Anti-Pan Trk antibody [EPR17341-4] (ab246551)

Rabbit Recombinant Monoclonal TrkB antibody. Suitable for IP, WB, IHC-P and reacts with Human, Mouse, Rat samples.

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Images

Immunoprecipitation - Anti-Pan Trk antibody [EPR17341-4] (AB246551), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IP	WB	IHC-P	ICC/IF	Flow Cyt
Human	Tested	Tested	Tested	Not recommended	Not recommended
Mouse	Expected	Tested	Tested	Not recommended	Not recommended
Rat	Expected	Tested	Tested	Not recommended	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/30	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/1000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/1000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info 1/1000	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/500	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/500	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info 1/500	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Mouse, Rat	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Mouse, Rat	Dilution info -	Notes -

Target data

See full target information NTRK2

Function

The protein expressed by the NTRK2 gene functions as a receptor for BDNF/brain-derived neurotrophic factor and NTF4/neurotrophin-4, and can also bind to NTF3/neurotrophin-3, although this interaction is less efficient. Upon binding with its ligands, the receptor undergoes homodimerization, autophosphorylation, and activation, enabling it to recruit, phosphorylate, and/or activate several downstream effectors like SHC1, FRS2, SH2B1, SH2B2, and PLCG1. These effectors regulate distinct but overlapping signaling cascades. Through SHC1, FRS2, SH2B1, and SH2B2, NTRK2 activates the GRB2-Ras-MAPK cascade, which regulates neuronal differentiation including neurite outgrowth, as well as the Ras-PI3 kinase-AKT1 signaling cascade, which is primarily involved in growth and survival. The PLCG1 pathway, which influences synaptic plasticity, plays a role in learning and memory by affecting both short-term synaptic function and long-term potentiation. Additionally, PLCG1 activation leads to NF-Kappa-B activation, promoting the transcription of genes related to cell survival, thereby suppressing anoikis. NTRK2 may also be involved in neurotrophin-dependent calcium signaling in glial cells and in mediating communication between neurons and glial cells. This supplementary information is collated from multiple sources and compiled automatically.

Alternative names

TRKB, NTRK2, BDNF/NT-3 growth factors receptor, GP145-TrkB, Neurotrophic tyrosine kinase receptor type 2, TrkB tyrosine kinase, Tropomyosin-related kinase B, Trk-B

Recommended products

Rabbit Recombinant Monoclonal TrkB antibody. Suitable for IP, WB, IHC-P and reacts with Human, Mouse, Rat samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR17341-4
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Pan Trk also known as Pan-tropomyosin receptor kinases are receptors that belong to the neurotrophin receptor family including TrkA TrkB and TrkC. They typically express in neural tissues and play a role in neuronal development and function. The mass of Pan Trk can vary as it encompasses several related proteins rather than a single unit. The variant forms are present in both the central and peripheral nervous systems. Researchers often use tags like Pan Trk IHc 555 Pan and Alexa Pan to identify different forms and monitor their expression patterns.

Biological function summary

Pan Trk receptors play a vital role in the survival development and function of neurons by mediating the action of neurotrophins. They participate as part of a receptor complex activated by ligand binding which triggers autophosphorylation. These receptors modulate various processes like neuronal survival and differentiation. Pan Trk signals generally initiate upon binding neurotrophins leading to multiple intracellular events.

Pathways

Pan Trk receptors are central players in signaling pathways such as the MAPK/ERK and PI3K/AKT pathways. These pathways significantly impact cell survival and proliferation. Pan Trk often interacts with proteins like SHC and GRB2 to transmit signals downstream efficiently. These interactions are important for linking the surface receptor activation to the intracellular responses that govern cell growth and survival.

