Rabbit Recombinant Multiclonal eNOS antibody. Carrier free. Suitable for Dot, IHC-P and reacts with Recombinant full length protein - Human, Human, Transfected cell line - Human samples.
Images
![Dot Blot - Anti-pan NOS antibody [RM1126] - BSA and Azide free (AB316335), expandable thumbnail](https://content.abcam.com/products/images/pan-nos-antibody-rm1126-bsa-and-azide-free-ab316335--dot-blot-img432199.jpg/_jcr_content/renditions/webp-475.webp "Dot Blot - Anti-pan NOS antibody [RM1126] - BSA and Azide free (AB316335)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan NOS antibody [RM1126] - BSA and Azide free (AB316335), expandable thumbnail](https://content.abcam.com/products/images/pan-nos-antibody-rm1126-bsa-and-azide-free-ab316335--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img432266.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan NOS antibody [RM1126] - BSA and Azide free (AB316335)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan NOS antibody [RM1126] - BSA and Azide free (AB316335), expandable thumbnail](https://content.abcam.com/products/images/pan-nos-antibody-rm1126-bsa-and-azide-free-ab316335--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img432260.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan NOS antibody [RM1126] - BSA and Azide free (AB316335)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan NOS antibody [RM1126] - BSA and Azide free (AB316335), expandable thumbnail](https://content.abcam.com/products/images/pan-nos-antibody-rm1126-bsa-and-azide-free-ab316335--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img432263.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan NOS antibody [RM1126] - BSA and Azide free (AB316335)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan NOS antibody [RM1126] - BSA and Azide free (AB316335), expandable thumbnail](https://content.abcam.com/products/images/pan-nos-antibody-rm1126-bsa-and-azide-free-ab316335--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img432262.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan NOS antibody [RM1126] - BSA and Azide free (AB316335)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Multiclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Dot | IHC-P | |
|---|---|---|
| Human | Expected | Tested |
| Mouse | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended |
| Recombinant full length protein - Human | Tested | Not recommended |
| Transfected cell line - Human | Not recommended | Tested |
Dot
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - Human, Mouse, Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Transfected cell line - Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Recombinant full length protein - Human | Dilution info - | Notes - |
Target data
Function
Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway (PubMed:1378832). NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. Isoform eNOS13C. Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1.
Additional Targets
Nitric oxide synthase, brain, NOS2, Nos2
Alternative names
Nitric oxide synthase 3, Constitutive NOS, EC-NOS, NOS type III, cNOS, NOSIII, Endothelial NOS, eNOS, NOS3
Recommended products
Rabbit Recombinant Multiclonal eNOS antibody. Carrier free. Suitable for Dot, IHC-P and reacts with Recombinant full length protein - Human, Human, Transfected cell line - Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Multiclonal
- Immunogens
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- RM1126
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab316335 is the carrier-free version of Anti-pan NOS antibody [RM1126] ab316334.
What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:
- - The sensitivity of polyclonal antibodies by recognising multiple epitopes
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
View our range of recombinant multiclonal antibodies.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Nitric Oxide Synthases (NOS) are enzymes responsible for the production of nitric oxide (NO) from L-arginine. NOS proteins exist in three main isoforms: neuronal NOS (nNOS or NOS1) inducible NOS (iNOS or NOS2) and endothelial NOS (eNOS or NOS3). They range in molecular mass between 130 and 160 kDa. Pan NOS refers to any antibody or inhibitor that targets all three NOS isoforms. These enzymes are expressed in various tissues with nNOS mainly in neural tissue iNOS in immune cells and eNOS in endothelial cells. Each isoform plays a unique role in its respective location.
- Biological function summary
Nitric oxide acts as a signaling molecule with diverse functions within the body. It does not bind into large permanent complexes but interacts transiently with various proteins to relay signals. In the nervous system nNOS-generated NO modulates neurotransmission and contributes to synaptic plasticity. In blood vessels eNOS-derived NO plays an important role in vasodilation and blood pressure regulation. iNOS often gets activated during immune responses producing larger amounts of NO to fight pathogens.
- Pathways
Nitric oxide produced by NOS enzymes participates in significant signaling cascades. The L-arginine-NO-cGMP pathway is one of the primary routes involving cyclic guanosine monophosphate (cGMP) as a secondary messenger. The NO-cGMP pathway interacts with proteins like soluble guanylyl cyclase which then modulates various physiological responses like vasodilation and neurotransmission. NOS activity also interplays with oxidative stress pathways interacting with reactive oxygen species (ROS) and affecting cellular responses like inflammation and apoptosis.
- Associated diseases and disorders
Aberrations in NOS activity relate to cardiovascular disorders and neurodegenerative diseases. For instance dysregulated eNOS activity can contribute to hypertension due to impaired vasodilation. In neurodegenerative conditions such as Alzheimer's disease abnormal nNOS activity may exacerbate neuronal damage through excessive NO production. Furthermore iNOS expression links to inflammatory conditions where excess NO might damage tissues. NOS activity can also intersect with proteins like amyloid-beta in Alzheimer's amplifying neurodegenerative processes.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
9 product images
![Dot Blot - Anti-pan NOS antibody [RM1126] - BSA and Azide free (ab316335), expandable thumbnail](https://content.abcam.com/products/images/pan-nos-antibody-rm1126-bsa-and-azide-free-ab316335--dot-blot-img432199.jpg "Dot Blot - Anti-pan NOS antibody [RM1126] - BSA and Azide free (ab316335)")
Dot Blot - Anti-pan NOS antibody [RM1126] - BSA and Azide free (ab316335)
Pan NOS Dot Blot staining using rabbit Anti-pan NOS antibody
This data was developed using Anti-pan NOS antibody [RM1126] ab316334, the same antibody clone in a different buffer formulation.
Dot blot analysis of pan NOS using Anti-pan NOS antibody [RM1126] ab316334 at 1:1000 (0.503 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1:20,000 dilution.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] (Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] ab205606) as total protein control staining at 1/1000 dilution.
All lanes: Dot Blot - Anti-pan NOS antibody [RM1126] (Anti-pan NOS antibody [RM1126] ab316334) at 1/1000 dilution
Lane 1: C-Myc/DDK tagged full length human nNOS recombinant protein
Lane 2: C-Myc/DDK tagged full length human iNOS recombinant protein
Lane 3: C-Myc/DDK tagged full length human eNOS recombinant protein
SecondaryAll lanes: Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Exposure time: 1s
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan NOS antibody [RM1126] - BSA and Azide free (ab316335), expandable thumbnail](https://content.abcam.com/products/images/pan-nos-antibody-rm1126-bsa-and-azide-free-ab316335--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img432266.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan NOS antibody [RM1126] - BSA and Azide free (ab316335)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan NOS antibody [RM1126] - BSA and Azide free (ab316335)
This data was developed using Anti-pan NOS antibody [RM1126] ab316334, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded (A) HEK-293T transfected with a human nNOS expression vector containing a Myc-His tag, (B) HEK-293T transfected with a human iNOS expression vector containing a Myc-His tag, and (C) HEK-293T transfected with a human eNOS expression vector containing a Myc-His tag, no staining on (D) HEK-293T transfected with empty vector containing a Myc-His tag tissues labeling pan NOS with Anti-pan NOS antibody [RM1126] ab316334 at 1/2000 (0.252 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Positive staining on (A) HEK-293T transfected with a human nNOS expression vector containing a Myc-His tag, (B) HEK-293T transfected with a human iNOS expression vector containing a Myc-His tag, and (C) HEK-293T transfected with a human eNOS expression vector containing a Myc-His tag, no staining on (D) HEK-293T transfected with empty vector containing a Myc-His tag. The section was incubated with Anti-pan NOS antibody [RM1126] ab316334 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan NOS antibody [RM1126] - BSA and Azide free (ab316335), expandable thumbnail](https://content.abcam.com/products/images/pan-nos-antibody-rm1126-bsa-and-azide-free-ab316335--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img432260.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan NOS antibody [RM1126] - BSA and Azide free (ab316335)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan NOS antibody [RM1126] - BSA and Azide free (ab316335)
This data was developed using Anti-pan NOS antibody [RM1126] ab316334, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human cerebellum tissue labeling pan NOS with Anti-pan NOS antibody [RM1126] ab316334 at 1/200 (2.515 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Positive staining on human cerebellum. The section was incubated with Anti-pan NOS antibody [RM1126] ab316334 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan NOS antibody [RM1126] - BSA and Azide free (ab316335), expandable thumbnail](https://content.abcam.com/products/images/pan-nos-antibody-rm1126-bsa-and-azide-free-ab316335--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img432263.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan NOS antibody [RM1126] - BSA and Azide free (ab316335)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan NOS antibody [RM1126] - BSA and Azide free (ab316335)
This data was developed using Anti-pan NOS antibody [RM1126] ab316334, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling pan NOS with Anti-pan NOS antibody [RM1126] ab316334 at 1/200 (2.515 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Positive staining on endothelial cells in human spleen. The section was incubated with Anti-pan NOS antibody [RM1126] ab316334 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan NOS antibody [RM1126] - BSA and Azide free (ab316335), expandable thumbnail](https://content.abcam.com/products/images/pan-nos-antibody-rm1126-bsa-and-azide-free-ab316335--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img432262.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan NOS antibody [RM1126] - BSA and Azide free (ab316335)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan NOS antibody [RM1126] - BSA and Azide free (ab316335)
