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Proteins and peptidesAnti-Ly6g antibody [1A8] - mouse IgG2c (Chimeric)
Low endotoxin, Azide free.
Our first-to-market chimera with mouse IgG2c backbone, this functional antibody specifically depletes neutrophils in vivo for up to 72h.
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Rabbit anti-Pan Trk antibody EPR17341 ab181560 is a rabbit monoclonal antibody that is used in Pan Trk western blotting, IHC and immunofluorescence. Suitable for human, mouse and rat samples.
Recombinant format for high batch-to-batch consistency and reproducible results
Specificity and sensitivity confirmed in IHC with multi-tissue microarray validation.
Anti-Pan Trk antibody ab181560 is cited in over 30 publications
IgG
Rabbit
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
WB | ICC/IF | IHC-P | |
---|---|---|---|
Human | Tested | Expected | Tested |
Mouse | Tested | Tested | Tested |
Rat | Tested | Expected | Tested |
Chicken | Predicted | Predicted | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/10000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/10000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/10000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Chicken | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/250 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Chicken | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/500 | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/500 | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species Human | Dilution info 1/500 | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Chicken | Dilution info - | Notes - |
Receptor tyrosine kinase involved in the development and the maturation of the central and the peripheral nervous systems through regulation of neuron survival, proliferation, migration, differentiation, and synapse formation and plasticity (By similarity). Receptor for BDNF/brain-derived neurotrophic factor and NTF4/neurotrophin-4. Alternatively can also bind NTF3/neurotrophin-3 which is less efficient in activating the receptor but regulates neuron survival through NTRK2 (PubMed:7574684, PubMed:15494731). Upon ligand-binding, undergoes homodimerization, autophosphorylation and activation (PubMed:15494731). Recruits, phosphorylates and/or activates several downstream effectors including SHC1, FRS2, SH2B1, SH2B2 and PLCG1 that regulate distinct overlapping signaling cascades. Through SHC1, FRS2, SH2B1, SH2B2 activates the GRB2-Ras-MAPK cascade that regulates for instance neuronal differentiation including neurite outgrowth. Through the same effectors controls the Ras-PI3 kinase-AKT1 signaling cascade that mainly regulates growth and survival. Through PLCG1 and the downstream protein kinase C-regulated pathways controls synaptic plasticity. Thereby, plays a role in learning and memory by regulating both short term synaptic function and long-term potentiation. PLCG1 also leads to NF-Kappa-B activation and the transcription of genes involved in cell survival. Hence, it is able to suppress anoikis, the apoptosis resulting from loss of cell-matrix interactions. May also play a role in neutrophin-dependent calcium signaling in glial cells and mediate communication between neurons and glia.
BDNF/NT-3 growth factors receptor, GP145-TrkB, Neurotrophic tyrosine kinase receptor type 2, TrkB tyrosine kinase, Tropomyosin-related kinase B, Trk-B, NTRK2, TRKB
Rabbit anti-Pan Trk antibody EPR17341 ab181560 is a rabbit monoclonal antibody that is used in Pan Trk western blotting, IHC and immunofluorescence. Suitable for human, mouse and rat samples.
Recombinant format for high batch-to-batch consistency and reproducible results
Specificity and sensitivity confirmed in IHC with multi-tissue microarray validation.
Anti-Pan Trk antibody ab181560 is cited in over 30 publications
BDNF/NT-3 growth factors receptor, GP145-TrkB, Neurotrophic tyrosine kinase receptor type 2, TrkB tyrosine kinase, Tropomyosin-related kinase B, Trk-B, NTRK2, TRKB
IgG
Rabbit
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
EPR17341
Affinity purification Protein A
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
This is the Research Use Only (RUO) antibody of the clone that has been used in the in vitro diagnostic VENTANA pan-TRK (EPR17341) assay (an immunohistochemistry assay).
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
The Trk receptors bind with neurotrophins such as nerve growth factor (NGF) brain-derived neurotrophic factor (BDNF) and neurotrophin-3 (NT-3) to initiate signaling that supports neuronal survival and growth. These receptors function as part of a larger receptor-ligand complex. Upon neurotrophin binding the Trk receptors undergo dimerization and transphosphorylation triggering downstream signaling cascades. This activation modulates cellular processes including synaptic strength enhancement and neuronal network formation.
Pan Trk also known as tropomyosin receptor kinase (Trk) includes three different receptors: TrkA TrkB and TrkC. These belong to the neurotrophic tyrosine receptor kinase (NTRK) family each with a mass of roughly 140 kDa. They are mainly expressed in neuronal tissues but can also appear in non-neuronal tissues. The pan Trk term refers to antibodies that detect all three receptors simultaneously. These receptors play an important role in the cellular response to neurotrophins which are essential for differentiation survival and plasticity of neurons.
The Trk receptors activate important cellular signaling pathways such as the PI3K/AKT and MAPK/ERK pathways. These pathways regulate cell survival proliferation and differentiation. The Trk receptors interact with proteins like Shc PLC? and Grb2 which facilitate signal transduction to multiple downstream effectors. This integration into signaling networks allows cross-talk with other signaling molecules and pathways orchestrating complex biological functions.
Pan Trk expression links to conditions like neurodegenerative diseases and certain cancers. Overexpression or mutation of Trk receptors can lead to oncogenesis particularly in tumor types like gliomas and neuroblastomas. Moreover abnormal Trk signaling has associations with Alzheimer's disease where dysregulated nerve growth factor signaling contributes to neurodegeneration. Targeted therapies and pan Trk inhibitors offer potential treatment strategies for these diseases by modulating receptor activity and correcting signaling pathway imbalances.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neural mix culture cells labelling Pan Trk with ab181560 at 1:100 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1:1000 dilution (Green). Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1:200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized Neuro-2a (Mouse neuroblastoma cells) cells labeling Pan Trk with ab181560 at 1/250 dilution. Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/400 dilution was used as the secondary antibody (green). Confocal image showing cytoplasmic staining on Neuro-2a cells is shown. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (Tubulin mouse mAb) at 1/500 and ab150120 (Alexa Fluor® 594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows;
1. ab181560 at 1/250 dilution followed by ab150120 (Goat anti mouse IgG (Alexa Fluor® 594)) at 1/500 dilution.
2. ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by ab150077 (Goat anti rabbit IgG (Alexa Fluor® 488)) at 1/400 dilution.
Immunohistochemical analysis of paraffin-embedded Human astrocytoma tissue labeling Pan Trk with ab181560 at 1/500 dilution, followed by Anti-Rabbit HRP (ab97051) at 1/500 dilution. Astrocytoma cells show strong cytoplasmic staining. Counter stained with Hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0
Negative control: Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG.
TrkB is abundantly expressed in the central and peripheral nervous systems,
human fetal heart and human fetal spleen are used as negative controls.
The 30KDa band is an intracellular fragment TrkB-ICD, and the 140KDa observed MW which is higher than the predicted one is due to the glycosylation modification.
Blocking/dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-Pan Trk antibody [EPR17341] (AB181560) at 1/10000 dilution
Lane 1: Human fetal brain lysates at 20 µg
Lane 2: Human fetal heart lysates at 20 µg
Lane 3: Human fetal spleen lysates at 20 µg
All lanes: Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 92 kDa
Different batches of ab181560 were tested on human brain lysate at 1.5 µg/ml. 15 µg of lysate was loaded in each lane. Bands observed at 30,140 kDa.
All lanes: Western blot - Anti-Pan Trk antibody [EPR17341] (AB181560)
Predicted band size: 92 kDa
Immunohistochemical analysis of paraffin-embedded Human cerebral cortex tissue labeling Pan Trk with ab181560 at 1/500 dilution, followed by Anti-Rabbit HRP (ab97051) at 1/500 dilution. Cytoplasmic staining is observed on neurons of human cerebral cortex. Counter stained with Hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.
Negative control: Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG.
TrkB is abundantly expressed in the central and peripheral nervous system. The 30KDa band is an intracellular fragment TrkB-ICD, and the 140KDa observed MW which is higher than the predicted one is due to the glycosylation modification.
Blocking/dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-Pan Trk antibody [EPR17341] (AB181560) at 1/10000 dilution
All lanes: Human cerebellum lysates at 10 µg
All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 48 kDa, 92 kDa
Immunohistochemical analysis of paraffin-embedded Mouse cerebral cortex tissue labeling Pan Trk with ab181560 at 1/500 dilution, followed by Anti-Rabbit HRP (ab97051) at 1/500 dilution. Cytoplasmic staining is observed on neurons of mouse cerebral cortex. Counter stained with Hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.
Negative control: Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG.
Immunohistochemical analysis of paraffin-embedded Rat cerebral cortex tissue labeling Pan Trk with ab181560 at 1/500 dilution, followed by Anti-Rabbit HRP (ab97051) at 1/500 dilution. Cytoplasmic staining is observed on neurons of Rat cerebral cortex. Counter stained with Hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.
Negative control: Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG.
Immunohistochemical analysis of paraffin-embedded Human (Panel 1), Mouse (Panel 2) or Rat (Panel 3) liver tissue labeling Pan Trk with ab181560 at 1/500 dilution, followed by Anti-Rabbit HRP (ab97051) at 1/500 dilution. The staining is negative on normal Human liver. Counter stained with Hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.
Negative control: Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG.
Tissue Microarrays stained for "Anti-Pan Trk antibody [EPR17341]" using "ab181560"in immunohistochemical analysis. This table provides a detailed overview of positive (tick mark) and negative (cross mark) staining per sample type tested. The sections were pre-treated using Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). The sections were incubated with ab181560 at +4°C overnight followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).
Immunohistochemical analysis of formalin fixed paraffin embedded human cerebrum labelling Pan Trk with ab181560 at a concentration of 5 µg/ml. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with a Bond™ Polymer Refine Detection kit. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution 2) for 20 mins. ab181560 anti-Pan Trk antibody [EPR17341] was incubated for 30 mins at room temperature. Sections were counterstained with Hematoxylin. Image inset shows absence of staining in secondary antibody only control.
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-Pan Trk antibody [EPR17341] (AB181560) at 1/10000 dilution
Lane 1: 293T (human embryonic kidney) transfected with an empty vector (vector control) containing a myc-His-tag®, whole cell lysate at 20 µg
Lane 2: 293T transfected with human TrkA expression vector containing a myc-His-tag®, whole cell lysate at 20 µg
Lane 3: 293T transfected with human TrkB expression vector containing a myc-His-tag®, whole cell lysate at 20 µg
Lane 4: 293T transfected with human TrkC expression vector containing a myc-His-tag®, whole cell lysate at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/20000 dilution
Observed band size: 140 kDa
Exposure time: 100s
Tissue Microarrays stained for "Anti-Pan Trk antibody [EPR17341]" using "ab181560"in immunohistochemical analysis. This table provides a detailed overview of positive (tick mark) and negative (cross mark) staining per sample type tested. The sections were pre-treated using Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). The sections were incubated with ab181560 at +4°C overnight followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).
Immunohistochemical analysis of formalin fixed paraffin embedded human cerebrum labelling Pan Trk with ab181560 at a concentration of 3 µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with an OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was conducted for 32 mins at 100°C with ULTRA cell conditioning solution (CC1, pH 8.5). ab181560 anti-Pan Trk antibody [EPR17341] was incubated at 37°C for 16 mins. Sections were counterstained with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.
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