AB246551

Anti-Pan Trk antibody [EPR17341-4]

RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(2 Publications)

Rabbit Recombinant Monoclonal TrkB antibody. Suitable for IP, WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 2 publications.

View Alternative Names

TRKB, NTRK2, BDNF/NT-3 growth factors receptor, GP145-TrkB, Neurotrophic tyrosine kinase receptor type 2, TrkB tyrosine kinase, Tropomyosin-related kinase B, Trk-B

8 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pan Trk antibody [EPR17341-4] (AB246551)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pan Trk antibody [EPR17341-4] (AB246551)

Immunohistochemical analysis of paraffin-embedded Human astrocytoma labelling Pan Trk with ab246551 at 1/500 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP polymer) ready to use (ab214880). Positive staining on human astrocytoma is observed. Counter stained with hematoxylin. Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a Goat Anti-Rabbit IgG H&L (HRP polymer) ready to use. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pan Trk antibody [EPR17341-4] (AB246551)

Immunohistochemical analysis of paraffin-embedded human cerebral cortex tissue labeling Pan Trk with ab246551 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic staining in neuron of human cerebral cortex (PMID : 7936202) is observed. Counter stained with hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pan Trk antibody [EPR17341-4] (AB246551)

Immunohistochemical analysis of paraffin-embedded human liver tissue labeling Pan Trk with ab246551 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Negative staining is observed in normal human liver. Counterstained with hematoxylin.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Unknown

Immunoprecipitation - Anti-Pan Trk antibody [EPR17341-4] (AB246551)

Pan Trk was immunoprecipitated from 0.35 mg of human fetal brain lysate with ab246551 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab246551 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.

Lane 1 : Human fetal brain lysate 10 μg (Input).
Lane 2 : ab246551 IP in human fetal brain lysate (+).
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab246551 in human fetal brain lysate (-).

Blocking and dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 10 seconds.

The 30kDa band is an intracellular fragment TrkB-ICD, and the 140KDa observed MW is due to the glycosylation modification (PMID : 24860020).

All lanes:

Immunoprecipitation - Anti-Pan Trk antibody [EPR17341-4] (ab246551)

Observed band size: 140 kDa,30 kDa

false

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pan Trk antibody [EPR17341-4] (AB246551)

Immunohistochemical analysis of paraffin-embedded mouse cerebral cortex tissue labeling Pan Trk with ab246551 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic staining in neuron of mouse cerebral cortex (PMID : 2171894) is observed. Counter stained with hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pan Trk antibody [EPR17341-4] (AB246551)

Immunohistochemical analysis of paraffin-embedded rat cerebral cortex tissue labeling Pan Trk with ab246551 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic staining in neuron of rat cerebral cortex is observed. Counter stained with hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Unknown

Western blot - Anti-Pan Trk antibody [EPR17341-4] (AB246551)

Blocking and dilution buffer : 5% NFDM/TBST.

The 30kDa band is an intracellular fragment TrkB-ICD, and the 140KDa observed MW is due to the glycosylation modification (PMID : 24860020).

Negative control : human fetal heart, human fetal kidney, human fetal spleen.

All lanes:

Western blot - Anti-Pan Trk antibody [EPR17341-4] (ab246551) at 1/1000 dilution

Lane 1:

Human fetal brain lysate at 10 µg

Lane 2:

Human fetal heart lysate at 10 µg

Lane 3:

Human fetal kidney lysate at 10 µg

Lane 4:

Human fetal spleen lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 92 kDa

Observed band size: 140 kDa,30 kDa

false

Exposure time: 3min

Unknown

Western blot - Anti-Pan Trk antibody [EPR17341-4] (AB246551)

Exposure time : Lanes 1 and 2 : 3 minutes; Lane 3 : 3 seconds.

Blocking and dilution buffer : 5% NFDM/TBST.

The 30kDa band is an intracellular fragment TrkB-ICD, and the 140KDa observed MW is due to the glycosylation modification (PMID : 24860020).

All lanes:

Western blot - Anti-Pan Trk antibody [EPR17341-4] (ab246551) at 1/1000 dilution

Lane 1:

Rat brain lysate at 20 µg

Lane 2:

Human hippocampus lysate at 20 µg

Lane 3:

Mouse brain lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 92 kDa

Observed band size: 140 kDa,30 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR17341-4

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IHC-P, IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" } } }

style

left-column

Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Pan Trk also known as Pan-tropomyosin receptor kinases are receptors that belong to the neurotrophin receptor family including TrkA TrkB and TrkC. They typically express in neural tissues and play a role in neuronal development and function. The mass of Pan Trk can vary as it encompasses several related proteins rather than a single unit. The variant forms are present in both the central and peripheral nervous systems. Researchers often use tags like Pan Trk IHc 555 Pan and Alexa Pan to identify different forms and monitor their expression patterns.

