AB77192

Anti-Pancreatic Polypeptide antibody

Name: Anti-Pancreatic Polypeptide antibody (ab77192) | Abcam
Brand: Abcam Limited
SKU: AB77192
Price: 515 USD
Availability: InStock
Rating: 3 (3 reviews)

(3 Reviews)

(24 Publications)

Goat Polyclonal Pancreatic Polypeptide antibody. Suitable for WB, ELISA, ICC/IF, IHC-P, Flow Cyt (Intra) and reacts with Human, Mouse samples. Cited in 24 publications. Immunogen corresponding to Synthetic Peptide within Human PPY aa 50 to C-terminus.

View Alternative Names

PNP, PPY, Pancreatic polypeptide prohormone, PH, Pancreatic polypeptide Y

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pancreatic Polypeptide antibody (AB77192)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pancreatic Polypeptide antibody (AB77192)

Staining of formalin-fixed, paraffin-embedded human pancreas tissue with 3μg/ml ab77192. Note strong cytoplasmic staining of rare cells located at the periphery of islets of Langerhans.

Microwaved antigen retrieval with Tris/EDTA buffer pH6, HRP-staining.

Flow Cytometry (Intracellular) - Anti-Pancreatic Polypeptide antibody (AB77192)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Pancreatic Polypeptide antibody (AB77192)

Flow cytometric analysis of paraformaldehyde fixed U2OS cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control : Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

Flow Cyt (Intra), IHC-P, ICC/IF, ELISA, WB

applications

Immunogen

Synthetic Peptide within Human PPY aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P01298

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Reactivity data

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Pancreatic Polypeptide also known as PP is a small protein with a molecular mass approximately 4200 Da. It is secreted by PP cells in the pancreas particularly in the F-cells located in the Langerhans islets. This peptide is highly conserved among different species including the rat where it displays similar function and expression patterns. Pancreatic polypeptide regulates various physiological processes by binding to specific receptors on target tissues.

Biological function summary

In various mammalian species including rats pancreatic polypeptide plays a significant role in the regulation of appetite and pancreatic secretion. It is not recognized as part of a multi-protein complex but it directly influences the function of digestive organs by inhibiting pancreatic exocrine secretions and gallbladder contraction. This makes it an important mediator in controlling the body's energy balance and digestive functions.

Pathways

Pancreatic polypeptide is involved in the peptidergic pathway regulating energy homeostasis and digestive processes. It interacts with the Y receptor family particularly the Y4 receptor which contributes to its regulatory actions on appetite and digestive enzyme secretion. This peptide works alongside other polypeptides such as neuropeptide Y and peptide YY in mediating these complex physiological pathways.

Pancreatic polypeptide levels relate to conditions such as obesity and diabetes. Altered secretion of this peptide can contribute to obesity by affecting appetite regulation and energy homeostasis. Furthermore in diabetes pancreatic polypeptide is connected to abnormal pancreatic function often observed alongside insulin and glucagon dysregulation. Understanding these relationships can provide insight into therapeutic strategies targeting metabolic and endocrine disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Pancreatic polypeptide. Hormone secreted by pancreatic cells that acts as a regulator of pancreatic and gastrointestinal functions probably by signaling through the G protein-coupled receptor NPY4R2.

See full target information PPY

Additional targets

Pancreatic Polypeptide

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Publications (24)

Recent publications for all applications. Explore the full list and refine your search

BMC genomics 25:427 PubMed38689254

2024

NKX2-2 based nuclei sorting on frozen human archival pancreas enables the enrichment of islet endocrine populations for single-nucleus RNA sequencing.

Applications

Unspecified application

Species

Unspecified reactive species

Gengqiang Xie,Maria Pilar Toledo,Xue Hu,Hyo Jeong Yong,Pamela Sandoval Sanchez,Chengyang Liu,Ali Naji,Jerome Irianto,Yue J Wang

iScience 26:106500 PubMed37096042

2023

Loss of Fgf9 in mice leads to pancreatic hypoplasia and asplenia.

