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Mouse Monoclonal PANK2 antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Rat, African green monkey, Dog, Transfected cell line - Human, Recombinant full length protein samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human PANK2.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PANK2 antibody [OTI3H9] (AB119070), expandable thumbnail
  • Western blot - Anti-PANK2 antibody [OTI3H9] (AB119070), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-PANK2 antibody [OTI3H9] (AB119070), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-PANK2 antibody [OTI3H9] (AB119070), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PANK2 antibody [OTI3H9] (AB119070), expandable thumbnail

Key facts

Isotype

IgG2a

Host species

Mouse

Storage buffer

pH: 7
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Recombinant Full Length Protein corresponding to Human PANK2. Database link Q9BZ23

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Expected
Tested
Rat
Expected
Tested
Expected
Expected
African green monkey
Expected
Tested
Expected
Expected
Dog
Expected
Tested
Expected
Expected
Recombinant full length protein
Not recommended
Not recommended
Not recommended
Tested
Transfected cell line - Human
Not recommended
Not recommended
Tested
Not recommended

Tested
Tested

Species

Human

Dilution info
1/50
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Expected
Expected

Species

Rat, African green monkey, Dog

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Transfected cell line - Human, Recombinant full length protein

Dilution info

-

Notes

-

Tested
Tested

Species

Rat

Dilution info
1/1000.00000 - 1/2000.00000
Notes

-

Species

African green monkey

Dilution info
1/1000.00000 - 1/2000.00000
Notes

-

Species

Human

Dilution info
1/1000.00000 - 1/2000.00000
Notes

-

Species

Dog

Dilution info
1/1000.00000 - 1/2000.00000
Notes

-

Not recommended
Not recommended

Species

Transfected cell line - Human, Recombinant full length protein

Dilution info

-

Notes

-

Tested
Tested

Species

Transfected cell line - Human

Dilution info
1/100
Notes

-

Expected
Expected

Species

Human, Rat, African green monkey, Dog

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Recombinant full length protein

Dilution info

-

Notes

-

Tested
Tested

Species

Recombinant full length protein

Dilution info
1/100
Notes

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Species

Human

Dilution info
1/100
Notes

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species

Rat, African green monkey, Dog

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Transfected cell line - Human

Dilution info

-

Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Isoform 1Mitochondrial isoform that catalyzes the phosphorylation of pantothenate to generate 4'-phosphopantothenate in the first and rate-determining step of coenzyme A (CoA) synthesis (PubMed:15659606, PubMed:16272150, PubMed:17242360, PubMed:17825826). Required for angiogenic activity of umbilical vein of endothelial cells (HUVEC) (PubMed:30221726).Isoform 4Cytoplasmic isoform that catalyzes the phosphorylation of pantothenate to generate 4'-phosphopantothenate in the first and rate-determining step of coenzyme A (CoA) synthesis.

Alternative names

C20orf48, PANK2, hPanK2, Pantothenic acid kinase 2

Recommended products

Mouse Monoclonal PANK2 antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Rat, African green monkey, Dog, Transfected cell line - Human, Recombinant full length protein samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human PANK2.

Key facts

Isotype

IgG2a

Form

Liquid

Clonality

Monoclonal

Immunogen
  • Recombinant Full Length Protein corresponding to Human PANK2. Database link Q9BZ23
Clone number

OTI3H9

Purification technique

Affinity purification

Concentration
Loading...
Purification notes

Purified from cell culture supernatant by affinity chromatography.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Clone OTI3H9 (formerly 3H9).

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

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Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PANK2 antibody [OTI3H9] (ab119070), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PANK2 antibody [OTI3H9] (ab119070)

    Paraffin-embedded human liver tissue stained for PANK2 with ab119070 at a 1/50 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.

  • Western blot - Anti-PANK2 antibody [OTI3H9] (ab119070), expandable thumbnail

    Western blot - Anti-PANK2 antibody [OTI3H9] (ab119070)

    All lanes: Western blot - Anti-PANK2 antibody [OTI3H9] (ab119070) at 1/1000 dilution

    Lane 1: HepG2 (Human liver hepatocellular carcinoma cell line) cell extract at 35 µg

    Lane 2: HeLa (Human epithelial cell line from cervix adenocarcinoma) cell extract at 35 µg

    Lane 3: HT-29 (Human colorectal adenocarcinoma cell line) cell extract at 35 µg

    Lane 4: A549 (Human lung carcinoma cell line) cell extract at 35 µg

    Lane 5: COS-7 (African green monkey kidney fibroblast-like cell line) cell extract at 35 µg

    Lane 6: Jurkat (Human T cell leukemia cell line from peripheral blood) cell extract at 35 µg

    Lane 7: MDCK (Canine kidney cell line) cell extract at 35 µg

    Lane 8: PC-12 (Rat adrenal gland pheochromocytoma cell line) cell extract at 35 µg

    Lane 9: MCF7 (Human breast adenocarcinoma cell line) cell extract at 35 µg

    Predicted band size: 39 kDa, 63 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-PANK2 antibody [OTI3H9] (ab119070), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-PANK2 antibody [OTI3H9] (ab119070)

    pCMV6-ENTRY PANK2 cDNA transfected COS-7 (African green monkey kidney fibroblast-like cell line) cells stained for PANK2 using ab119070 at a 1/100 dilution in ICC/IF.

  • Flow Cytometry (Intracellular) - Anti-PANK2 antibody [OTI3H9] (ab119070), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-PANK2 antibody [OTI3H9] (ab119070)

    ab119070 at 1/100 dilution staining PANK2 in Jurkat (Human T cell leukemia cell line from peripheral blood) cells by Flow cytometry (Intracellular) (Red) compared to a nonspecific negative control antibody (Blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PANK2 antibody [OTI3H9] (ab119070), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PANK2 antibody [OTI3H9] (ab119070)

    Paraffin-embedded human prostate tissue stained for PANK2 with ab119070 at a 1/50 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PANK2 antibody [OTI3H9] (ab119070), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PANK2 antibody [OTI3H9] (ab119070)

    Paraffin-embedded human prostate carcinoma tissue stained for PANK2 with ab119070 at a 1/50 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.

  • Flow Cytometry (Intracellular) - Anti-PANK2 antibody [OTI3H9] (ab119070), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-PANK2 antibody [OTI3H9] (ab119070)

    ab119070 at 1/100 dilution staining PANK2 in HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with either pCMV6-ENTRY PANK2 overexpress plasmid (Red) or empty vector control plasmid (Blue) by Flow Cytometry (Intracellular).

  • Western blot - Anti-PANK2 antibody [OTI3H9] (ab119070), expandable thumbnail

    Western blot - Anti-PANK2 antibody [OTI3H9] (ab119070)

    All lanes: Western blot - Anti-PANK2 antibody [OTI3H9] (ab119070) at 1/1000 dilution

    Lane 1: HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control cDNA at 5 µg

    Lane 2: HEK-293T cell lysate transfected with pCMV6-ENTRY PANK2 cDNA at 5 µg

    Predicted band size: 63 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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