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Rabbit Recombinant Monoclonal PAPP A antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 1 publication.

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Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPWBICC/IF
Human
Tested
Tested
Tested
Not recommended

Tested
Tested

Species

Human

Dilution info

1/100 - 1/250

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species

Human

Dilution info

1/10 - 1/100

Notes

-

Tested
Tested

Species

Human

Dilution info

1/1000 - 1/10000

Notes

-

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

-

Target data

Function

Metalloproteinase which specifically cleaves IGFBP-4 and IGFBP-5, resulting in release of bound IGF. Cleavage of IGFBP-4 is dramatically enhanced by the presence of IGF, whereas cleavage of IGFBP-5 is slightly inhibited by the presence of IGF.

Alternative names

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Rabbit Recombinant Monoclonal PAPP A antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 1 publication.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR12897

Purification technique

Affinity purification Protein A

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-PAPP A antibody [EPR12897] (ab174314), expandable thumbnail

    Western blot - Anti-PAPP A antibody [EPR12897] (ab174314)

    All lanes: Western blot - Anti-PAPP A antibody [EPR12897] (AB174314) at 1/1000 dilution

    All lanes: Human placenta lysate at 10 µg

    Predicted band size: 180 kDa

  • Immunoprecipitation - Anti-PAPP A antibody [EPR12897] (ab174314), expandable thumbnail

    Immunoprecipitation - Anti-PAPP A antibody [EPR12897] (ab174314)

    Western blot analysis on immunoprecipitation pellet from Human placenta lysate using ab174314 at a 1/10 dilution.

    All lanes: Immunoprecipitation - Anti-PAPP A antibody [EPR12897] (AB174314)

    Predicted band size: 180 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAPP A antibody [EPR12897] (ab174314), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAPP A antibody [EPR12897] (ab174314)

    Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling PAPP A with ab174314 at a 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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