Biological function summary

Pan Trk receptors play a vital role in the survival development and function of neurons by mediating the action of neurotrophins. They participate as part of a receptor complex activated by ligand binding which triggers autophosphorylation. These receptors modulate various processes like neuronal survival and differentiation. Pan Trk signals generally initiate upon binding neurotrophins leading to multiple intracellular events.

Pathways

Pan Trk receptors are central players in signaling pathways such as the MAPK/ERK and PI3K/AKT pathways. These pathways significantly impact cell survival and proliferation. Pan Trk often interacts with proteins like SHC and GRB2 to transmit signals downstream efficiently. These interactions are important for linking the surface receptor activation to the intracellular responses that govern cell growth and survival.

Dysregulation or mutations in Pan Trk pathways contribute to neurodegenerative diseases and cancers. For example in neuroblastoma altered Pan Trk expression can modify cell survival signals resulting in tumor growth. These receptors also relate to Alzheimer's disease where Pan Trk dysfunction can affect neuronal resilience and memory pathways. In these disorders Pan Trk associates with various proteins including Tau which can disrupt normal neuronal processes.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Receptor tyrosine kinase involved in the development and the maturation of the central and the peripheral nervous systems through regulation of neuron survival, proliferation, migration, differentiation, and synapse formation and plasticity (By similarity). Receptor for BDNF/brain-derived neurotrophic factor and NTF4/neurotrophin-4. Alternatively can also bind NTF3/neurotrophin-3 which is less efficient in activating the receptor but regulates neuron survival through NTRK2 (PubMed : 15494731, PubMed : 7574684). Upon ligand-binding, undergoes homodimerization, autophosphorylation and activation (PubMed : 15494731). Recruits, phosphorylates and/or activates several downstream effectors including SHC1, FRS2, SH2B1, SH2B2 and PLCG1 that regulate distinct overlapping signaling cascades. Through SHC1, FRS2, SH2B1, SH2B2 activates the GRB2-Ras-MAPK cascade that regulates for instance neuronal differentiation including neurite outgrowth. Through the same effectors controls the Ras-PI3 kinase-AKT1 signaling cascade that mainly regulates growth and survival. Through PLCG1 and the downstream protein kinase C-regulated pathways controls synaptic plasticity. Thereby, plays a role in learning and memory by regulating both short term synaptic function and long-term potentiation. PLCG1 also leads to NF-Kappa-B activation and the transcription of genes involved in cell survival. Hence, it is able to suppress anoikis, the apoptosis resulting from loss of cell-matrix interactions. May also play a role in neutrophin-dependent calcium signaling in glial cells and mediate communication between neurons and glia.

See full target information NTRK2

style

left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Clinical and translational medicine 15:e70461 PubMed40887856

2025

Dissecting cross-lineage tumourigenesis under p53 inactivation through single-cell multi-omics and spatial transcriptomics.

Applications

Unspecified application

Species

Unspecified reactive species

Xinru Wang,Yuqing Mei,Xueyi Wang,Hanyu Wu,Renying Wang,Peijing Zhang,Guodong Zhang,Jiaqi Li,Mengmeng Jiang,Xing Fang,Lifeng Ma,Yuan Liao,Danmei Jia,Haofu Niu,E Weigao,Haide Chen,Lei Yang,Shuang Zhang,Tingyue Zhang,Yincong Zhou,Qi Zhang,He Huang,Hongwei Ouyang,Ming Chen,Tingbo Liang,Jinrong Peng,Jingjing Wang,Guoji Guo,Xiaoping Han

Dentistry journal 12: PubMed39452455

2024

NTRK Gene Expression Analysis in Oral Squamous Cell Carcinoma Mexican Population.

Applications

Unspecified application

Species

Unspecified reactive species

Lilibeth Stephania Escoto-Vasquez,Javier Portilla-Robertson,Josué Orlando Ramírez-Jarquín,Luis Fernando Jacinto-Alemán,Alejandro Alonso-Moctezuma,Carla Monserrat Ramírez-Martínez,Osmar Alejandro Chanes-Cuevas,Fabiola Salgado-Chavarria

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Pan Trk antibody [EPR17341-4]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pan Trk antibody [EPR17341-4] (AB246551)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pan Trk antibody [EPR17341-4] (AB246551)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pan Trk antibody [EPR17341-4] (AB246551)

Immunoprecipitation - Anti-Pan Trk antibody [EPR17341-4] (AB246551)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pan Trk antibody [EPR17341-4] (AB246551)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pan Trk antibody [EPR17341-4] (AB246551)

Western blot - Anti-Pan Trk antibody [EPR17341-4] (AB246551)

Western blot - Anti-Pan Trk antibody [EPR17341-4] (AB246551)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise