Anti-Pan Trk antibody [EPR17341] - BSA and Azide free

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pan Trk antibody [EPR17341] - BSA and Azide free (AB218577)

This data was developed using the same antibody clone in a different buffer formulation (ab181560).

Immunohistochemical analysis of formalin fixed paraffin embedded human cerebrum labelling Pan Trk with ab181560 at a concentration of 5 µg/ml. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with a Bond™ Polymer Refine Detection kit. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution 2) for 20 mins. ab181560 anti-Pan Trk antibody [EPR17341] was incubated for 30 mins at room temperature. Sections were counterstained with Hematoxylin. Image inset shows absence of staining in secondary antibody only control.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pan Trk antibody [EPR17341] - BSA and Azide free (AB218577)

Immunohistochemical analysis of paraffin-embedded Human astrocytoma tissue labeling Pan Trk with ab181560 at 1/500 dilution followed by Anti-Rabbit HRP (ab97051) at 1/500 dilution. Astrocytoma cells show strong cytoplasmic staining. Counter stained with Hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer pH 9.0

Negative control : Using PBS instead of primary ab secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181560).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pan Trk antibody [EPR17341] - BSA and Azide free (AB218577)

Immunohistochemical analysis of paraffin-embedded Human cerebral cortex tissue labeling Pan Trk with ab181560 at 1/500 dilution, followed by Anti-Rabbit HRP (ab97051) at 1/500 dilution. Cytoplasmic staining is observed on neurons of human cerebral cortex. Counter stained with Hematoxylin.

Negative control : Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181560).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pan Trk antibody [EPR17341] - BSA and Azide free (AB218577)

Tissue Microarrays stained for "Anti-Pan Trk antibody [EPR17341]" using "ab181560"in immunohistochemical analysis. This table provides a detailed overview of positive (tick mark) and negative (cross mark) staining per sample type tested. The sections were pre-treated using Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). The sections were incubated with ab181560 at +4°C overnight followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pan Trk antibody [EPR17341] - BSA and Azide free (AB218577)

This data was developed using the same antibody clone in a different buffer formulation (ab181560).

Immunohistochemical analysis of formalin fixed paraffin embedded human cerebrum labelling Pan Trk with ab181560 at a concentration of 3 µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with an OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was conducted for 32 mins at 100°C with ULTRA cell conditioning solution (CC1, pH 8.5). ab181560 anti-Pan Trk antibody [EPR17341] was incubated at 37°C for 16 mins. Sections were counterstained with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pan Trk antibody [EPR17341] - BSA and Azide free (AB218577)

Immunohistochemical analysis of paraffin-embedded Mouse cerebral cortex tissue labeling Pan Trk with ab181560 at 1/500 dilution, followed by Anti-Rabbit HRP (ab97051) at 1/500 dilution. Cytoplasmic staining is observed on neurons of mouse cerebral cortex. Counter stained with Hematoxylin.
Negative control : Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181560).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Pan Trk antibody [EPR17341] - BSA and Azide free (AB218577)

Immunofluorescent analysis of 4% paraformaldehyde-fixed 0.1% tritonX-100 permeabilized Neuro-2a (Mouse neuroblastoma cells) cells labeling Pan Trk with ab181560 at 1/250 dilution. Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) at 1/400 dilution was used as the secondary antibody (green). Confocal image showing cytoplasmic staining on Neuro-2a cells is shown. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (Tubulin mouse mAb) at 1/500 and ab150120 (Alexa Fluor^® 594 Goat anti-Mouse secondary) at 1/500 dilution (red).

The negative controls are as follows;
1. ab181560 at 1/250 dilution followed by ab150120 (Goat anti mouse IgG (Alexa Fluor^® 594)) at 1/500 dilution.
2. ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by ab150077 (Goat anti rabbit IgG (Alexa Fluor^® 488)) at 1/400 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181560).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pan Trk antibody [EPR17341] - BSA and Azide free (AB218577)

Immunohistochemical analysis of paraffin-embedded Rat cerebral cortex tissue labeling Pan Trk with ab181560 at 1/500 dilution, followed by Anti-Rabbit HRP (ab97051) at 1/500 dilution. Cytoplasmic staining is observed on neurons of Rat cerebral cortex. Counter stained with Hematoxylin.
Negative control : Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181560).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pan Trk antibody [EPR17341] - BSA and Azide free (AB218577)

Tissue Microarrays stained for "Anti-Pan Trk antibody [EPR17341]" using "ab181560"in immunohistochemical analysis. This table provides a detailed overview of positive (tick mark) and negative (cross mark) staining per sample type tested. The sections were pre-treated using Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). The sections were incubated with ab181560 at +4°C overnight followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Pan Trk antibody [EPR17341] - BSA and Azide free (AB218577)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed 0.1% TritonX-100 permeabilized mouse primary neural mix culture cells labelling Pan Trk with ab181560 at 1 : 100 dilution followed by ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary antibody at 1 : 1000 dilution (Green). Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1 : 200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181560).

• WB

Lab

Western blot - Anti-Pan Trk antibody [EPR17341] - BSA and Azide free (AB218577)

This data was developed using ab181560, the same antibody clone in a different buffer formulation. Different batches of ab181560 were tested on human brain lysate at 1.5 µg/ml. 15 µg of lysate was loaded in each lane. Bands observed at 30,140 kDa.

All lanes:

Western blot - Anti-Pan Trk antibody [EPR17341] (<a href='/en-us/products/primary-antibodies/pan-trk-antibody-epr17341-ab181560'>ab181560</a>)

Predicted band size: 92 kDa

false

• WB

Lab

Western blot - Anti-Pan Trk antibody [EPR17341] - BSA and Azide free (AB218577)

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Pan Trk antibody [EPR17341] (<a href='/en-us/products/primary-antibodies/pan-trk-antibody-epr17341-ab181560'>ab181560</a>) at 1/10000 dilution

Lane 1:

293T (human embryonic kidney) transfected with an empty vector (vector control) containing a myc-His-tag®, whole cell lysate at 20 µg

Lane 2:

293T transfected with human TrkA expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

Lane 3:

293T transfected with human TrkB expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

Lane 4:

293T transfected with human TrkC expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 140 kDa

false

Exposure time: 100s