JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB308358

Anti-Pancreatic Lipase antibody [HL1310]

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal Pancreatic Lipase/PTL antibody. Carrier free. Suitable for IHC-P, WB and reacts with Mouse, Rat, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human PNLIP.

View Alternative Names

Pancreatic triacylglycerol lipase, PL, PTL, Pancreatic lipase, PNLIP

2 Images
Immunohistochemistry paraffin embedded sections - Anti-Pancreatic Lipase antibody [HL1310] (AB308358)
  • IHC-P

Supplier Data

Immunohistochemistry paraffin embedded sections - Anti-Pancreatic Lipase antibody [HL1310] (AB308358)

Immunohistochemical analysis of rat pancreas tissue labeling Pancreatic Lipase using ab308358 at 1/100 dilution. Antigen retrival was performed using Citrate buffer, pH 6.0, 15 min

Western blot - Anti-Pancreatic Lipase antibody [HL1310] (AB308358)
  • WB

Supplier Data

Western blot - Anti-Pancreatic Lipase antibody [HL1310] (AB308358)

Separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-Pancreatic Lipase antibody [HL1310] (ab308358) at 1/1000 dilution

All lanes:

Human pancreas tissue lysate at 10 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 51.2 kDa

true

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1310

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse, Rat

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human PNLIP. The exact immunogen used to generate this antibody is proprietary information.

P16233

style
left-column

Complementary Products

Assay Kits

AB102524

Lipase Assay Kit (Colorimetric)

Functional Studies - Lipase Assay Kit (Colorimetric) (AB102524)

  • 0
  • 0
Primary Antibodies

AB199132

Anti-Pancreatic alpha amylase antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pancreatic alpha amylase antibody (AB199132)

  • 5
  • 2
Primary Antibodies

AB52625

Anti-Cytokeratin 19 antibody [EP1580Y] - Cytoskeleton Marker

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Multiplex immunohistochemistry - Anti-Cytokeratin 19 antibody [EP1580Y] - Cytoskeleton Marker (AB52625)

  • 5
  • 12
Primary Antibodies

AB187164

Anti-Chymotrypsin antibody [EPR13859]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-Chymotrypsin antibody [EPR13859] (AB187164)

  • 0
  • 0
Primary Antibodies

AB194697

Anti-UGT1A1 antibody

Western blot - Anti-UGT1A1 antibody (AB194697)

  • 4
  • 2
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/100000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/100000", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/100000", "WB-species-notes": "<p></p>" } } }
style
left-column

Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Pancreatic Lipase also known as PTL or Lipase Pancreas is an enzyme with a molecular mass around 3000 daltons. It is important for the digestion of dietary fats and is expressed in the pancreas of several animals including the mouse and rat. The enzyme hydrolyzes triglycerides into monoglycerides and free fatty acids facilitating the absorption of fat-soluble vitamins. The presence of pancreatic lipase in rodent models like 'pancreas in rat' or 'mouse pancreas' aids in studying its mechanical function which is significant for understanding lipid metabolism.
Biological function summary

Pancreatic lipase plays a central role in the digestive system converting dietary fats into absorbable units. Although primarily not part of any complex its activity depends on the presence of bile salts and co-lipase. Co-lipase binds to pancreatic lipase and enhances its efficiency by stabilizing the enzyme-substrate complex in the presence of bile supporting lipid digestion and absorption.

Pathways

The enzyme is an essential component of the lipid digestion and absorption pathways. It operates closely with other lipases like gastric lipase sequentially breaking down fats from different stages of digestion. The pathways involve the secretion of bile from the liver which emulsifies fats enabling pancreatic lipase to act more efficiently. As part of the larger digestion process the enzyme plays a role in maintaining metabolic homeostasis by interlinking with hormones like insulin which further regulates blood glucose levels based on energy intake from fats.

Pancreatic lipase has significant implications in conditions like hyperlipidemia and pancreatitis. Defects or deficiencies in pancreatic lipase activity can result in malabsorption syndromes affecting nutritional status and leading to weight loss and vitamin deficiencies. Chronic pancreatitis an inflammatory disease with progressive exocrine pancreatic insufficiency involves diminished pancreatic lipase function often requiring enzyme supplementation as part of the treatment. The connection of pancreatic lipase to hyperlipidemia relates to its interaction with proteins like lipoprotein lipase which manages lipid levels within the bloodstream.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Plays an important role in fat metabolism. It preferentially splits the esters of long-chain fatty acids at positions 1 and 3, producing mainly 2-monoacylglycerol and free fatty acids, and shows considerably higher activity against insoluble emulsified substrates than against soluble ones.
See full target information PNLIP
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com