Anti-Pan Trk antibody [EPR24009-335]

17 product images

• 

  Western blot - Anti-Pan Trk antibody [EPR24009-335] (ab267830)

  Blocking and diluting buffer and concentration: 5% NFDM/TBST

  All lanes: Western blot - Anti-Pan Trk antibody [EPR24009-335] (ab267830) at 1/1000 dilution

  Lane 1: 293T (human embryonic kidney) transfected with an empty vector (vector control) containi a myc-His-tag®, whole cell lysate 20

  Lane 2: 293T transfected with human TrkB expression vector containi a myc-His-tag®, whole cell lysate 20

  Lane 3: 293T transfected with human TrkC expression vector containi a myc-His-tag®, whole cell lysate 20

  Lane 4: 293T transfected with human TrkA expression vector containi a myc-His-tag®, whole cell lysate 20

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

  Performed under reducing conditions.

  Observed band size: 140 kDa

  Exposure time: 3min

• 

  Western blot - Anti-Pan Trk antibody [EPR24009-335] (ab267830)

  Blocking and diluting buffer and concentration: 5% NFDM/TBST

  All lanes: Western blot - Anti-Pan Trk antibody [EPR24009-335] (ab267830) at 1/1000 dilution

  Lane 1: Human brain tissue lysate

  Lane 2: Human heart tissue lysate

  Lane 3: Human kidney tissue lysate

  Lane 4: Human spleen tissue lysate

  Secondary

  All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

  Performed under reducing conditions.

  Observed band size: 140 kDa

  Exposure time: 15s

• 

  Flow Cytometry (Intracellular) - Anti-Pan Trk antibody [EPR24009-335] (ab267830)

  Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized SD Rat primary neuron cells labelling Pan Trk with ab267830 at 1/500 dilution (0.1ug)/ Right (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti-Rabbit IgG Fc (Alexa Fluor® 488, Goat Anti-Rabbit IgG Fc (Alexa Fluor® 488) preadsorbed ab150097) at 1/2000 dilution was used as the secondary antibody.

• 

  Flow Cytometry (Intracellular) - Anti-Pan Trk antibody [EPR24009-335] (ab267830)

  Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized ICR Mouse primary neuron cells labelling Pan Trk with ab267830 at 1/500 dilution (0.1ug)/ Right (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti-Rabbit IgG Fc (Alexa Fluor® 488, Goat Anti-Rabbit IgG Fc (Alexa Fluor® 488) preadsorbed ab150097) at 1/2000 dilution was used as the secondary antibody.

• 

  Western blot - Anti-Pan Trk antibody [EPR24009-335] (ab267830)

  Blocking and diluting buffer and concentration: 5% NFDM/TBST

  All lanes: Western blot - Anti-Pan Trk antibody [EPR24009-335] (ab267830) at 1/1000 dilution

  Lane 1: Rat brain tissue lysate

  Lane 2: Rat heart tissue lysate

  Lane 3: Rat kidney tissue lysate

  Lane 4: Rat spleen tissue lysate

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

  Performed under reducing conditions.

  Observed band size: 140 kDa

  Exposure time: 59s

• 

  Immunoprecipitation - Anti-Pan Trk antibody [EPR24009-335] (ab267830)

  Pan Trk was immunoprecipitated from 0.35 mg Mouse brain tissue lysate 10 ug with ab267830 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab267830 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

  Lane 1: Mouse brain tissue lysate 10 ug

  Lane 2: abAB267830 IP in Mouse brain tissue lysate

  Lane 3:Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab267830 in mouse brain tissue lysate

  Blocking and dilution buffer and concentration: 5% NFDM/TBST.

  Exposure time: 8 seconds

  All lanes: Immunoprecipitation - Anti-Pan Trk antibody [EPR24009-335] (ab267830) at 1/1000 dilution

  All lanes: Mouse brain tissue lysate at 10 µg

  Secondary

  All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

  Exposure time: 8s

• 

  Immunoprecipitation - Anti-Pan Trk antibody [EPR24009-335] (ab267830)

  Pan Trk was immunoprecipitated from 0.35 mg Rat brain tissue lysate 10 ug with ab267830 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab267830 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

  Lane 1: Rat brain tissue lysate 10 ug

  Lane 2: abAB267830 IP in Rat brain tissue lysate

  Lane 3:Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab267830 in rat brain tissue lysate

  Blocking and dilution buffer and concentration: 5% NFDM/TBST.

  Exposure time: 8 seconds

  All lanes: Immunoprecipitation - Anti-Pan Trk antibody [EPR24009-335] (ab267830) at 1/1000 dilution

  All lanes: Rat brain tissue lysate at 10 µg

  Secondary

  All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

  Exposure time: 8s

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pan Trk antibody [EPR24009-335] (ab267830)

  Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Pan Trk with ab267830 at 1/200 (2.74 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on human cerebrum (PMID: 26171003).The section was incubated with ab267830 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
  
  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
  
  Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pan Trk antibody [EPR24009-335] (ab267830)

  Immunohistochemical analysis of paraffin-embedded Human glioblastoma tissue labeling Pan Trk with ab267830 at 1/200 (2.74 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on human glioblastoma (PMID: 26171003).The section was incubated with ab267830 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
  
  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
  
  Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pan Trk antibody [EPR24009-335] (ab267830)

  Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Pan Trk with ab267830 at 1/200 (2.74 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on mouse cerebrum.The section was incubated with ab267830 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
  
  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
  
  Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pan Trk antibody [EPR24009-335] (ab267830)

  Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling Pan Trk with ab267830 at 1/200 (2.74 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on rat cerebrum.The section was incubated with ab267830 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
  
  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
  
  Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pan Trk antibody [EPR24009-335] (ab267830)

  Immunohistochemical analysis of paraffin-embedded Panel A HEK-293T ( tissue labeling Pan Trk with ab267830 at 1/200 (2.74 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on (A) HEK-293T transfected with TrkC expression vector, (B) HEK-293T transfected with a TrkA expression vector, and (C) HEK-293T transfected with TrkB expression vector. Almost no staining on (D) HEK-293T transfected with empty vector. The section was incubated with ab267830 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
  
  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
  
  Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pan Trk antibody [EPR24009-335] (ab267830)

  Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Pan Trk with ab267830 at 1/200 (2.74 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Negative control: almost no staining on human liver.The section was incubated with ab267830 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
  
  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
  
  Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pan Trk antibody [EPR24009-335] (ab267830)

  Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Pan Trk with ab267830 at 1/200 (2.74 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Negative control: almost no staining on human kidney.The section was incubated with ab267830 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
  
  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
  
  Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pan Trk antibody [EPR24009-335] (ab267830)

  Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Pan Trk with ab267830 at 1/200 (2.74 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Negative control: almost no staining on mouse liver.The section was incubated with ab267830 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
  
  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
  
  Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pan Trk antibody [EPR24009-335] (ab267830)

  Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling Pan Trk with ab267830 at 1/200 (2.74 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Negative control: almost no staining on rat liver.The section was incubated with ab267830 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
  
  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
  
  Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• 

  Western blot - Anti-Pan Trk antibody [EPR24009-335] (ab267830)

  Blocking and diluting buffer and concentration: 5% NFDM/TBST

  All lanes: Western blot - Anti-Pan Trk antibody [EPR24009-335] (ab267830) at 1/1000 dilution

  Lane 1: Mouse brain tissue lysate

  Lane 2: Mouse heart tissue lysate

  Lane 3: Mouse kidney tissue lysate

  Lane 4: Mouse spleen tissue lysate

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

  Performed under reducing conditions.

  Observed band size: 140 kDa

  Exposure time: 37s