AB6176

Anti-pan Synuclein antibody

Name: Anti-pan Synuclein antibody (ab6176) | Abcam
Brand: Abcam Limited
SKU: AB6176
Price: 535 USD
Availability: InStock
Rating: 4 (4 reviews)

(4 Reviews)

(18 Publications)

Rabbit Polyclonal Alpha-synuclein antibody. Suitable for WB, IHC-P, ICC/IF, IHC-Fr and reacts with Rat, Human samples. Cited in 18 publications. Immunogen corresponding to Synthetic Peptide within Human SNCA aa 1-50.

View Alternative Names

NACP, PARK1, SNCA, Alpha-synuclein, Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor

1 Images

Immunocytochemistry/ Immunofluorescence - Anti-pan Synuclein antibody (AB6176)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-pan Synuclein antibody (AB6176)

ICC/IF image of ab6176 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6176, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Rat

Applications

WB, IHC-Fr, IHC-P, ICC/IF

applications

Immunogen

Synthetic Peptide within Human SNCA aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

P37840

Specificity

This antibody recognises all 3 forms of the synucleins.

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Reactivity data

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Properties and storage information

Form

Liquid

Purity

Whole antiserum

Storage buffer

pH: 7 - 7.4

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Add glycerol to a final volume of 50% for extra stability and aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Pan Synuclein also known as alpha beta and gamma synucleins are small proteins with molecular masses around 14-19 kDa. These proteins are expressed largely in the central nervous system and are also present in other tissues such as the peripheral nervous system and certain types of muscles. They share structural similarities and comprise a family often involved with neurons where they play roles in various cellular processes.

Biological function summary

Synucleins participate in the regulation of synaptic function and plasticity influencing neurotransmitter release and membrane dynamics. They do not form a specific complex but can interact with lipid membranes and various cellular proteins affecting vesicular transport processes. These proteins hold influence over essential cellular functions that depend on synaptic activity and efficiency.

Pathways

Synucleins involve themselves in important neuronal pathways such as those controlling dopamine neurotransmission and regulation of mitochondrial functions. They interact with key proteins like parkin and ubiquitin which participate in protein degradation pathways. Synucleins' roles in these pathways highlight their impact on both synaptic transmission and energy metabolism within neurons.

Synucleins have strong connections to neurodegenerative disorders like Parkinson's disease and dementia with Lewy bodies. In these disorders alpha-synuclein in particular forms aggregates known as Lewy bodies contributing to neuronal dysfunction. These aggregates interact with proteins such as tau and amyloid-beta further linking synucleins to the pathology of these disorders. Understanding these interactions provides insights into potential targets for therapeutic interventions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

The protein expressed by the SNCA gene is involved in various synaptic activities, including the regulation of synaptic vesicle trafficking and neurotransmitter release. As a monomer, it enhances synaptic vesicle exocytosis through vesicle priming, fusion, and dilation of exocytotic fusion pores. Mechanistically, it increases local Ca(2+) release, which is crucial for ATP-induced exocytosis. In its multimeric membrane-bound form, SNCA acts as a molecular chaperone, assisting in the folding of synaptic fusion components known as SNAREs at the presynaptic plasma membrane, in conjunction with cysteine string protein-alpha/DNAJC5, a function that is vital for maintaining normal SNARE-complex assembly during aging. Additionally, SNCA plays a role in dopamine neurotransmission regulation by associating with the dopamine transporter (DAT1) and modulating its activity. This supplementary information is collated from multiple sources and compiled automatically.

See full target information SNCA

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Publications (18)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 300:107419 PubMed38815862

2024

Neuronal activity promotes secretory autophagy for the extracellular release of α-synuclein.

Applications

Unspecified application

Species

Unspecified reactive species

Yoshitsugu Nakamura,Taiki Sawai,Kensuke Kakiuchi,Shigeki Arawaka

Frontiers in cell and developmental biology 9:774650 PubMed34901020

2021

Synuclein Regulates Synaptic Vesicle Clustering and Docking at a Vertebrate Synapse.

