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Mouse Monoclonal PDK1 antibody. Suitable for IHC-P, WB and reacts with Human, Rat, Mouse, Recombinant full length protein - Human samples. Cited in 3 publications.


Images

Western blot - Anti-pan PDK antibody [3H5BE12] (AB115321), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan PDK antibody [3H5BE12] (AB115321), expandable thumbnail
  • Western blot - Anti-pan PDK antibody [3H5BE12] (AB115321), expandable thumbnail

Publications

Key facts

Isotype
IgG2b
Host species
Mouse
Storage buffer

pH: 7.5
Preservative: 0.02% Sodium azide
Constituents: HEPES buffered saline

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWB
Human
Tested
Tested
Mouse
Expected
Tested
Rat
Expected
Tested
Recombinant full length protein - Human
Not recommended
Tested

Tested
Tested

Species
Human
Dilution info
5 µg/mL
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Expected
Expected

Species
Rat, Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Recombinant full length protein - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Recombinant full length protein - Human
Dilution info
1 µg/mL
Notes

-

Species
Rat
Dilution info
1 µg/mL
Notes

-

Species
Human
Dilution info
1 µg/mL
Notes

-

Species
Mouse
Dilution info
1 µg/mL
Notes

-

Target data

Function

Kinase that plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Plays an important role in cellular responses to hypoxia and is important for cell proliferation under hypoxia. Protects cells against apoptosis in response to hypoxia and oxidative stress.

Alternative names

PDHK1, PDK1, Pyruvate dehydrogenase kinase isoform 1, PDH kinase 1

Recommended products

Mouse Monoclonal PDK1 antibody. Suitable for IHC-P, WB and reacts with Human, Rat, Mouse, Recombinant full length protein - Human samples. Cited in 3 publications.

Key facts

Isotype
IgG2b
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
3H5BE12
Purification technique
Proprietary technique
Light chain type
kappa
Concentration
Loading...
Purification notes

Near homogeneity as judged by SDS-PAGE (>95% purity). The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

PDH Kinases (PDKs) deactivate PDH by reversible phosphorylation. In humans and rodents, 4 PDK's are present in an organ-dependent manner. In all cases, PDK's are physically part of the PDH complex (binding via lipoyl domain 2 of E2), and they phosphorylate the E1 subunit at 3 specific serine residues. PDH phosphatases remove the phosphorylation and restore activity of PDH.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Pan PDK or pyruvate dehydrogenase kinases are a family of enzymes that regulate glucose metabolism by inhibiting the pyruvate dehydrogenase complex (PDC). The four human PDK isoforms are known: PDK1 PDK2 PDK3 and PDK4. These kinases typically express in tissues like heart liver muscle and kidney where they play important roles in energy metabolism. The molecular weights of PDKs range from around 40 to 49 kDa.

Biological function summary

Pyruvate dehydrogenase kinases control the flux between glycolysis and the tricarboxylic acid cycle. By phosphorylating the E1α subunit of PDC PDKs decrease PDC activity slowing down the conversion of pyruvate into acetyl-CoA. In this way PDKs help balance energy production especially in tissues requiring flexible metabolic responses. This function connects them to energy homeostasis especially during starvation and exercise where fatty acids serve as the primary energy source.

Pathways

PDKs are central in key metabolic pathways like glycolysis and the tricarboxylic acid cycle. These pathways ensure energy production efficiency based on cellular needs. Through their action PDKs interact with proteins like the E2 and E3 components of PDC modifying their activities according to cellular conditions. The regulation of PDKs also involves signaling pathways affecting glucose and fat metabolism which makes them integral to broader metabolic control mechanisms.

Associated diseases and disorders

Imbalances in PDK activity link to metabolic syndromes such as diabetes and cancer. In diabetes altered PDK expression can contribute to impaired glucose utilization impacting insulin sensitivity. In some cancers upregulated PDKs promote aerobic glycolysis known as the Warburg effect supporting rapid tumor growth. Proteins involved in insulin signaling pathways and oncogenes are often connected to PDKs in these contexts highlighting their role in disease progression and potential as therapeutic targets.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-pan PDK antibody [3H5BE12] (ab115321), expandable thumbnail

    Western blot - Anti-pan PDK antibody [3H5BE12] (ab115321)

    ab115321 identifies all four isoforms of PDKs (PDK1, PDK2, PDK3, and PDK4) with differential affinity. PDK3 has the highest affinity, then PDK2 and 4, it has the lowest affinity to PDK1.

    All lanes: Western blot - Anti-pan PDK antibody [3H5BE12] (ab115321) at 1 µg/mL

    Lane 1: human heart mitochondria at 20 µg

    Lane 2: human liver mitochondria at 10 µg

    Lane 3: rat liver mitochondria at 10 µg

    Lane 4: mouse liver mitochondria at 10 µg

    Lane 5: PDK1 recombinant protein ab110359 at 0.1 µg

    Lane 6: PDK2 recombinant protein ab110354 at 0.1 µg

    Lane 7: PDK3 recombinant protein ab110355 at 0.1 µg

    Lane 8: PDK4 recombinant protein ab110356 at 0.1 µg

    Lane 9: PDK1 recombinant protein Recombinant Human PDK1 protein ab86986 at 0.1 µg

    Secondary

    All lanes: GAM IR 800 1:5000

    Performed under reducing conditions.

    Predicted band size: 49 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan PDK antibody [3H5BE12] (ab115321), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan PDK antibody [3H5BE12] (ab115321)

    IHC image of ab115321 staining in human skeletal muscle formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab115321, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.



    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Western blot - Anti-pan PDK antibody [3H5BE12] (ab115321), expandable thumbnail
    This image is courtesy of an anonymous customer review

    Western blot - Anti-pan PDK antibody [3H5BE12] (ab115321)

    Western blot analysis of human ovarian cancer cell lines whole cell lysate (30μg/lane) labelling pan PDK with ab115321 at 1/1000. A HRP-conjugated goat anti-mouse IgG polyclonal (1/10000) was used as the secondary antibody.

    All lanes: Western blot - Anti-pan PDK antibody [3H5BE12] (ab115321)

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 49 kDa

    Observed band size: 49 kDa

    Exposure time: 10s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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