Applications

Unspecified application

Species

Unspecified reactive species

Sophie Patzek,Zhe Liu,Sean de la O,Sean Chang,Lauren E Byrnes,Xiuqin Zhang,David M Ornitz,Julie B Sneddon

Cell reports 37:109919 PubMed34731614

2021

Single-cell analysis of the human pancreas in type 2 diabetes using multi-spectral imaging mass cytometry.

Applications

Unspecified application

Species

Unspecified reactive species

Minghui Wu,Michelle Y Y Lee,Varun Bahl,Daniel Traum,Jonathan Schug,Irina Kusmartseva,Mark A Atkinson,Guanjie Fan,Klaus H Kaestner

Nature metabolism 3:352-365 PubMed33723463

2021

Pre-existing beta cells but not progenitors contribute to new beta cells in the adult pancreas.

Applications

Unspecified application

Species

Unspecified reactive species

Huan Zhao,Xiuzhen Huang,Zixin Liu,Wenjuan Pu,Zan Lv,Lingjuan He,Yan Li,Qiao Zhou,Kathy O Lui,Bin Zhou

Cell research 31:886-903 PubMed33692492

2021

Sequential progenitor states mark the generation of pancreatic endocrine lineages in mice and humans.

Applications

Unspecified application

Species

Unspecified reactive species

Xin-Xin Yu,Wei-Lin Qiu,Liu Yang,Yan-Chun Wang,Mao-Yang He,Dan Wang,Yu Zhang,Lin-Chen Li,Jing Zhang,Yi Wang,Cheng-Ran Xu

PloS one 15:e0242749 PubMed33264332

2020

Heterogeneous expression of CFTR in insulin-secreting β-cells of the normal human islet.

Applications

Unspecified application

Species

Unspecified reactive species

Mauricio Di Fulvio,Marika Bogdani,Myrian Velasco,Timothy S McMillen,Cecilia Ridaura,Lisa Kelly,Mohammed M Almutairi,Shams Kursan,Abu A Sajib,Marcia Hiriart,Lydia Aguilar-Bryan

Cancer research 80:3620-3630 PubMed32591410

2020

Rb and p53 Execute Distinct Roles in the Development of Pancreatic Neuroendocrine Tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Yuki Yamauchi,Yuzo Kodama,Masahiro Shiokawa,Nobuyuki Kakiuchi,Saiko Marui,Takeshi Kuwada,Yuko Sogabe,Teruko Tomono,Atsushi Mima,Toshihiro Morita,Tomoaki Matsumori,Tatsuki Ueda,Motoyuki Tsuda,Yoshihiro Nishikawa,Katsutoshi Kuriyama,Yojiro Sakuma,Yuji Ota,Takahisa Maruno,Norimitsu Uza,Atsuhiro Masuda,Hisato Tatsuoka,Daisuke Yabe,Sachiko Minamiguchi,Toshihiko Masui,Nobuya Inagaki,Shinji Uemoto,Tsutomu Chiba,Hiroshi Seno

Cell transplantation 29:963689720927392 PubMed32584149

2020

Long-Term Liraglutide Administration Induces Pancreas Neogenesis in Adult T2DM Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Hongjun Deng,Fengying Yang,Xiaoyi Ma,Ying Wang,Qi Chen,Li Yuan

Life science alliance 3: PubMed32371554

2020

Pancreatic cancer triggers diabetes through TGF-β-mediated selective depletion of islet β-cells.

Applications

Unspecified application

Species

Unspecified reactive species

Parash Parajuli,Thien Ly Nguyen,Céline Prunier,Mohammed S Razzaque,Keli Xu,Azeddine Atfi

Molecular and cellular endocrinology 510:110815 PubMed32315719

2020

Syndecan-4 is regulated by IL-1β in β-cells and human islets.

Applications

Unspecified application

Species

Unspecified reactive species

Estelle Brioudes,Mohamed Alibashe-Ahmed,Vanessa Lavallard,Thierry Berney,Domenico Bosco

View all publications

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Product promise

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Anti-Pancreatic Polypeptide antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pancreatic Polypeptide antibody (AB77192)

Flow Cytometry (Intracellular) - Anti-Pancreatic Polypeptide antibody (AB77192)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (24)

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