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AB119070

Anti-PANK2 antibody [OTI3H9]

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(3 Publications)

Mouse Monoclonal PANK2 antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Rat, African green monkey, Dog, Transfected cell line - Human, Recombinant full length protein samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human PANK2.

View Alternative Names

C20orf48, PANK2, hPanK2, Pantothenic acid kinase 2

8 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PANK2 antibody [OTI3H9] (AB119070)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PANK2 antibody [OTI3H9] (AB119070)

Paraffin-embedded human prostate carcinoma tissue stained for PANK2 with ab119070 at a 1/50 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.

Flow Cytometry (Intracellular) - Anti-PANK2 antibody [OTI3H9] (AB119070)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-PANK2 antibody [OTI3H9] (AB119070)

ab119070 at 1/100 dilution staining PANK2 in Jurkat (Human T cell leukemia cell line from peripheral blood) cells by Flow cytometry (Intracellular) (Red) compared to a nonspecific negative control antibody (Blue).

Flow Cytometry (Intracellular) - Anti-PANK2 antibody [OTI3H9] (AB119070)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-PANK2 antibody [OTI3H9] (AB119070)

ab119070 at 1/100 dilution staining PANK2 in HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with either pCMV6-ENTRY PANK2 overexpress plasmid (Red) or empty vector control plasmid (Blue) by Flow Cytometry (Intracellular).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PANK2 antibody [OTI3H9] (AB119070)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PANK2 antibody [OTI3H9] (AB119070)

Paraffin-embedded human liver tissue stained for PANK2 with ab119070 at a 1/50 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PANK2 antibody [OTI3H9] (AB119070)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PANK2 antibody [OTI3H9] (AB119070)

Paraffin-embedded human prostate tissue stained for PANK2 with ab119070 at a 1/50 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.

Immunocytochemistry/ Immunofluorescence - Anti-PANK2 antibody [OTI3H9] (AB119070)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PANK2 antibody [OTI3H9] (AB119070)

pCMV6-ENTRY PANK2 cDNA transfected COS-7 (African green monkey kidney fibroblast-like cell line) cells stained for PANK2 using ab119070 at a 1/100 dilution in ICC/IF.

Western blot - Anti-PANK2 antibody [OTI3H9] (AB119070)
  • WB

Unknown

Western blot - Anti-PANK2 antibody [OTI3H9] (AB119070)

All lanes:

Western blot - Anti-PANK2 antibody [OTI3H9] (ab119070) at 1/1000 dilution

Lane 1:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control cDNA at 5 µg

Lane 2:

HEK-293T cell lysate transfected with pCMV6-ENTRY PANK2 cDNA at 5 µg

Predicted band size: 63 kDa

false

Western blot - Anti-PANK2 antibody [OTI3H9] (AB119070)
  • WB

Unknown

Western blot - Anti-PANK2 antibody [OTI3H9] (AB119070)

All lanes:

Western blot - Anti-PANK2 antibody [OTI3H9] (ab119070) at 1/1000 dilution

Lane 1:

HepG2 (Human liver hepatocellular carcinoma cell line) cell extract at 35 µg

Lane 2:

HeLa (Human epithelial cell line from cervix adenocarcinoma) cell extract at 35 µg

Lane 3:

HT-29 (Human colorectal adenocarcinoma cell line) cell extract at 35 µg

Lane 4:

A549 (Human lung carcinoma cell line) cell extract at 35 µg

Lane 5:

COS-7 (African green monkey kidney fibroblast-like cell line) cell extract at 35 µg

Lane 6:

Jurkat (Human T cell leukemia cell line from peripheral blood) cell extract at 35 µg

Lane 7:

MDCK (Canine kidney cell line) cell extract at 35 µg

Lane 8:

PC-12 (Rat adrenal gland pheochromocytoma cell line) cell extract at 35 µg

Lane 9:

MCF7 (Human breast adenocarcinoma cell line) cell extract at 35 µg

Predicted band size: 39 kDa,63 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

OTI3H9

Isotype

IgG2a

Carrier free

No

Reacts with

Rat, Dog, Human, African green monkey

Applications

Flow Cyt (Intra), ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human PANK2.

Q9BZ23

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg2c-kappa-monoclonal-18c8bc7ad10-isotype-control-ab170191'>ab170191</a> - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.</p>" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "African green monkey": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Dog": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Recombinant full length protein": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg2c-kappa-monoclonal-18c8bc7ad10-isotype-control-ab170191'>ab170191</a> - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.</p>" }, "Transfected cell line - Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

Clone OTI3H9 (formerly 3H9).
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Purification notes
Purified from cell culture supernatant by affinity chromatography.
Storage buffer
pH: 7 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Isoform 1. Mitochondrial isoform that catalyzes the phosphorylation of pantothenate to generate 4'-phosphopantothenate in the first and rate-determining step of coenzyme A (CoA) synthesis (PubMed : 15659606, PubMed : 16272150, PubMed : 17242360, PubMed : 17825826). Required for angiogenic activity of umbilical vein of endothelial cells (HUVEC) (PubMed : 30221726).. Isoform 4. Cytoplasmic isoform that catalyzes the phosphorylation of pantothenate to generate 4'-phosphopantothenate in the first and rate-determining step of coenzyme A (CoA) synthesis.
See full target information PANK2
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Antioxidants (Basel, Switzerland) 14: PubMed40002401

2025

Polydatin and Nicotinamide Prevent Iron Accumulation and Lipid Peroxidation in Cellular Models of Mitochondrial Diseases.

Applications

Unspecified application

Species

Unspecified reactive species

Paula Cilleros-Holgado,David Gómez-Fernández,Rocío Piñero-Pérez,José Manuel Romero-Domínguez,Diana Reche-López,Mónica Álvarez-Córdoba,Ana Romero-González,Alejandra López-Cabrera,Marta Castro De Oliveira,Andrés Rodríguez-Sacristán,Susana González-Granero,José Manuel García-Verdugo,José Antonio Sánchez-Alcázar

PLoS genetics 16:e1009020 PubMed32976492

2020

Deficiency of the Tbc1d21 gene causes male infertility with morphological abnormalities of the sperm mitochondria and flagellum in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Ya-Yun Wang,Chih-Chun Ke,Yen-Lin Chen,Yu-Hua Lin,I-Shing Yu,Wei-Chi Ku,Moira K O'Bryan,Ying-Hung Lin

PloS one 12:e0184104 PubMed28863176

2017

iPSC-derived neuronal models of PANK2-associated neurodegeneration reveal mitochondrial dysfunction contributing to early disease.

Applications

Unspecified application

Species

Unspecified reactive species

Charles Arber,Plamena R Angelova,Sarah Wiethoff,Yugo Tsuchiya,Francesca Mazzacuva,Elisavet Preza,Kailash P Bhatia,Kevin Mills,Ivan Gout,Andrey Y Abramov,John Hardy,James A Duce,Henry Houlden,Selina Wray
View all publications
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