Associated diseases and disorders

Dysregulation or mutations in Pan Trk pathways contribute to neurodegenerative diseases and cancers. For example in neuroblastoma altered Pan Trk expression can modify cell survival signals resulting in tumor growth. These receptors also relate to Alzheimer's disease where Pan Trk dysfunction can affect neuronal resilience and memory pathways. In these disorders Pan Trk associates with various proteins including Tau which can disrupt normal neuronal processes.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

Immunoprecipitation - Anti-Pan Trk antibody [EPR17341-4] (ab246551)
Pan Trk was immunoprecipitated from 0.35 mg of human fetal brain lysate with ab246551 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab246551 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10,000 dilution.
Lane 1: Human fetal brain lysate 10 μg (Input).
Lane 2: ab246551 IP in human fetal brain lysate (+).
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab246551 in human fetal brain lysate (-).
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds.
The 30kDa band is an intracellular fragment TrkB-ICD, and the 140KDa observed MW is due to the glycosylation modification (PMID: 24860020).
All lanes: Immunoprecipitation - Anti-Pan Trk antibody [EPR17341-4] (ab246551)
Observed band size: 140 kDa, 30 kDa
Western blot - Anti-Pan Trk antibody [EPR17341-4] (ab246551)
Exposure time : Lanes 1 and 2: 3 minutes; Lane 3: 3 seconds.
Blocking and dilution buffer: 5% NFDM/TBST.
The 30kDa band is an intracellular fragment TrkB-ICD, and the 140KDa observed MW is due to the glycosylation modification (PMID: 24860020).
All lanes: Western blot - Anti-Pan Trk antibody [EPR17341-4] (ab246551) at 1/1000 dilution
Lane 1: Rat brain lysate at 20 µg
Lane 2: Human hippocampus lysate at 20 µg
Lane 3: Mouse brain lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 92 kDa
Observed band size: 140 kDa, 30 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pan Trk antibody [EPR17341-4] (ab246551)
Immunohistochemical analysis of paraffin-embedded human cerebral cortex tissue labeling Pan Trk with ab246551 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic staining in neuron of human cerebral cortex (PMID: 7936202) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pan Trk antibody [EPR17341-4] (ab246551)
Immunohistochemical analysis of paraffin-embedded mouse cerebral cortex tissue labeling Pan Trk with ab246551 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic staining in neuron of mouse cerebral cortex (PMID: 2171894) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pan Trk antibody [EPR17341-4] (ab246551)
Immunohistochemical analysis of paraffin-embedded rat cerebral cortex tissue labeling Pan Trk with ab246551 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic staining in neuron of rat cerebral cortex is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pan Trk antibody [EPR17341-4] (ab246551)
Immunohistochemical analysis of paraffin-embedded human liver tissue labeling Pan Trk with ab246551 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Negative staining is observed in normal human liver. Counterstained with hematoxylin.
Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
Western blot - Anti-Pan Trk antibody [EPR17341-4] (ab246551)
Blocking and dilution buffer: 5% NFDM/TBST.
The 30kDa band is an intracellular fragment TrkB-ICD, and the 140KDa observed MW is due to the glycosylation modification (PMID: 24860020).
Negative control: human fetal heart, human fetal kidney, human fetal spleen.
All lanes: Western blot - Anti-Pan Trk antibody [EPR17341-4] (ab246551) at 1/1000 dilution
Lane 1: Human fetal brain lysate at 10 µg
Lane 2: Human fetal heart lysate at 10 µg
Lane 3: Human fetal kidney lysate at 10 µg
Lane 4: Human fetal spleen lysate at 10 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 92 kDa
Observed band size: 140 kDa, 30 kDa
Exposure time: 3min
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pan Trk antibody [EPR17341-4] (ab246551)
Immunohistochemical analysis of paraffin-embedded Human astrocytoma labelling Pan Trk with ab246551 at 1/500 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP polymer) ready to use (Goat Anti-Rabbit IgG H&L (HRP polymer) ab214880).

Positive staining on human astrocytoma is observed. Counter stained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a Goat Anti-Rabbit IgG H&L (HRP polymer) ready to use. Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).