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Rabbit Recombinant Monoclonal TrkC antibody. Suitable for IP, WB and reacts with Mouse, Rat, Human samples.

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Images

Western blot - Anti-Pan Trk antibody [EPR18413] (AB189903), expandable thumbnail
  • Western blot - Anti-Pan Trk antibody [EPR18413] (AB189903), expandable thumbnail
  • Immunoprecipitation - Anti-Pan Trk antibody [EPR18413] (AB189903), expandable thumbnail
  • Western blot - Anti-Pan Trk antibody [EPR18413] (AB189903), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWB
Human
Expected
Tested
Mouse
Tested
Tested
Rat
Expected
Tested

Tested
Tested

Species

Mouse

Dilution info

1/40

Notes

-

Expected
Expected

Species

Rat, Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/1000

Notes

-

Species

Rat

Dilution info

1/1000

Notes

-

Species

Human

Dilution info

1/1000

Notes

-

Associated Products

Select an associated product type

3 products for Alternative Product

Target data

Function

Receptor tyrosine kinase involved in nervous system and probably heart development. Upon binding of its ligand NTF3/neurotrophin-3, NTRK3 autophosphorylates and activates different signaling pathways, including the phosphatidylinositol 3-kinase/AKT and the MAPK pathways, that control cell survival and differentiation.

Alternative names

NT-3 growth factor receptor, GP145-TrkC, Neurotrophic tyrosine kinase receptor type 3, TrkC tyrosine kinase, Trk-C, NTRK3, TRKC

Recommended products

Rabbit Recombinant Monoclonal TrkC antibody. Suitable for IP, WB and reacts with Mouse, Rat, Human samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR18413

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Pan Trk also known as Pan-tropomyosin receptor kinases are receptors that belong to the neurotrophin receptor family including TrkA TrkB and TrkC. They typically express in neural tissues and play a role in neuronal development and function. The mass of Pan Trk can vary as it encompasses several related proteins rather than a single unit. The variant forms are present in both the central and peripheral nervous systems. Researchers often use tags like Pan Trk IHc 555 Pan and Alexa Pan to identify different forms and monitor their expression patterns.

Biological function summary

Pan Trk receptors play a vital role in the survival development and function of neurons by mediating the action of neurotrophins. They participate as part of a receptor complex activated by ligand binding which triggers autophosphorylation. These receptors modulate various processes like neuronal survival and differentiation. Pan Trk signals generally initiate upon binding neurotrophins leading to multiple intracellular events.

Pathways

Pan Trk receptors are central players in signaling pathways such as the MAPK/ERK and PI3K/AKT pathways. These pathways significantly impact cell survival and proliferation. Pan Trk often interacts with proteins like SHC and GRB2 to transmit signals downstream efficiently. These interactions are important for linking the surface receptor activation to the intracellular responses that govern cell growth and survival.

Associated diseases and disorders

Dysregulation or mutations in Pan Trk pathways contribute to neurodegenerative diseases and cancers. For example in neuroblastoma altered Pan Trk expression can modify cell survival signals resulting in tumor growth. These receptors also relate to Alzheimer's disease where Pan Trk dysfunction can affect neuronal resilience and memory pathways. In these disorders Pan Trk associates with various proteins including Tau which can disrupt normal neuronal processes.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-Pan Trk antibody [EPR18413] (ab189903), expandable thumbnail

    Western blot - Anti-Pan Trk antibody [EPR18413] (ab189903)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    TrkB is abundantly expressed in the central and peripheral nervous system. The 30KDa band is an intracellular fragment TrkB-ICD, and the 140KDa observed MW which is higher than the predicted one is due to the glycosylation modification.

    All lanes: Western blot - Anti-Pan Trk antibody [EPR18413] (ab189903) at 1/1000 dilution

    Lane 1: Human brain tissue lysate at 20 µg

    Lane 2: SH-SY5Y (human neuroblastoma cell line from bone marrow) whole cell lysate at 20 µg

    Secondary

    Lane 1: Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Lane 2: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 94 kDa

    Observed band size: 140 kDa, 30 kDa

    Exposure time: 3min

  • Western blot - Anti-Pan Trk antibody [EPR18413] (ab189903), expandable thumbnail

    Western blot - Anti-Pan Trk antibody [EPR18413] (ab189903)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    TrkB is abundantly expressed in the central and peripheral nervous system. The 30KDa band is an intracellular fragment TrkB-ICD, and the 140KDa observed MW which is higher than the predicted one is due to the glycosylation modification.

    All lanes: Western blot - Anti-Pan Trk antibody [EPR18413] (ab189903) at 1/1000 dilution

    Lane 1: Mouse brain tissue lysate at 10 µg

    Lane 2: Mouse heart tissue lysate at 10 µg

    Lane 3: Mouse kidney tissue lysate at 10 µg

    Lane 4: Mouse spleen tissue lysate at 10 µg

    Lane 5: Rat brain tissue lysate at 10 µg

    Lane 6: Rat heart tissue lysate at 10 µg

    Lane 7: Rat kidney tissue lysate at 10 µg

    Lane 8: Rat spleen tissue lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 54 kDa, 94 kDa

    Observed band size: 140 kDa, 30 kDa, 72 kDa

    Exposure time: 3min

    This data was developed using ab189903, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    TrkB is abundantly expressed in the central and peripheral nervous system. The 30KDa band is an intracellular fragment TrkB-ICD, and the 140KDa observed MW which is higher than the predicted one is due to the glycosylation modification.

  • Immunoprecipitation - Anti-Pan Trk antibody [EPR18413] (ab189903), expandable thumbnail

    Immunoprecipitation - Anti-Pan Trk antibody [EPR18413] (ab189903)

    TrkC (phospho Y820) was immunoprecipitated from 1 mg of mouse brain whole cell lysate with ab189903 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab189903 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used as secondary antibody at 1/1000 dilution.

    Lane 1: Mouse brain whole cell lysate 10 μg (Input).

    Lane 2: ab189903 IP in mouse brain whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab189903 in mouse brain whole cell lysate.

    Exposure time: 5 seconds.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    All lanes: Immunoprecipitation - Anti-Pan Trk antibody [EPR18413] (ab189903)

    Observed band size: 140 kDa, 30 kDa

  • Western blot - Anti-Pan Trk antibody [EPR18413] (ab189903), expandable thumbnail

    Western blot - Anti-Pan Trk antibody [EPR18413] (ab189903)

    Exposure times: Lane 1-2: 1 minute; Lane 3-6: 30 seconds.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Pan Trk antibody [EPR18413] (ab189903) at 1/5000 dilution

    Lanes 1, 3 and 5: HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate transfected with empty vector (vector control) at 20 µg

    Lane 2: HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate transfected with TrkA with His/Myc-tag at 20 µg

    Lane 4: HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate transfected with TrkB with His/Myc-tag at 20 µg

    Lane 6: HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate transfected with TrkC with His/Myc-tag at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 94 kDa

    This data was developed using ab189903, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times: Lanes 1-2: 1 minute; Laned 3-6: 30 seconds.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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