Goat Polyclonal Pancreatic Polypeptide antibody. Suitable for WB, ELISA, ICC/IF, IHC-P, Flow Cyt (Intra) and reacts with Human, Mouse samples. Cited in 21 publications. Immunogen corresponding to Synthetic Peptide within Human Pancreatic prohormone aa 50 to C-terminus.
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- iScience 26:1065002023Loss of Fgf9 in mice leads to pancreatic hypoplasia and asplenia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSophie Patzek,Zhe Liu,Sean de la O,Sean Chang,Lauren E Byrnes,Xiuqin Zhang,David M Ornitz,Julie B SneddonPubMed 37096042
- Cell reports 37:1099192021Single-cell analysis of the human pancreas in type 2 diabetes using multi-spectral imaging mass cytometry.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMinghui Wu,Michelle Y Y Lee,Varun Bahl,Daniel Traum,Jonathan Schug,Irina Kusmartseva,Mark A Atkinson,Guanjie Fan,Klaus H KaestnerPubMed 34731614
- Nature metabolism 3:352-3652021Pre-existing beta cells but not progenitors contribute to new beta cells in the adult pancreas.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHuan Zhao,Xiuzhen Huang,Zixin Liu,Wenjuan Pu,Zan Lv,Lingjuan He,Yan Li,Qiao Zhou,Kathy O Lui,Bin ZhouPubMed 33723463
- Cell research 31:886-9032021Sequential progenitor states mark the generation of pancreatic endocrine lineages in mice and humans.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXin-Xin Yu,Wei-Lin Qiu,Liu Yang,Yan-Chun Wang,Mao-Yang He,Dan Wang,Yu Zhang,Lin-Chen Li,Jing Zhang,Yi Wang,Cheng-Ran XuPubMed 33692492
- PloS one 15:e02427492020Heterogeneous expression of CFTR in insulin-secreting β-cells of the normal human islet.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMauricio Di Fulvio et. al.PubMed 33264332
- Cancer research 80:3620-36302020Rb and p53 Execute Distinct Roles in the Development of Pancreatic Neuroendocrine Tumors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuki Yamauchi et. al.PubMed 32591410
- Life science alliance 3:2020Pancreatic cancer triggers diabetes through TGF-β-mediated selective depletion of islet β-cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesParash Parajuli et. al.PubMed 32371554
- Molecular and cellular endocrinology 510:1108152020Syndecan-4 is regulated by IL-1β in β-cells and human islets.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEstelle Brioudes et. al.PubMed 32315719
- Endocrine journal 66:459-4682019Development of monoclonal mouse antibodies that specifically recognize pancreatic polypeptide.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAkemi Hara et. al.PubMed 30842364
- Cell metabolism 29:769-783.e42019Multiplexed In Situ Imaging Mass Cytometry Analysis of the Human Endocrine Pancreas and Immune System in Type 1 Diabetes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYue J Wang et. al.PubMed 30713110
Key facts
- Isotype
IgG
- Host species
Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Synthetic Peptide within Human Pancreatic prohormone aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P01298
Consider this alternative
Reactivity data
Select an application| WB | ELISA | ICC/IF | IHC-P | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Expected | Expected | Expected | Tested | Tested |
| Mouse | Expected | Expected | Expected | Predicted | Predicted |
| Rat | Predicted | Predicted | Predicted | Predicted | Predicted |
| Chimpanzee | Predicted | Predicted | Predicted | Predicted | Predicted |
| Dog | Predicted | Predicted | Predicted | Predicted | Predicted |
| Macaque monkey | Predicted | Predicted | Predicted | Predicted | Predicted |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Preliminary experiments gave an approx 35-38 kDa band in human pancreas lysate, which was successfully blocked by incubation with the immunizing peptide. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 10.5 kDa. |
Species Mouse | Dilution info - | Notes Preliminary experiments gave an approx 35-38 kDa band in human pancreas lysate, which was successfully blocked by incubation with the immunizing peptide. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 10.5 kDa. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Dog, Chimpanzee, Macaque monkey | Dilution info - | Notes - |
ELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Antibody detection limit dilution 1:32,000 by peptide ELISA. |
Species Human | Dilution info - | Notes Antibody detection limit dilution 1:32,000 by peptide ELISA. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Dog, Chimpanzee, Macaque monkey | Dilution info - | Notes - |
ICC/IF
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes See Abreview. |
Species Human | Dilution info - | Notes See Abreview. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Dog, Chimpanzee, Macaque monkey | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 3-5 µg/mL | Notes In paraffin embedded human pancreas, shows strong cytoplasmic staining of rare cells located at the periphery of islets of Langerhans. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Dog, Chimpanzee, Macaque monkey | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 10 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Dog, Chimpanzee, Macaque monkey | Dilution info - | Notes - |
Associated Products
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Target data
Function
Pancreatic polypeptideHormone secreted by pancreatic cells that acts as a regulator of pancreatic and gastrointestinal functions probably by signaling through the G protein-coupled receptor NPY4R2.