Applications

Unspecified application

Species

Unspecified reactive species

Kaitlyn E Fouke,M Elizabeth Wegman,Sarah A Weber,Emily B Brady,Cristina Román-Vendrell,Jennifer R Morgan

Neurobiology of disease 151:105256 PubMed33429042

2021

Doxycycline inhibits α-synuclein-associated pathologies in vitro and in vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Antonio Dominguez-Meijide,Valeria Parrales,Eftychia Vasili,Florencia González-Lizárraga,Annekatrin König,Diana F Lázaro,Annie Lannuzel,Stéphane Haik,Elaine Del Bel,Rosana Chehín,Rita Raisman-Vozari,Patrick P Michel,Nicolas Bizat,Tiago Fleming Outeiro

Journal of neurochemistry 156:674-691 PubMed32730640

2020

Dopamine promotes the neurodegenerative potential of β-synuclein.

Applications

Unspecified application

Species

Unspecified reactive species

Anupam Raina,Kristian Leite,Sofia Guerin,Sameehan U Mahajani,Kalyan S Chakrabarti,Diana Voll,Stefan Becker,Christian Griesinger,Mathias Bähr,Sebastian Kügler

Cell death & disease 10:898 PubMed31776327

2019

Homogenous generation of dopaminergic neurons from multiple hiPSC lines by transient expression of transcription factors.

Applications

Unspecified application

Species

Unspecified reactive species

Sameehan Mahajani,Anupam Raina,Claudia Fokken,Sebastian Kügler,Mathias Bähr

The Journal of biological chemistry 294:9973-9984 PubMed31092553

2019

C-terminal α-synuclein truncations are linked to cysteine cathepsin activity in Parkinson's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Ryan P McGlinchey,Shannon M Lacy,Katherine E Huffer,Nahid Tayebi,Ellen Sidransky,Jennifer C Lee

Neurobiology of disease 124:276-288 PubMed30381260

2018

Development of an aggregate-selective, human-derived α-synuclein antibody BIIB054 that ameliorates disease phenotypes in Parkinson's disease models.

Applications

Unspecified application

Species

Unspecified reactive species

Andreas Weihofen,YuTing Liu,Joseph W Arndt,Christian Huy,Chao Quan,Benjamin A Smith,Jean-Luc Baeriswyl,Nicole Cavegn,Luzia Senn,Lihe Su,Galina Marsh,Pavan K Auluck,Fabio Montrasio,Roger M Nitsch,Warren D Hirst,Jesse M Cedarbaum,R Blake Pepinsky,Jan Grimm,Paul H Weinreb

PloS one 12:e0184262 PubMed28877262

2017

The ER retention protein RER1 promotes alpha-synuclein degradation via the proteasome.

Applications

Unspecified application

Species

Unspecified reactive species

Hyo-Jin Park,Daniel Ryu,Mayur Parmar,Benoit I Giasson,Nikolaus R McFarland

Human molecular genetics 25:275-90 PubMed26586132

2015

Yeast reveals similar molecular mechanisms underlying alpha- and beta-synuclein toxicity.

Applications

ICC/IF

Species

Human

Sandra Tenreiro,Rita Rosado-Ramos,Ellen Gerhardt,Filippo Favretto,Filipa Magalhães,Blagovesta Popova,Stefan Becker,Markus Zweckstetter,Gerhard H Braus,Tiago Fleming Outeiro

Journal of neuropathology and experimental neurology 72:624-45 PubMed23771222

2013

α-Synuclein vaccination prevents the accumulation of parkinson disease-like pathologic inclusions in striatum in association with regulatory T cell recruitment in a rat model.

Applications

Unspecified application

Species

Unspecified reactive species

Vanesa Sanchez-Guajardo,Ambra Annibali,Poul Henning Jensen,Marina Romero-Ramos

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-pan Synuclein antibody

Immunocytochemistry/ Immunofluorescence - Anti-pan Synuclein antibody (AB6176)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (18)

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