Rabbit Recombinant Monoclonal PAPP A antibody. Carrier free. Suitable for IHC-P, IP, WB and reacts with Human samples.
Images
![Western blot - Anti-PAPP A antibody [EPR12897] - BSA and Azide free (AB249813), expandable thumbnail](https://content.abcam.com/products/images/papp-a-antibody-epr12897-bsa-and-azide-free-ab249813--western-blot-img85260.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PAPP A antibody [EPR12897] - BSA and Azide free (AB249813)")
![Immunoprecipitation - Anti-PAPP A antibody [EPR12897] - BSA and Azide free (AB249813), expandable thumbnail](https://content.abcam.com/products/images/papp-a-antibody-epr12897-bsa-and-azide-free-ab249813--immunoprecipitation-img72511.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-PAPP A antibody [EPR12897] - BSA and Azide free (AB249813)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAPP A antibody [EPR12897] - BSA and Azide free (AB249813), expandable thumbnail](https://content.abcam.com/products/images/papp-a-antibody-epr12897-bsa-and-azide-free-ab249813--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img56795.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAPP A antibody [EPR12897] - BSA and Azide free (AB249813)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | WB | ICC/IF | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Target data
Function
Metalloproteinase which specifically cleaves IGFBP-4 and IGFBP-5, resulting in release of bound IGF. Cleavage of IGFBP-4 is dramatically enhanced by the presence of IGF, whereas cleavage of IGFBP-5 is slightly inhibited by the presence of IGF.
Alternative names
Pappalysin-1, Insulin-like growth factor-dependent IGF-binding protein 4 protease, Pregnancy-associated plasma protein A, IGF-dependent IGFBP-4 protease, IGFBP-4ase, PAPP-A, PAPPA
Recommended products
Rabbit Recombinant Monoclonal PAPP A antibody. Carrier free. Suitable for IHC-P, IP, WB and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR12897
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab249813 is the carrier-free version of Anti-PAPP A antibody [EPR12897] ab174314.
Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-PAPP A antibody [EPR12897] - BSA and Azide free (ab249813), expandable thumbnail](https://content.abcam.com/products/images/papp-a-antibody-epr12897-bsa-and-azide-free-ab249813--western-blot-img85260.jpg "Western blot - Anti-PAPP A antibody [EPR12897] - BSA and Azide free (ab249813)")
Western blot - Anti-PAPP A antibody [EPR12897] - BSA and Azide free (ab249813)
This data was developed using Anti-PAPP A antibody [EPR12897] ab174314, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-PAPP A antibody [EPR12897] (Anti-PAPP A antibody [EPR12897] ab174314) at 1/1000 dilution
All lanes: Human placenta lysate at 10 µg
Predicted band size: 180 kDa
![Immunoprecipitation - Anti-PAPP A antibody [EPR12897] - BSA and Azide free (ab249813), expandable thumbnail](https://content.abcam.com/products/images/papp-a-antibody-epr12897-bsa-and-azide-free-ab249813--immunoprecipitation-img72511.jpg "Immunoprecipitation - Anti-PAPP A antibody [EPR12897] - BSA and Azide free (ab249813)")
Immunoprecipitation - Anti-PAPP A antibody [EPR12897] - BSA and Azide free (ab249813)
This data was developed using Anti-PAPP A antibody [EPR12897] ab174314, the same antibody clone in a different buffer formulation.Western blot analysis on immunoprecipitation pellet from Human placenta lysate using Anti-PAPP A antibody [EPR12897] ab174314 at a 1/10 dilution.
All lanes: Immunoprecipitation - Anti-PAPP A antibody [EPR12897] (Anti-PAPP A antibody [EPR12897] ab174314)
Predicted band size: 180 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAPP A antibody [EPR12897] - BSA and Azide free (ab249813), expandable thumbnail](https://content.abcam.com/products/images/papp-a-antibody-epr12897-bsa-and-azide-free-ab249813--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img56795.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAPP A antibody [EPR12897] - BSA and Azide free (ab249813)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAPP A antibody [EPR12897] - BSA and Azide free (ab249813)
This data was developed using Anti-PAPP A antibody [EPR12897] ab174314, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling PAPP A with Anti-PAPP A antibody [EPR12897] ab174314 at a 1/100 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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