This data was developed using Anti-pan NOS antibody [RM1126] ab316334, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling pan NOS with Anti-pan NOS antibody [RM1126] ab316334 at 1/200 (2.515 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Positive staining on human lung. The section was incubated with Anti-pan NOS antibody [RM1126] ab316334 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan NOS antibody [RM1126] - BSA and Azide free (ab316335), expandable thumbnail](https://content.abcam.com/products/images/pan-nos-antibody-rm1126-bsa-and-azide-free-ab316335--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img432265.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan NOS antibody [RM1126] - BSA and Azide free (ab316335)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan NOS antibody [RM1126] - BSA and Azide free (ab316335)
This data was developed using Anti-pan NOS antibody [RM1126] ab316334, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human cervical squamous carcinoma tissue labeling pan NOS with Anti-pan NOS antibody [RM1126] ab316334 at 1/200 (2.515 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Positive staining on endothelial cells in human cervical squamous carcinoma. The section was incubated with Anti-pan NOS antibody [RM1126] ab316334 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan NOS antibody [RM1126] - BSA and Azide free (ab316335), expandable thumbnail](https://content.abcam.com/products/images/pan-nos-antibody-rm1126-bsa-and-azide-free-ab316335--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img432264.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan NOS antibody [RM1126] - BSA and Azide free (ab316335)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan NOS antibody [RM1126] - BSA and Azide free (ab316335)
This data was developed using Anti-pan NOS antibody [RM1126] ab316334, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling pan NOS with Anti-pan NOS antibody [RM1126] ab316334 at 1/200 (2.515 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Positive staining on human placenta. The section was incubated with Anti-pan NOS antibody [RM1126] ab316334 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan NOS antibody [RM1126] - BSA and Azide free (ab316335), expandable thumbnail](https://content.abcam.com/products/images/pan-nos-antibody-rm1126-bsa-and-azide-free-ab316335--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img432259.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan NOS antibody [RM1126] - BSA and Azide free (ab316335)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan NOS antibody [RM1126] - BSA and Azide free (ab316335)
This data was developed using Anti-pan NOS antibody [RM1126] ab316334, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human thalamus tissue labeling pan NOS with Anti-pan NOS antibody [RM1126] ab316334 at 1/200 (2.515 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Positive staining on human thalamus. The section was incubated with Anti-pan NOS antibody [RM1126] ab316334 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan NOS antibody [RM1126] - BSA and Azide free (ab316335), expandable thumbnail](https://content.abcam.com/products/images/pan-nos-antibody-rm1126-bsa-and-azide-free-ab316335--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img432261.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan NOS antibody [RM1126] - BSA and Azide free (ab316335)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan NOS antibody [RM1126] - BSA and Azide free (ab316335)
This data was developed using Anti-pan NOS antibody [RM1126] ab316334, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling pan NOS with Anti-pan NOS antibody [RM1126] ab316334 at 1/200 (2.515 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Positive staining on human liver. The section was incubated with Anti-pan NOS antibody [RM1126] ab316334 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan NOS antibody [RM1126] - BSA and Azide free (ab316335), expandable thumbnail](https://content.abcam.com/products/images/pan-nos-antibody-rm1126-bsa-and-azide-free-ab316335--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img432265.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan NOS antibody [RM1126] - BSA and Azide free (ab316335)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan NOS antibody [RM1126] - BSA and Azide free (ab316335), expandable thumbnail](https://content.abcam.com/products/images/pan-nos-antibody-rm1126-bsa-and-azide-free-ab316335--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img432264.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan NOS antibody [RM1126] - BSA and Azide free (ab316335)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan NOS antibody [RM1126] - BSA and Azide free (ab316335), expandable thumbnail](https://content.abcam.com/products/images/pan-nos-antibody-rm1126-bsa-and-azide-free-ab316335--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img432259.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan NOS antibody [RM1126] - BSA and Azide free (ab316335)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan NOS antibody [RM1126] - BSA and Azide free (ab316335), expandable thumbnail](https://content.abcam.com/products/images/pan-nos-antibody-rm1126-bsa-and-azide-free-ab316335--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img432261.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan NOS antibody [RM1126] - BSA and Azide free (ab316335)")
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