AB197589

Anti-PAN TEAD antibody [EPR15629]

RabMAb
Recombinant
What is this?

(1 Review)

(11 Publications)

Rabbit Recombinant Monoclonal TEAD4 antibody. Suitable for WB and reacts with Human samples. Cited in 11 publications.

View Alternative Names

RTEF1, TCF13L1, TEF3, TEAD4, Transcriptional enhancer factor TEF-3, TEA domain family member 4, Transcription factor 13-like 1, Transcription factor RTEF-1, TEAD-4

3 Images

Supplier Data

Western blot - Anti-PAN TEAD antibody [EPR15629] (AB197589)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-PAN TEAD antibody [EPR15629] (ab197589) at 1/1000 dilution

Lane 1:

HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 2:

SW480 (Human colorectal adenocarcinoma cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 48 kDa

Observed band size: 54 kDa

false

Exposure time: 1min

Lab

Western blot - Anti-PAN TEAD antibody [EPR15629] (AB197589)

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-PAN TEAD antibody [EPR15629] (ab197589) at 1/1000 dilution

Lane 1:

TEAD1 Human Recombinant Protein at 20 µg

Lane 2:

TEAD2 Human Recombinant Protein at 20 µg

Lane 3:

TEAD3 Human Recombinant Protein at 20 µg

Lane 4:

TEAD4 Human Recombinant Protein at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 50-75 kDa

false

Exposure time: 180s

Supplier Data

Western blot - Anti-PAN TEAD antibody [EPR15629] (AB197589)

5% NFDM/TBST : Blocking and diluting buffer.

All lanes:

Western blot - Anti-PAN TEAD antibody [EPR15629] (ab197589) at 1/5000 dilution

Lane 1:

Human TEAD4 recombinant protein (GST-Tag) aa165-280 at 10 µg

Lane 2:

Human TEAD1 recombinant protein (GST-Tag) aa161-280 at 10 µg

Lane 3:

Human TEAD2 recombinant protein (GST-Tag) aa161-290 at 10 µg

Lane 4:

Human TEAD3 recombinant protein (GST-Tag) aa161-290 at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 48 kDa

Observed band size: 39 kDa

false

Exposure time: 5s

Carrier free

Anti-PAN TEAD antibody [EPR15629] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15629

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Mouse": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Acts by mediating gene expression of YAP1 and WWTR1/TAZ, thereby regulating cell proliferation, migration and epithelial mesenchymal transition (EMT) induction. Binds specifically and non-cooperatively to the Sph and GT-IIC 'enhansons' (5'-GTGGAATGT-3') and activates transcription. Binds to the M-CAT motif.

See full target information TEAD4

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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

Journal for immunotherapy of cancer 13: PubMed40789740

2025

Overcoming immunotherapy resistance in bladder cancer with a novel antibody-drug conjugate RC48.

Applications

Unspecified application

Species

Unspecified reactive species

Jiatong Xiao,Jinhui Liu,Chunyu Zhang,Zhi Liu,Zhenyu Nie,Zhenglin Yi,Xin Gao,Haisu Liang,Jinliang Huang,Zhiyong Cai,Luzhe Yan,Bingquan Wu,Zefu Liu,Jinbo Chen,Xiongbing Zu,Jiao Hu

Nature communications 16:4128 PubMed40319089

2025

Harnessing the FGFR2/NF2/YAP signaling-dependent necroptosis to develop an FGFR2/IL-8 dual blockade therapeutic strategy.

Applications

Unspecified application

Species

Unspecified reactive species

Dongshao Chen,Zitong Zhao,Ruoxi Hong,Di Yang,Ying Gong,Qingnan Wu,Yan Wang,Yiren Cao,Jie Chen,Yidi Tai,Haoyu Liu,Jinting Li,Jiawen Fan,Weimin Zhang,Yongmei Song,Qimin Zhan

Signal transduction and targeted therapy 8:302 PubMed37582812

2023

CDK7-YAP-LDHD axis promotes D-lactate elimination and ferroptosis defense to support cancer stem cell-like properties.

