Rabbit Recombinant Monoclonal TEAD4 antibody. Carrier free. Suitable for WB and reacts with Human samples.
Images
![Western blot - Anti-PAN TEAD antibody [EPR15629] - BSA and Azide free (AB240976), expandable thumbnail](https://content.abcam.com/products/images/pan-tead-antibody-epr15629-bsa-and-azide-free-ab240976--western-blot-img6549.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PAN TEAD antibody [EPR15629] - BSA and Azide free (AB240976)")
![Western blot - Anti-PAN TEAD antibody [EPR15629] - BSA and Azide free (AB240976), expandable thumbnail](https://content.abcam.com/products/images/pan-tead-antibody-epr15629-bsa-and-azide-free-ab240976--western-blot-img424985.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PAN TEAD antibody [EPR15629] - BSA and Azide free (AB240976)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | ICC/IF | IP | |
|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
Target data
Function
Transcription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Acts by mediating gene expression of YAP1 and WWTR1/TAZ, thereby regulating cell proliferation, migration and epithelial mesenchymal transition (EMT) induction. Binds specifically and non-cooperatively to the Sph and GT-IIC 'enhansons' (5'-GTGGAATGT-3') and activates transcription. Binds to the M-CAT motif.
Alternative names
RTEF1, TCF13L1, TEF3, TEAD4, Transcriptional enhancer factor TEF-3, TEA domain family member 4, Transcription factor 13-like 1, Transcription factor RTEF-1, TEAD-4
Recommended products
Rabbit Recombinant Monoclonal TEAD4 antibody. Carrier free. Suitable for WB and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR15629
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab240976 is the carrier-free version of Anti-PAN TEAD antibody [EPR15629] ab197589.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-PAN TEAD antibody [EPR15629] - BSA and Azide free (ab240976), expandable thumbnail](https://content.abcam.com/products/images/pan-tead-antibody-epr15629-bsa-and-azide-free-ab240976--western-blot-img6549.jpg "Western blot - Anti-PAN TEAD antibody [EPR15629] - BSA and Azide free (ab240976)")
Western blot - Anti-PAN TEAD antibody [EPR15629] - BSA and Azide free (ab240976)
Blocking/Dilution buffer: 5% NFDM/TBST.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PAN TEAD antibody [EPR15629] ab197589).
All lanes: Western blot - Anti-PAN TEAD antibody [EPR15629] (Anti-PAN TEAD antibody [EPR15629] ab197589) at 1/1000 dilution
Lane 1: HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 20 µg
Lane 2: SW480 (Human colorectal adenocarcinoma cell line) whole cell lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 48 kDa
Observed band size: 54 kDa
Exposure time: 1min
![Western blot - Anti-PAN TEAD antibody [EPR15629] - BSA and Azide free (ab240976), expandable thumbnail](https://content.abcam.com/products/images/pan-tead-antibody-epr15629-bsa-and-azide-free-ab240976--western-blot-img424985.jpg "Western blot - Anti-PAN TEAD antibody [EPR15629] - BSA and Azide free (ab240976)")
Western blot - Anti-PAN TEAD antibody [EPR15629] - BSA and Azide free (ab240976)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PAN TEAD antibody [EPR15629] ab197589).
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-PAN TEAD antibody [EPR15629] (Anti-PAN TEAD antibody [EPR15629] ab197589) at 1/1000 dilution
Lane 1: TEAD1 Human Recombinant Protein at 20 µg
Lane 2: TEAD2 Human Recombinant Protein at 20 µg
Lane 3: TEAD3 Human Recombinant Protein at 20 µg
Lane 4: TEAD4 Human Recombinant Protein at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 50-75 kDa
Exposure time: 180s
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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