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AB237055

Anti-Pannexin 3 antibody

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(2 Publications)

Rabbit Polyclonal Pannexin 3 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Pannexin-3 aa 100-250.

View Alternative Names

Pannexin-3, PANX3

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-Pannexin 3 antibody (AB237055)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Pannexin 3 antibody (AB237055)

HepG2 (Human liver hepatocellular carcinoma cell line) cells stained for Pannexin 3 (green) using ab237055 at 1/133 dilution in ICC/IF, followed by an Alexa-Fluor®488-conjugated goat anti-rabbit IgG (H+L). Counterstained with DAPI (blue).

The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the antibody overnight at 4°C.

Western blot - Anti-Pannexin 3 antibody (AB237055)
  • WB

Supplier Data

Western blot - Anti-Pannexin 3 antibody (AB237055)

All lanes:

Western blot - Anti-Pannexin 3 antibody (ab237055) at 1/500 dilution

Lane 1:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate

Lane 2:

HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 45 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human Pannexin-3 aa 100-250. The exact immunogen used to generate this antibody is proprietary information.

Q96QZ0

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Pannexin 3 also known as Panx3 is a part of the pannexin family of proteins and consists of approximately 43 kDa in mass. Mechanically Panx3 forms large-pore channels that facilitate the passage of ions and small molecules across the cell membrane contributing to intercellular communication. This protein is expressed not only in a range of tissues such as the skin bone and cartilage but also in organs like the heart and liver signaling its widespread influence in the body.
Biological function summary

The protein functions at the cellular level include regulation of calcium signaling and ATP release which influences various cell functions. Panx3 often exists as a part of complexes with other similar proteins like connexins which play roles in forming gap junctions. These activities are essential in controlling cellular interactions and maintaining tissue function particularly in supporting cartilage and bone homeostasis.

Pathways

Panx3 influences critical biological processes including the Wnt and PI3K-AKT pathways which are significant in cell proliferation differentiation and survival. Within these pathways Panx3 interacts with proteins such as β-catenin in the Wnt signaling and AKT in the PI3K-AKT pathway. Its activities in these pathways underline its involvement in cellular growth and development processes.

Panx3 has been linked to osteoarthritis and certain cardiac dysfunctions. In osteoarthritis the dysregulation of Panx3 impacts bone and cartilage maintenance as it interacts with proteins such as MMPs (matrix metalloproteinases) that contribute to cartilage breakdown. In cardiac dysfunctions the association of Panx3 with connexins may implicate it in irregular heart signaling. These connections suggest that alterations in this protein's expression or function may lead to pathological conditions in tissues where it is expressed.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Regulator of osteoblast differentiation by functionning as a Ca(2+) channel in the endoplasmic reticulum which regulates calmodulin (CaM) pathways. Allows ATP release into the extracellular space and activation or purinergic receptors.
See full target information Pannexin-3
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature cell biology 25:1774-1786 PubMed37957325

2023

NAD regulates nucleotide metabolism and genomic DNA replication.

Applications

Unspecified application

Species

Unspecified reactive species

Sebastian Howen Nesgaard Munk,Joanna Maria Merchut-Maya,Alba Adelantado Rubio,Arnaldur Hall,George Pappas,Giacomo Milletti,MyungHee Lee,Lea Giørtz Johnsen,Per Guldberg,Jiri Bartek,Apolinar Maya-Mendoza

EXCLI journal 21:1111-1129 PubMed36381643

2022

Expression of connexins and pannexins in diseased human liver.

Applications

Unspecified application

Species

Unspecified reactive species

Kaat Leroy,Vânia Vilas-Boas,Eva Gijbels,Bart Vanderborght,Lindsey Devisscher,Bruno Cogliati,Bert Van Den Bossche,Isabelle Colle,Mathieu Vinken
View all publications
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