Anti-pan PDK antibody [3H5BE12]

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan PDK antibody [3H5BE12] (AB115321)

IHC image of ab115321 staining in human skeletal muscle formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab115321, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

• WB

AbReview43642****

Western blot - Anti-pan PDK antibody [3H5BE12] (AB115321)

Western blot analysis of human ovarian cancer cell lines whole cell lysate (30μg/lane) labelling pan PDK with ab115321 at 1/1000. A HRP-conjugated goat anti-mouse IgG polyclonal (1/10000) was used as the secondary antibody.

All lanes:

Western blot - Anti-pan PDK antibody [3H5BE12] (ab115321)

Predicted band size: 49 kDa

Observed band size: 49 kDa

true

Exposure time: 10s

This image is courtesy of an anonymous Abreview

• WB

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Western blot - Anti-pan PDK antibody [3H5BE12] (AB115321)

ab115321 identifies all four isoforms of PDKs (PDK1, PDK2, PDK3, and PDK4) with differential affinity. PDK3 has the highest affinity, then PDK2 and 4, it has the lowest affinity to PDK1.

All lanes:

Western blot - Anti-pan PDK antibody [3H5BE12] (ab115321) at 1 µg/mL

Lane 1:

human heart mitochondria at 20 µg

Lane 2:

human liver mitochondria at 10 µg

Lane 3:

rat liver mitochondria at 10 µg

Lane 4:

mouse liver mitochondria at 10 µg

Lane 5:

PDK1 recombinant protein <a href='/en-us/products/unavailable/recombinant-human-pdk1-protein-ab110359'>ab110359</a> at 0.1 µg

Lane 6:

PDK2 recombinant protein <a href='/en-us/products/unavailable/recombinant-human-pdk2-protein-ab110354'>ab110354</a> at 0.1 µg

Lane 7:

PDK3 recombinant protein <a href='/en-us/products/unavailable/recombinant-human-pdk3-protein-ab110355'>ab110355</a> at 0.1 µg

Lane 8:

PDK4 recombinant protein <a href='/en-us/products/unavailable/recombinant-human-pdk4-protein-ab110356'>ab110356</a> at 0.1 µg

Lane 9:

PDK1 recombinant protein <a href='/en-us/products/proteins-peptides/recombinant-human-pdk1-protein-ab86986'>ab86986</a> at 0.1 µg

Secondary

All lanes:

GAM IR 800 1:5000

Predicted band size: 49 kDa

false