AB241505

Anti-Pan2 antibody

Name: Anti-Pan2 antibody (ab241505) | Abcam
Brand: Abcam Limited
SKU: AB241505
Price: 525 USD
Availability: InStock

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(3 Publications)

Rabbit Polyclonal PAN2 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human PAN2 aa 1-50.

View Alternative Names

KIAA0710, USP52, PAN2, PAN2-PAN3 deadenylation complex catalytic subunit PAN2, Inactive ubiquitin carboxyl-terminal hydrolase 52, PAB1P-dependent poly(A)-specific ribonuclease, Poly(A)-nuclease deadenylation complex subunit 2, PAN deadenylation complex subunit 2

2 Images

Supplier Data

Immunoprecipitation - Anti-Pan2 antibody (AB241505)

Pan2 was immunoprecipitated from HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.
ab241505 used for IP at 6 μg per reaction. For WB 1 μg/ml.

Lane 1 : ab241505 IP in HEK-293T whole cell lysate.
Lane 2 : Control IgG in HEWK-293T whole cell lysate.

Chemiluminescence detection : 3 minutes.

All lanes:

Immunoprecipitation - Anti-Pan2 antibody (ab241505)

Predicted band size: 135 kDa

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Supplier Data

Western blot - Anti-Pan2 antibody (AB241505)

Prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-Pan2 antibody (ab241505) at 0.1 µg/mL

Lane 1:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Lane 4:

TCMK-1 (Mouse kidney epithelial cell line) whole cell lysate at 50 µg

Lane 5:

NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate at 50 µg

Predicted band size: 135 kDa

false

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human PAN2 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q504Q3

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Antibody was affinity purified using an epitope specific to Pan2 immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Pan2 is a protein also known as PAN2 poly(A) specific ribonuclease or PAN2-1. It functions as an essential component of the Pan2-Pan3 deadenylation complex responsible for shortening poly(A) tails of mRNAs. This action plays an important role in mRNA turnover and regulation of gene expression. Pan2 has a mass of approximately 162 kDa. Scientists have observed its expression in various tissues with notable presence in the cytoplasm where it interacts with other elements of the mRNA decay machinery.

Biological function summary

The targeted actions of Pan2 facilitate the regulation of mRNA stability which influences protein synthesis rates. Pan2 is part of the Pan2-Pan3 complex where it collaborates with Pan3 to catalyze the initial steps of deadenylation. This process is critical for normal cellular function affecting mRNA lifespan and its translation into protein. In this complex Pan2 provides the catalytic activity while Pan3 enhances its function and anchors the complex to the mRNA substrate.

Pathways

The function of Pan2 in mRNA degradation places it in the mRNA surveillance pathway and the general mRNA decay pathway. These pathways ensure proper regulation and quality control of mRNA within the cell. Pan2 works closely with the CCR4-NOT complex a significant player in mRNA deadenylation. These interactions allow for efficient control over gene expression by modulating the stability and availability of mRNA for translation.

The dysregulation of Pan2 has been implicated in cancer and neurodegenerative diseases. Abnormal mRNA decay processes related to malfunctioning Pan2 can lead to uncontrolled cell growth and tumorigenesis. Additionally Pan2 malfunction may connect to proteins like NOT1 influential in other disorders for instance neurodegeneration. These associations highlight the protein's potential role in pathologies necessitating continued research for better understanding and treatment strategies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalytic subunit of the poly(A)-nuclease (PAN) deadenylation complex, one of two cytoplasmic mRNA deadenylases involved in general and miRNA-mediated mRNA turnover. PAN specifically shortens poly(A) tails of RNA and the activity is stimulated by poly(A)-binding protein (PABP). PAN deadenylation is followed by rapid degradation of the shortened mRNA tails by the CCR4-NOT complex. Deadenylated mRNAs are then degraded by two alternative mechanisms, namely exosome-mediated 3'-5' exonucleolytic degradation, or deadenylation-dependent mRNA decaping and subsequent 5'-3' exonucleolytic degradation by XRN1. Also acts as an important regulator of the HIF1A-mediated hypoxic response. Required for HIF1A mRNA stability independent of poly(A) tail length regulation.

See full target information PAN2

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Molecular cell 83:2290-2302.e13 PubMed37295431

2023

Mechanism of ribosome-associated mRNA degradation during tubulin autoregulation.

Applications

Unspecified application

Species

Unspecified reactive species

Markus Höpfler,Eva Absmeier,Sew-Yeu Peak-Chew,Evangelia Vartholomaiou,Lori A Passmore,Ivana Gasic,Ramanujan S Hegde

International journal of molecular sciences 23: PubMed36233342

2022

RAI14 Promotes Melanoma Progression by Regulating the FBXO32/c-MYC Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Xu,Pengfei Shi,Fanwei Xia,Xuan Zhao,Junfan Chen,Rui Geng,Hongjuan Cui,Liqun Yang

Nature communications 11:5362 PubMed33097710

2020

USP52 regulates DNA end resection and chemosensitivity through removing inhibitory ubiquitination from CtIP.

Applications

Unspecified application

Species

Unspecified reactive species

Ming Gao,Guijie Guo,Jinzhou Huang,Jake A Kloeber,Fei Zhao,Min Deng,Xinyi Tu,Wootae Kim,Qin Zhou,Chao Zhang,Ping Yin,Kuntian Luo,Zhenkun Lou

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Pan2 antibody

Immunoprecipitation - Anti-Pan2 antibody (AB241505)

Western blot - Anti-Pan2 antibody (AB241505)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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