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Rabbit Recombinant Multiclonal eNOS antibody. Carrier free. Suitable for Dot, IHC-P and reacts with Recombinant full length protein - Human, Human, Transfected cell line - Human samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan NOS antibody [RM1126] - BSA and Azide free (AB316335), expandable thumbnail
  • Dot Blot - Anti-pan NOS antibody [RM1126] - BSA and Azide free (AB316335), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan NOS antibody [RM1126] - BSA and Azide free (AB316335), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan NOS antibody [RM1126] - BSA and Azide free (AB316335), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan NOS antibody [RM1126] - BSA and Azide free (AB316335), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form

Liquid

Clonality

Multiclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
DotIHC-P
Human
Expected
Tested
Mouse
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Recombinant full length protein - Human
Tested
Not recommended
Transfected cell line - Human
Not recommended
Tested

Tested
Tested

Species

Recombinant full length protein - Human

Dilution info

-

Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Transfected cell line - Human, Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human, Transfected cell line - Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat, Recombinant full length protein - Human

Dilution info

-

Notes

-

Target data

Function

Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.Isoform eNOS13CLacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1.

Targets

Nitric oxide synthase, brain, NOS2, Nos2

Alternative names

Constitutive NOS, EC-NOS, Endothelial NOS, NOS type III, cNOS, eNOS, NOSIII, NOS3

Recommended products

Rabbit Recombinant Multiclonal eNOS antibody. Carrier free. Suitable for Dot, IHC-P and reacts with Recombinant full length protein - Human, Human, Transfected cell line - Human samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Multiclonal

Immunogens
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

RM1126

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Notes

ab316335 is the carrier-free version of Anti-pan NOS antibody [RM1126] ab316334.

This product is a recombinant multiclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Nitric Oxide Synthases (NOS) are enzymes responsible for the production of nitric oxide (NO) from L-arginine. NOS proteins exist in three main isoforms: neuronal NOS (nNOS or NOS1) inducible NOS (iNOS or NOS2) and endothelial NOS (eNOS or NOS3). They range in molecular mass between 130 and 160 kDa. Pan NOS refers to any antibody or inhibitor that targets all three NOS isoforms. These enzymes are expressed in various tissues with nNOS mainly in neural tissue iNOS in immune cells and eNOS in endothelial cells. Each isoform plays a unique role in its respective location.

Biological function summary

Nitric oxide acts as a signaling molecule with diverse functions within the body. It does not bind into large permanent complexes but interacts transiently with various proteins to relay signals. In the nervous system nNOS-generated NO modulates neurotransmission and contributes to synaptic plasticity. In blood vessels eNOS-derived NO plays an important role in vasodilation and blood pressure regulation. iNOS often gets activated during immune responses producing larger amounts of NO to fight pathogens.

Pathways

Nitric oxide produced by NOS enzymes participates in significant signaling cascades. The L-arginine-NO-cGMP pathway is one of the primary routes involving cyclic guanosine monophosphate (cGMP) as a secondary messenger. The NO-cGMP pathway interacts with proteins like soluble guanylyl cyclase which then modulates various physiological responses like vasodilation and neurotransmission. NOS activity also interplays with oxidative stress pathways interacting with reactive oxygen species (ROS) and affecting cellular responses like inflammation and apoptosis.

Associated diseases and disorders

Aberrations in NOS activity relate to cardiovascular disorders and neurodegenerative diseases. For instance dysregulated eNOS activity can contribute to hypertension due to impaired vasodilation. In neurodegenerative conditions such as Alzheimer's disease abnormal nNOS activity may exacerbate neuronal damage through excessive NO production. Furthermore iNOS expression links to inflammatory conditions where excess NO might damage tissues. NOS activity can also intersect with proteins like amyloid-beta in Alzheimer's amplifying neurodegenerative processes.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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