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AB308039

Anti-Pannexin 1 antibody [RP23040037]

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Rabbit Recombinant Multiclonal Pannexin 1 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human PANX1.

View Alternative Names

MRS1, UNQ2529/PRO6028, PANX1, Pannexin-1

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-Pannexin 1 antibody [RP23040037] (AB308039)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Pannexin 1 antibody [RP23040037] (AB308039)

For immunofluorescence analysis, HEK293 cells were treated with ab308039 for detection of Pannexin-1 followed by labeling with Alexa Fluor 488®-conjugated Goat anti-Rabbit secondary antibody (panel a : green). DAPI stained nucleus (panel b : blue) Alexa Fluor 594® phalloidin stained actin (panel b : red) Panel c : Composite image of cells showing cell-membrane and junction localization of Pannexin 1

Western blot - Anti-Pannexin 1 antibody [RP23040037] (AB308039)
  • WB

Supplier Data

Western blot - Anti-Pannexin 1 antibody [RP23040037] (AB308039)

Western blot analysis of Pannexin 1 in whole cell extracts of MCF-7 using ab308039 at a dilution of 2 µg/mL. Samples were detected using chemiluminescence (ECL). Results show a band at ~48kDa.

All lanes:

Western blot - Anti-Pannexin 1 antibody [RP23040037] (ab308039) at 2 µg/mL

All lanes:

MCF-7 whole cell extracts at 2 µg/mL

Predicted band size: 48 kDa

Observed band size: 48 kDa

true

Key facts

Host species

Rabbit

Clonality

Multiclonal

Clone number

RP23040037

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human PANX1.

Q96RD7

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "2 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1-3 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Product details

What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:

  • - The sensitivity of polyclonal antibodies by recognising multiple epitopes
  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

View our range of recombinant multiclonal antibodies.

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Properties and storage information

Form
Liquid
Storage buffer
Preservative: 0.09% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Pannexin 1 also known as PANX1 is a membrane channel protein with a mass of approximately 48 kDa. This protein functions as an important part of the cellular communication system by forming channels that facilitate the release of ATP and other signaling molecules from the cytoplasm to the extracellular space. It is widely expressed across various tissues including the central nervous system skin and muscles. Pannexin-1 is often found on the cell surface where it operates in coordination with other cellular components to regulate intercellular interactions and signaling networks.
Biological function summary

Pannexin 1 plays a significant role in various physiological processes such as inflammation apoptosis and synaptic plasticity. It forms part of large multiprotein complexes that can include connexins and other ion channels. Through these complexes pannexin 1 helps mediate the flow of ions and small molecules across the cell membrane influencing cellular responses and communication. The ATP release mediated by pannexin-1 channels is particularly involved in activating purinergic receptors on neighboring cells contributing to diverse cellular responses.

Pathways

Pannexin 1 participates actively in the purinergic signaling pathway where it plays a role in ATP mediated communication between cells. This protein also integrates into the inflammasome pathway impacting inflammation and immune responses. Within these pathways pannexin-1 interacts with proteins such as P2X7 receptors which are essential for the activation of specific signaling cascades linked to these biological processes. The interaction with other channels like connexins enhances its regulatory role in physiological functions.

Pannexin 1 has been implicated in the pathophysiology of conditions such as epilepsy and skin disorders. Its ability to control ATP release and consequently influence neuron excitability relates significantly to the onset and progression of epilepsy. PANX1 also interacts with cytokines in skin cells where dysregulation can contribute to inflammatory skin conditions. Additionally alterations in pannexin-1 function and expression have connections to changes in P2X7 receptor activities linking it to both neurological and dermatological disease pathways.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Ion channel involved in a variety of physiological functions such as blood pressure regulation, apoptotic cell clearance and oogenesis (PubMed : 15304325, PubMed : 16908669, PubMed : 20829356, PubMed : 20944749, PubMed : 30918116). Forms anion-selective channels with relatively low conductance and an order of permeabilities : nitrate>iodide>chlroride>>aspartate=glutamate=gluconate (By similarity). Can release ATP upon activation through phosphorylation or cleavage at C-terminus (PubMed : 32238926). May play a role as a Ca(2+)-leak channel to regulate ER Ca(2+) homeostasis (PubMed : 16908669).. Caspase-activated pannexin-1. During apoptosis, the C terminal tail is cleaved by caspases, which opens the main pore acting as a large-pore ATP efflux channel with a broad distribution, which allows the regulated release of molecules and ions smaller than 1 kDa, such as nucleotides ATP and UTP, and selective plasma membrane permeability to attract phagocytes that engulf the dying cells.
See full target information PANX1
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Product promise

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