Anti-PAPP A antibody [EPR12897] (ab174314) is a rabbit monoclonal antibody that is used to detect PAPP A in Western Blot, IP, IHC-P. Suitable for Human samples.
- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency
Images
![Western blot - Anti-PAPP A antibody [EPR12897] (AB174314), expandable thumbnail](https://content.abcam.com/products/images/papp-a-antibody-epr12897-ab174314--western-blot-img85260.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PAPP A antibody [EPR12897] (AB174314)")
![Immunoprecipitation - Anti-PAPP A antibody [EPR12897] (AB174314), expandable thumbnail](https://content.abcam.com/products/images/papp-a-antibody-epr12897-ab174314--immunoprecipitation-img72511.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-PAPP A antibody [EPR12897] (AB174314)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAPP A antibody [EPR12897] (AB174314), expandable thumbnail](https://content.abcam.com/products/images/papp-a-antibody-epr12897-ab174314--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img56795.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAPP A antibody [EPR12897] (AB174314)")
Publications
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- Open medicine (Warsaw, Poland) 16:117-1272021LncRNA XIST regulates atherosclerosis progression in ox-LDL-induced HUVECs.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHongmei Gao et. al.PubMed 33542956
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Abcam Recommends
Reactivity data
Select an application| IHC-P | IP | WB | ICC/IF | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/250 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/10 - 1/100 | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Metalloproteinase which specifically cleaves IGFBP-4 and IGFBP-5, resulting in release of bound IGF. Cleavage of IGFBP-4 is dramatically enhanced by the presence of IGF, whereas cleavage of IGFBP-5 is slightly inhibited by the presence of IGF.
Alternative names
Pappalysin-1, Insulin-like growth factor-dependent IGF-binding protein 4 protease, Pregnancy-associated plasma protein A, IGF-dependent IGFBP-4 protease, IGFBP-4ase, PAPP-A, PAPPA
Recommended products
Anti-PAPP A antibody [EPR12897] (ab174314) is a rabbit monoclonal antibody that is used to detect PAPP A in Western Blot, IP, IHC-P. Suitable for Human samples.
- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR12897
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-PAPP A antibody [EPR12897] (ab174314) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P) in Human samples.
What is the molecular weight of PAPP A?
Anti-PAPP A [EPR12897] (ab174314) specifically detects a band for PAPP A (UniProt: Q13219) at a molecular weight of 180kDa.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.
Other related products
We have a range of other formats of antibody clone [EPR12897] also available for your convenience:
ab174314, Carrier free - Anti-PAPP A antibody [EPR12897] - BSA and Azide free ab249813, APC - APC Anti-PAPP A antibody [EPR12897] ab319210, PE - PE Anti-PAPP A antibody [EPR12897] ab319358, Alexa Fluor® 488 - Alexa Fluor® 488 Anti-PAPP A antibody [EPR12897] ab319492, Alexa Fluor® 647 - Alexa Fluor® 647 Anti-PAPP A antibody [EPR12897] ab319605, Alexa Fluor® 594 - Alexa Fluor® 594 Anti-PAPP A antibody [EPR12897] ab319709, Alexa Fluor® 555 - Alexa Fluor® 555 Anti-PAPP A antibody [EPR12897] ab319851, Alexa Fluor® 750 - Alexa Fluor® 750 Anti-PAPP A antibody [EPR12897] ab321178
Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-PAPP A antibody [EPR12897] (ab174314), expandable thumbnail](https://content.abcam.com/products/images/papp-a-antibody-epr12897-ab174314--western-blot-img85260.jpg "Western blot - Anti-PAPP A antibody [EPR12897] (ab174314)")
Western blot - Anti-PAPP A antibody [EPR12897] (ab174314)
All lanes: Western blot - Anti-PAPP A antibody [EPR12897] (ab174314) at 1/1000 dilution
All lanes: Human placenta lysate at 10 µg
Predicted band size: 180 kDa
![Immunoprecipitation - Anti-PAPP A antibody [EPR12897] (ab174314), expandable thumbnail](https://content.abcam.com/products/images/papp-a-antibody-epr12897-ab174314--immunoprecipitation-img72511.jpg "Immunoprecipitation - Anti-PAPP A antibody [EPR12897] (ab174314)")
Immunoprecipitation - Anti-PAPP A antibody [EPR12897] (ab174314)
Western blot analysis on immunoprecipitation pellet from Human placenta lysate using ab174314 at a 1/10 dilution.
All lanes: Immunoprecipitation - Anti-PAPP A antibody [EPR12897] (ab174314)
Predicted band size: 180 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAPP A antibody [EPR12897] (ab174314), expandable thumbnail](https://content.abcam.com/products/images/papp-a-antibody-epr12897-ab174314--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img56795.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAPP A antibody [EPR12897] (ab174314)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAPP A antibody [EPR12897] (ab174314)
Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling PAPP A with ab174314 at a 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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