AB233479

Anti-Pannexin 1 antibody

Name: Anti-Pannexin 1 antibody (ab233479) | Abcam
Brand: Abcam Limited
SKU: AB233479

Be the first to review this product! Submit a review

(2 Publications)

Rabbit Polyclonal Pannexin 1 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PANX1 aa 200 to C-terminus.

View Alternative Names

MRS1, UNQ2529/PRO6028, PANX1, Pannexin-1

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pannexin 1 antibody (AB233479)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pannexin 1 antibody (AB233479)

Paraffin-embedded human kidney tissue stained for Pannexin 1 using ab233479 at 20 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pannexin 1 antibody (AB233479)

Paraffin-embedded human liver cancer tissue stained for Pannexin 1 using ab233479 at 20 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pannexin 1 antibody (AB233479)

Paraffin-embedded human liver tissue stained for Pannexin 1 using ab233479 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Supplier Data

Western blot - Anti-Pannexin 1 antibody (AB233479)

All lanes:

Western blot - Anti-Pannexin 1 antibody (ab233479) at 3 µg/mL

Lane 1:

SK-N-SH (human neuroblastoma cell line) cell lysate

Lane 2:

U-87 MG (human glioblastoma-astrocytoma epithelial cell line) cell lysate

Secondary

All lanes:

HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution

Predicted band size: 48 kDa

false

Supplier Data

Western blot - Anti-Pannexin 1 antibody (AB233479)

All lanes:

Western blot - Anti-Pannexin 1 antibody (ab233479) at 3 µg/mL

All lanes:

Recombinant human Pannexin 1 protein

Secondary

All lanes:

HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution

Predicted band size: 48 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human PANX1 aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q96RD7

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-5 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "<p></p>" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab233479 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Pannexin 1 also known as PANX1 is a membrane channel protein with a mass of approximately 48 kDa. This protein functions as an important part of the cellular communication system by forming channels that facilitate the release of ATP and other signaling molecules from the cytoplasm to the extracellular space. It is widely expressed across various tissues including the central nervous system skin and muscles. Pannexin-1 is often found on the cell surface where it operates in coordination with other cellular components to regulate intercellular interactions and signaling networks.

Biological function summary

Pannexin 1 plays a significant role in various physiological processes such as inflammation apoptosis and synaptic plasticity. It forms part of large multiprotein complexes that can include connexins and other ion channels. Through these complexes pannexin 1 helps mediate the flow of ions and small molecules across the cell membrane influencing cellular responses and communication. The ATP release mediated by pannexin-1 channels is particularly involved in activating purinergic receptors on neighboring cells contributing to diverse cellular responses.

Pathways

Pannexin 1 participates actively in the purinergic signaling pathway where it plays a role in ATP mediated communication between cells. This protein also integrates into the inflammasome pathway impacting inflammation and immune responses. Within these pathways pannexin-1 interacts with proteins such as P2X7 receptors which are essential for the activation of specific signaling cascades linked to these biological processes. The interaction with other channels like connexins enhances its regulatory role in physiological functions.

Pannexin 1 has been implicated in the pathophysiology of conditions such as epilepsy and skin disorders. Its ability to control ATP release and consequently influence neuron excitability relates significantly to the onset and progression of epilepsy. PANX1 also interacts with cytokines in skin cells where dysregulation can contribute to inflammatory skin conditions. Additionally alterations in pannexin-1 function and expression have connections to changes in P2X7 receptor activities linking it to both neurological and dermatological disease pathways.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Ion channel involved in a variety of physiological functions such as blood pressure regulation, apoptotic cell clearance and oogenesis (PubMed : 15304325, PubMed : 16908669, PubMed : 20829356, PubMed : 20944749, PubMed : 30918116). Forms anion-selective channels with relatively low conductance and an order of permeabilities : nitrate>iodide>chlroride>>aspartate=glutamate=gluconate (By similarity). Can release ATP upon activation through phosphorylation or cleavage at C-terminus (PubMed : 32238926). May play a role as a Ca(2+)-leak channel to regulate ER Ca(2+) homeostasis (PubMed : 16908669).. Caspase-activated pannexin-1. During apoptosis, the C terminal tail is cleaved by caspases, which opens the main pore acting as a large-pore ATP efflux channel with a broad distribution, which allows the regulated release of molecules and ions smaller than 1 kDa, such as nucleotides ATP and UTP, and selective plasma membrane permeability to attract phagocytes that engulf the dying cells.

See full target information PANX1

style

left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 15:24 PubMed38195677

2024

TNFα modulates PANX1 activation to promote ATP release and enhance P2RX7-mediated antitumor immune responses after chemotherapy in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Kevin Chih-Yang Huang,Shu-Fen Chiang,Pei-Chun Lin,Wei-Ze Hong,Pei-Chen Yang,Hui-Ping Chang,Shin-Lei Peng,Tsung-Wei Chen,Tao-Wei Ke,Ji-An Liang,William Tzu-Liang Chen,K S Clifford Chao

Oncology letters 16:6355-6360 PubMed30405771

2018

miR-145-5p inhibits the proliferation and migration of bladder cancer cells by targeting TAGLN2.

Applications

Unspecified application

Species

Unspecified reactive species

Haijian Zhang,Meijuan Jiang,Qingjun Liu,Zhixing Han,Yuqian Zhao,Shiqi Ji

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Pannexin 1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pannexin 1 antibody (AB233479)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pannexin 1 antibody (AB233479)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pannexin 1 antibody (AB233479)

Western blot - Anti-Pannexin 1 antibody (AB233479)

Western blot - Anti-Pannexin 1 antibody (AB233479)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise