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AB222810

Anti-Pan2 antibody

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(1 Publication)

Rabbit Polyclonal PAN2 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human PAN2 aa 600-950.

View Alternative Names

KIAA0710, USP52, PAN2, PAN2-PAN3 deadenylation complex catalytic subunit PAN2, Inactive ubiquitin carboxyl-terminal hydrolase 52, PAB1P-dependent poly(A)-specific ribonuclease, Poly(A)-nuclease deadenylation complex subunit 2, PAN deadenylation complex subunit 2

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-Pan2 antibody (AB222810)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Pan2 antibody (AB222810)

HepG2 (human liver hepatocellular carcinoma cell line) cells stained for Pan2 (green) using ab222810 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pan2 antibody (AB222810)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pan2 antibody (AB222810)

Paraffin-embedded human liver tissue stained for Pan2 using ab222810 at 1/100 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human PAN2 aa 600-950. The exact immunogen used to generate this antibody is proprietary information.

Q504Q3

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%.
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Pan2 is a protein also known as PAN2 poly(A) specific ribonuclease or PAN2-1. It functions as an essential component of the Pan2-Pan3 deadenylation complex responsible for shortening poly(A) tails of mRNAs. This action plays an important role in mRNA turnover and regulation of gene expression. Pan2 has a mass of approximately 162 kDa. Scientists have observed its expression in various tissues with notable presence in the cytoplasm where it interacts with other elements of the mRNA decay machinery.
Biological function summary

The targeted actions of Pan2 facilitate the regulation of mRNA stability which influences protein synthesis rates. Pan2 is part of the Pan2-Pan3 complex where it collaborates with Pan3 to catalyze the initial steps of deadenylation. This process is critical for normal cellular function affecting mRNA lifespan and its translation into protein. In this complex Pan2 provides the catalytic activity while Pan3 enhances its function and anchors the complex to the mRNA substrate.

Pathways

The function of Pan2 in mRNA degradation places it in the mRNA surveillance pathway and the general mRNA decay pathway. These pathways ensure proper regulation and quality control of mRNA within the cell. Pan2 works closely with the CCR4-NOT complex a significant player in mRNA deadenylation. These interactions allow for efficient control over gene expression by modulating the stability and availability of mRNA for translation.

The dysregulation of Pan2 has been implicated in cancer and neurodegenerative diseases. Abnormal mRNA decay processes related to malfunctioning Pan2 can lead to uncontrolled cell growth and tumorigenesis. Additionally Pan2 malfunction may connect to proteins like NOT1 influential in other disorders for instance neurodegeneration. These associations highlight the protein's potential role in pathologies necessitating continued research for better understanding and treatment strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalytic subunit of the poly(A)-nuclease (PAN) deadenylation complex, one of two cytoplasmic mRNA deadenylases involved in general and miRNA-mediated mRNA turnover. PAN specifically shortens poly(A) tails of RNA and the activity is stimulated by poly(A)-binding protein (PABP). PAN deadenylation is followed by rapid degradation of the shortened mRNA tails by the CCR4-NOT complex. Deadenylated mRNAs are then degraded by two alternative mechanisms, namely exosome-mediated 3'-5' exonucleolytic degradation, or deadenylation-dependent mRNA decaping and subsequent 5'-3' exonucleolytic degradation by XRN1. Also acts as an important regulator of the HIF1A-mediated hypoxic response. Required for HIF1A mRNA stability independent of poly(A) tail length regulation.
See full target information PAN2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of gastrointestinal oncology 15:1870-1879 PubMed39279976

2024

The emerging role of FAM46C as a biomarker and therapeutic target in gastric adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Shelly Luu,Ning Fu,Paul Savage,Karina Pacholczyk,Taylor Zaslavsky,James Conner,Carol J Swallow
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Product promise

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