Targets
Pancreatic Polypeptide
Alternative names
PNP, PNP, PPY, Pancreatic polypeptide prohormone, PH, Pancreatic polypeptide Y
Recommended products
Goat Polyclonal Pancreatic Polypeptide antibody. Suitable for WB, ELISA, ICC/IF, IHC-P, Flow Cyt (Intra) and reacts with Human, Mouse samples. Cited in 21 publications. Immunogen corresponding to Synthetic Peptide within Human Pancreatic prohormone aa 50 to C-terminus.
Key facts
- Isotype
IgG
- Host species
Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Synthetic Peptide within Human Pancreatic prohormone aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P01298
- Purification technique
Affinity purification Immunogen
- Concentration
- Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Pancreatic Polypeptide also known as PP is a small protein with a molecular mass approximately 4200 Da. It is secreted by PP cells in the pancreas particularly in the F-cells located in the Langerhans islets. This peptide is highly conserved among different species including the rat where it displays similar function and expression patterns. Pancreatic polypeptide regulates various physiological processes by binding to specific receptors on target tissues.
- Biological function summary
In various mammalian species including rats pancreatic polypeptide plays a significant role in the regulation of appetite and pancreatic secretion. It is not recognized as part of a multi-protein complex but it directly influences the function of digestive organs by inhibiting pancreatic exocrine secretions and gallbladder contraction. This makes it an important mediator in controlling the body's energy balance and digestive functions.
- Pathways
Pancreatic polypeptide is involved in the peptidergic pathway regulating energy homeostasis and digestive processes. It interacts with the Y receptor family particularly the Y4 receptor which contributes to its regulatory actions on appetite and digestive enzyme secretion. This peptide works alongside other polypeptides such as neuropeptide Y and peptide YY in mediating these complex physiological pathways.
- Associated diseases and disorders
Pancreatic polypeptide levels relate to conditions such as obesity and diabetes. Altered secretion of this peptide can contribute to obesity by affecting appetite regulation and energy homeostasis. Furthermore in diabetes pancreatic polypeptide is connected to abnormal pancreatic function often observed alongside insulin and glucagon dysregulation. Understanding these relationships can provide insight into therapeutic strategies targeting metabolic and endocrine disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pancreatic Polypeptide antibody (ab77192)
Staining of formalin-fixed, paraffin-embedded human pancreas tissue with 3μg/ml ab77192. Note strong cytoplasmic staining of rare cells located at the periphery of islets of Langerhans.
Microwaved antigen retrieval with Tris/EDTA buffer pH6, HRP-staining.
Flow Cytometry (Intracellular) - Anti-Pancreatic Polypeptide antibody (ab77192)
Flow cytometric analysis of paraformaldehyde fixed U2OS cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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