AB231119

Anti-Pancreatic alpha amylase antibody

Name: Anti-Pancreatic alpha amylase antibody (ab231119) | Abcam
Brand: Abcam Limited
SKU: AB231119

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(2 Publications)

Rabbit Polyclonal AMYP antibody. Suitable for WB, IHC-P and reacts with Rat samples. Cited in 2 publications.

View Alternative Names

Pancreatic alpha-amylase, PA, Amy2

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pancreatic alpha amylase antibody (AB231119)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pancreatic alpha amylase antibody (AB231119)

Formalin-fixed, paraffin-embedded rat pancreas tissue stained for Pancreatic alpha amylase with ab231119 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Supplier Data

Western blot - Anti-Pancreatic alpha amylase antibody (AB231119)

All lanes:

Western blot - Anti-Pancreatic alpha amylase antibody (ab231119) at 2 µg/mL

All lanes:

Rat pancreas lysate

Predicted band size: 58 kDa

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Supplier Data

Western blot - Anti-Pancreatic alpha amylase antibody (AB231119)

All lanes:

Western blot - Anti-Pancreatic alpha amylase antibody (ab231119) at 2 µg/mL

All lanes:

Rat serum

Predicted band size: 58 kDa

true

Supplier Data

Western blot - Anti-Pancreatic alpha amylase antibody (AB231119)

All lanes:

Western blot - Anti-Pancreatic alpha amylase antibody (ab231119) at 2 µg/mL

All lanes:

Recombinant rat Pancreatic alpha amylase protein

Predicted band size: 58 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat

Applications

IHC-P, WB

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "<p></p>" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Pancreatic alpha amylase also known as AMY2A is an enzyme responsible for catalyzing the hydrolysis of starch into sugars. This enzyme is an important amylase protein with a molecular mass of approximately 56 kDa. It is abundantly expressed in the pancreas of mammals playing a significant role in the digestive process. The product of its activity maltose contributes to carbohydrate digestion allowing for energy production in the body.

Biological function summary

Pancreatic alpha amylase assists in the breakdown of large polysaccharides into smaller maltose units. This enzyme can act independently and does not require a complex to function effectively. It carries out its activity primarily in the small intestine where it continues the digestive process initiated by salivary alpha amylase. This amylase protein ensures that starches consumed in the diet are converted into forms absorbable by the body contributing to overall metabolism.

Pathways

Pancreatic alpha amylase is a critical enzyme in the digestive pathway especially in the carbohydrate digestion and absorption pathway. Its activity complements that of other amylase enzymes including salivary alpha amylase to ensure efficient starch breakdown. Additionally pancreatic alpha amylase works in conjunction with proteins like glucose transporters which facilitate the entry of glucose into cells after starch breakdown.

The activity of pancreatic alpha amylase often links to conditions such as pancreatitis and type 2 diabetes. Elevated levels of this enzyme in the bloodstream can indicate acute pancreatitis highlighting its significance in diagnosing this disorder. In the context of diabetes altered pancreatic amylase activity can affect glucose metabolism. The enzyme's association with glucose-regulating proteins such as insulin makes it a focal point in understanding diabetic pathophysiology.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Amy2

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine (Cambridge, Mass.) 31:253 PubMed40618046

2025

Targeting PFKFB3 to restore glucose metabolism in acute pancreatitis via nanovesicle delivery.

Applications

Unspecified application

Species

Unspecified reactive species

Hai Jiang,Zhipeng Xu,Qi Song,Junjie Tao,Jia Liu,Qiang Wang,Huaisheng Zhang,Heng Zhu,Qiliang Li,Lei Li

International journal of molecular sciences 23: PubMed35743192

2022

Copper-Binding Domain Variation in a Novel Murine Lysyl Oxidase Model Produces Structurally Inferior Aortic Elastic Fibers Whose Failure Is Modified by Age, Sex, and Blood Pressure.

Applications

Unspecified application

Species

Unspecified reactive species

Kit Man Tsang,Russell H Knutsen,Charles J Billington,Eric Lindberg,Heiko Steenbock,Yi-Ping Fu,Amanda Wardlaw-Pickett,Delong Liu,Daniela Malide,Zu-Xi Yu,Christopher K E Bleck,Jürgen Brinckmann,Beth A Kozel

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Pancreatic alpha amylase antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pancreatic alpha amylase antibody (AB231119)

Western blot - Anti-Pancreatic alpha amylase antibody (AB231119)

Western blot - Anti-Pancreatic alpha amylase antibody (AB231119)

Western blot - Anti-Pancreatic alpha amylase antibody (AB231119)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise