AB174314

Anti-PAPP A antibody [EPR12897]

RabMAb
Recombinant
20ul selling size
What is this?

Be the first to review this product! Submit a review

(5 Publications)

Anti-PAPP A antibody [EPR12897] (ab174314) is a rabbit monoclonal antibody detecting PAPP A in Western Blot, IP, IHC-P. Suitable for Human.

- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

Pappalysin-1, Insulin-like growth factor-dependent IGF-binding protein 4 protease, Pregnancy-associated plasma protein A, IGF-dependent IGFBP-4 protease, IGFBP-4ase, PAPP-A, PAPPA

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAPP A antibody [EPR12897] (AB174314)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAPP A antibody [EPR12897] (AB174314)

Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling PAPP A with ab174314 at a 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Supplier Data

Immunoprecipitation - Anti-PAPP A antibody [EPR12897] (AB174314)

Western blot analysis on immunoprecipitation pellet from Human placenta lysate using ab174314 at a 1/10 dilution.

All lanes:

Immunoprecipitation - Anti-PAPP A antibody [EPR12897] (ab174314)

Predicted band size: 180 kDa

false

Supplier Data

Western blot - Anti-PAPP A antibody [EPR12897] (AB174314)

All lanes:

Western blot - Anti-PAPP A antibody [EPR12897] (ab174314) at 1/1000 dilution

All lanes:

Human placenta lysate at 10 µg

Predicted band size: 180 kDa

false

Carrier free

Anti-PAPP A antibody [EPR12897] - BSA and Azide free
660 APC

APC Anti-PAPP A antibody [EPR12897]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-PAPP A antibody [EPR12897]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-PAPP A antibody [EPR12897]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-PAPP A antibody [EPR12897]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-PAPP A antibody [EPR12897]
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-PAPP A antibody [EPR12897]
578 PE

PE Anti-PAPP A antibody [EPR12897]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12897

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

Product details

What is this antibody validated in?
Anti-PAPP A antibody [EPR12897] (ab174314) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P) in Human samples.

What is the molecular weight of PAPP A?
Anti-PAPP A [EPR12897] (ab174314) specifically detects a band for PAPP A (UniProt: Q13219) at a molecular weight of 180kDa.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR12897] also available for your convenience: ab174314, Carrier free - ab249813, APC - ab319210, PE - ab319358, Alexa Fluor® 488 - ab319492, Alexa Fluor® 647 - ab319605, Alexa Fluor® 594 - ab319709, Alexa Fluor® 555 - ab319851, Alexa Fluor® 750 - ab321178

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Metalloproteinase which specifically cleaves IGFBP-4 and IGFBP-5, resulting in release of bound IGF. Cleavage of IGFBP-4 is dramatically enhanced by the presence of IGF, whereas cleavage of IGFBP-5 is slightly inhibited by the presence of IGF.

See full target information Pappalysin-1

style

left-column

Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Cancer cell international 25:38 PubMed39920676

2025

Overexpression of metalloproteinase PAPPA accelerates cancer progression and correlates with immune cell infiltration in gastric cancer: insights from bioinformatics and in vitro investigations.

Applications

Unspecified application

Species

Unspecified reactive species

Shiya Yu,Shiyao Zheng,Jinwei Qiu,Hongming Lin,Xiaojuan Yu,Huiyan Chen,Junhong Wu,Weihang Wu,Jiong Chen,Yongyuan Chen,Jianwei Chen,Hu Zhao,Yu Wang

Nature cell biology 25:1047-1060 PubMed37400500

2023

Single-cell analysis reveals transcriptomic and epigenomic impacts on the maternal-fetal interface following SARS-CoV-2 infection.

Applications

Unspecified application

Species

Unspecified reactive species

Lin Gao,Vrinda Mathur,Sabrina Ka Man Tam,Xuemeng Zhou,Ming Fung Cheung,Lu Yan Chan,Guadalupe Estrada-Gutiérrez,Bo Wah Leung,Sakita Moungmaithong,Chi Chiu Wang,Liona C Poon,Danny Leung

Open medicine (Warsaw, Poland) 16:117-127 PubMed33542956

2021

LncRNA XIST regulates atherosclerosis progression in ox-LDL-induced HUVECs.

Applications

Unspecified application

Species

Unspecified reactive species

Hongmei Gao,Zhaohui Guo

Circulation journal : official journal of the Japanese Circulation Society 84:2259-2269 PubMed33162460

2020

Hsa-circ_0010283 Regulates Oxidized Low-Density Lipoprotein-Induced Proliferation and Migration of Vascular Smooth Muscle Cells by Targeting the miR-133a-3p/Pregnancy-Associated Plasma Protein A Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Zibo Feng,Youpeng Zhu,Jing Zhang,Wenbo Yang,Zhimin Chen,Binghui Li

Nature communications 9:3310 PubMed30120249

2018

Axoneme polyglutamylation regulated by Joubert syndrome protein ARL13B controls ciliary targeting of signaling molecules.

Applications

Unspecified application

Species

Unspecified reactive species

Kai He,Xiaoyu Ma,Tao Xu,Yan Li,Allen Hodge,Qing Zhang,Julia Torline,Yan Huang,Jian Zhao,Kun Ling,Jinghua Hu

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PAPP A antibody [EPR12897]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAPP A antibody [EPR12897] (AB174314)

Immunoprecipitation - Anti-PAPP A antibody [EPR12897] (AB174314)

Western blot - Anti-PAPP A antibody [EPR12897] (AB174314)

Related conjugates and formulations (8)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (5)

Product promise