Anti-PAPP A2 antibody [EPR25642-91] - BSA and Azide free

• IHC

Supplier Data

Immunohistochemistry - Anti-PAPP A2 antibody [EPR25642-91] - BSA and Azide free (AB307088)

This data was developed using ab307087, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded human liver tissue labeling PAPP A2 with ab307087 at 1/5000 dilution (0.097 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Negative control : No staining on human liver. The section was incubated with ab307087 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC

Supplier Data

Immunohistochemistry - Anti-PAPP A2 antibody [EPR25642-91] - BSA and Azide free (AB307088)

This data was developed using ab307087, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded human placenta tissue labeling PAPP A2 with ab307087 at 1/5000 dilution (0.097 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on human placenta. The section was incubated with ab307087 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-PAPP A2 antibody [EPR25642-91] - BSA and Azide free (AB307088)

This data was developed using ab307087, the same antibody clone in a different buffer formulation.

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human placenta tissue staining PAPP A2 with ab307087 at a 1/5000 dilution, ab218107 anti-TFAP2C used at 1/200 dilution and ab315802 anti-CD34 used at a 1/2000 dilution.

Panel A : anti-PAPP A2 (green; Opal™520), anti-TFAP2C (magenta; Opal™690) and anti-CD34 (gray; Opal™570) on human placenta.

Panel B : anti-PAPP A2 staining cytoplasm of trophoblast in human placenta.

Panel C : anti-TFAP2C staining nucleus of trophoblast in human placenta.

Panel D : anti-CD34 staining endothelium in human placenta.

Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab307087, ab218107 and ab315802 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• WB

Supplier Data

Western blot - Anti-PAPP A2 antibody [EPR25642-91] - BSA and Azide free (AB307088)

This data was developed using ab307087, the same antibody clone in a different buffer formulation. Buffer/dilution : 5% NFDM/TBST Exposure : 3.25 seconds The molecular weight observed is consistent with what has been described in the literature (PMID : 18805800)

All lanes:

Western blot - Anti-PAPP A2 antibody [EPR25642-91] (<a href='/en-us/products/primary-antibodies/papp-a2-antibody-epr25642-91-ab307087'>ab307087</a>) at 1/1000 dilution

Lane 1:

Untreated human placenta tissue lysate at 15 µg

Lane 2:

Human placenta tissue lysate treated with Protein Deglycosylation Mix II at 15 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 220 kDa,270 kDa,250 kDa

false

Exposure time: 3.25s

• WB

Supplier Data

Western blot - Anti-PAPP A2 antibody [EPR25642-91] - BSA and Azide free (AB307088)

This data was developed using ab307087, the same antibody clone in a different buffer formulation. Buffer/dilution concentration : 5% NFDM/TBST Exposure time : 3.25 seconds Negative control : human liver (PMID : 11597188) Low expression : human kidney (PMID : 11597188)

All lanes:

Western blot - Anti-PAPP A2 antibody [EPR25642-91] (<a href='/en-us/products/primary-antibodies/papp-a2-antibody-epr25642-91-ab307087'>ab307087</a>) at 1/1000 dilution

Lane 1:

Human placenta tissue lysate at 20 µg

Lane 2:

Human kidney tissue lysate at 20 µg

Lane 3:

Human liver tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 250 kDa,270 kDa

false

Exposure time: 3.25s