Applications

Unspecified application

Species

Unspecified reactive species

Mengzhu Lv,Ying Gong,Xuesong Liu,Yan Wang,Qingnan Wu,Jie Chen,Qingjie Min,Dongyu Zhao,Xianfeng Li,Dongshao Chen,Di Yang,Danna Yeerken,Rui Liu,Jinting Li,Weimin Zhang,Qimin Zhan

Nature communications 14:2253 PubMed37080959

2023

LncRNA modulates Hippo-YAP signaling to reprogram iron metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Xin-Yu He,Xiao Fan,Lei Qu,Xiang Wang,Li Jiang,Ling-Jie Sang,Cheng-Yu Shi,Siyi Lin,Jie-Cheng Yang,Zuo-Zhen Yang,Kai Lei,Jun-Hong Li,Huai-Qiang Ju,Qingfeng Yan,Jian Liu,Fudi Wang,Jianzhong Shao,Yan Xiong,Wenqi Wang,Aifu Lin

Cell death discovery 8:59 PubMed35149670

2022

YAP ISGylation increases its stability and promotes its positive regulation on PPP by stimulating 6PGL transcription.

Applications

Unspecified application

Species

Unspecified reactive species

Xiangfei Xue,Xiaoting Tian,Congcong Zhang,Yayou Miao,Yikun Wang,Yingxiu Peng,Shiyu Qiu,Hong Wang,Jiangtao Cui,Leiqun Cao,Fenyong Sun,Yongxia Qiao,Xiao Zhang

Calcified tissue international 109:203-214 PubMed33713163

2021

RTEF-1 Inhibits Vascular Smooth Muscle Cell Calcification through Regulating Wnt/β-Catenin Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jingjing Cong,Bei Cheng,Jinyu Liu,Ping He

Journal of hematology & oncology 13:77 PubMed32546241

2020

Regulation of Hippo-YAP signaling by insulin-like growth factor-1 receptor in the tumorigenesis of diffuse large B-cell lymphoma.

Applications

Unspecified application

Species

Unspecified reactive species

Xiangxiang Zhou,Na Chen,Hongzhi Xu,Xiaoming Zhou,Jianhong Wang,Xiaosheng Fang,Ya Zhang,Ying Li,Juan Yang,Xin Wang

International journal of oncology 56:232-242 PubMed31789387

2019

Interaction of YAP1 and mTOR promotes bladder cancer progression.

Applications

Unspecified application

Species

Unspecified reactive species

Mingxi Xu,Meng Gu,Juan Zhou,Jun Da,Zhong Wang

Cellular and molecular life sciences : CMLS 77:1135-1152 PubMed31321444

2019

ZNFX1 anti-sense RNA 1 promotes the tumorigenesis of prostate cancer by regulating c-Myc expression via a regulatory network of competing endogenous RNAs.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaolu Cui,Chiyuan Piao,Chengcheng Lv,Xuyong Lin,Zhe Zhang,Xiankui Liu

The Biochemical journal 475:2955-2967 PubMed30120107

2018

IFN-γ-response mediator GBP-1 represses human cell proliferation by inhibiting the Hippo signaling transcription factor TEAD.

Applications

Unspecified application

Species

Unspecified reactive species

Bea Unterer,Veit Wiesmann,Mekala Gunasekaran,Heinrich Sticht,Clara Tenkerian,Jürgen Behrens,Marina Leone,Felix B Engel,Nathalie Britzen-Laurent,Elisabeth Naschberger,Thomas Wittenberg,Michael Stürzl

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PAN TEAD antibody [EPR15629]

Western blot - Anti-PAN TEAD antibody [EPR15629] (AB197589)

Western blot - Anti-PAN TEAD antibody [EPR15629] (AB197589)

Western blot - Anti-PAN TEAD antibody [EPR15629] (AB197589)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (11)

Product promise