag, (B) HEK-293T transfected with a human iNOS expression vector containing a Myc-His tag, and (C) HEK-293T transfected with a human eNOS expression vector containing a Myc-His tag, no staining on (D) HEK-293T transfected with empty vector containing a Myc-His tag tissues labeling pan NOS with ab316334 at 1/2000 (0.252 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Positive staining on (A) HEK-293T transfected with a human nNOS expression vector containing a Myc-His tag, (B) HEK-293T transfected with a human iNOS expression vector containing a Myc-His tag, and (C) HEK-293T transfected with a human eNOS expression vector containing a Myc-His tag, no staining on (D) HEK-293T transfected with empty vector containing a Myc-His tag. The section was incubated with ab316334 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Dot Blot - Anti-pan NOS antibody [RM1126] (ab316334), expandable thumbnail

    Dot Blot - Anti-pan NOS antibody [RM1126] (ab316334)

    Dot blot analysis of pan NOS using ab316334 at 1:1000 (0.503 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1:20,000 dilution.

    Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] (Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] ab205606) as total protein control staining at 1/1000 dilution.

    All lanes: Dot Blot - Anti-pan NOS antibody [RM1126] (ab316334) at 1/1000 dilution

    Lane 1: C-Myc/DDK tagged full length human nNOS recombinant protein

    Lane 2: C-Myc/DDK tagged full length human iNOS recombinant protein

    Lane 3: C-Myc/DDK tagged full length human eNOS recombinant protein

    Secondary

    All lanes: Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Exposure time: 1s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan NOS antibody [RM1126] (ab316334), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan NOS antibody [RM1126] (ab316334)

    Immunohistochemical analysis of paraffin-embedded Human cerebellum tissue labeling pan NOS with ab316334 at 1/200 (2.515 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Positive staining on human cerebellum. The section was incubated with ab316334 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan NOS antibody [RM1126] (ab316334), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan NOS antibody [RM1126] (ab316334)

    Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling pan NOS with ab316334 at 1/200 (2.515 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Positive staining on endothelial cells in human spleen. The section was incubated with ab316334 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan NOS antibody [RM1126] (ab316334), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan NOS antibody [RM1126] (ab316334)

    Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling pan NOS with ab316334 at 1/200 (2.515 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Positive staining on human lung. The section was incubated with ab316334 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan NOS antibody [RM1126] (ab316334), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan NOS antibody [RM1126] (ab316334)

    Immunohistochemical analysis of paraffin-embedded Human cervical squamous carcinoma tissue labeling pan NOS with ab316334 at 1/200 (2.515 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Positive staining on endothelial cells in human cervical squamous carcinoma. The section was incubated with ab316334 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan NOS antibody [RM1126] (ab316334), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan NOS antibody [RM1126] (ab316334)

    Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling pan NOS with ab316334 at 1/200 (2.515 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Positive staining on human placenta. The section was incubated with ab316334 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan NOS antibody [RM1126] (ab316334), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan NOS antibody [RM1126] (ab316334)

    Immunohistochemical analysis of paraffin-embedded Human thalamus tissue labeling pan NOS with ab316334 at 1/200 (2.515 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Positive staining on human thalamus. The section was incubated with ab316334 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan NOS antibody [RM1126] (ab316334), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan NOS antibody [RM1126] (ab316334)

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling pan NOS with ab316334 at 1/200 (2.515 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Positive staining on human liver. The section was incubated with ab316334 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

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Product protocols

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