Rabbit Recombinant Monoclonal PAPP A2 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.
Images
![Western blot - Anti-PAPP A2 antibody [EPR25642-91] - BSA and Azide free (AB307088), expandable thumbnail](https://content.abcam.com/products/images/papp-a2-antibody-epr25642-91-bsa-and-azide-free-ab307088--western-blot-img405163.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PAPP A2 antibody [EPR25642-91] - BSA and Azide free (AB307088)")
![Western blot - Anti-PAPP A2 antibody [EPR25642-91] - BSA and Azide free (AB307088), expandable thumbnail](https://content.abcam.com/products/images/papp-a2-antibody-epr25642-91-bsa-and-azide-free-ab307088--western-blot-img405162.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PAPP A2 antibody [EPR25642-91] - BSA and Azide free (AB307088)")
![Immunohistochemistry - Anti-PAPP A2 antibody [EPR25642-91] - BSA and Azide free (AB307088), expandable thumbnail](https://content.abcam.com/products/images/papp-a2-antibody-epr25642-91-bsa-and-azide-free-ab307088--immunohistochemistry-img405161.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry - Anti-PAPP A2 antibody [EPR25642-91] - BSA and Azide free (AB307088)")
![Immunohistochemistry - Anti-PAPP A2 antibody [EPR25642-91] - BSA and Azide free (AB307088), expandable thumbnail](https://content.abcam.com/products/images/papp-a2-antibody-epr25642-91-bsa-and-azide-free-ab307088--immunohistochemistry-img405160.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry - Anti-PAPP A2 antibody [EPR25642-91] - BSA and Azide free (AB307088)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | IHC-P | WB | |
|---|---|---|---|
| Human | Not recommended | Tested | Tested |
| Mouse | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat, Mouse | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Metalloproteinase which specifically cleaves insulin-like growth factor binding protein (IGFBP)-5 at the '163-Ser-|-Lys-164' bond. Shows limited proteolysis toward IGFBP-3.
Alternative names
PLAC3, PAPPA2, Pappalysin-2, Pregnancy-associated plasma protein A2, Pregnancy-associated plasma protein E1, PAPP-A2, PAPP-E
Recommended products
Rabbit Recombinant Monoclonal PAPP A2 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR25642-91
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-PAPP A2 antibody [EPR25642-91] - BSA and Azide free (ab307088), expandable thumbnail](https://content.abcam.com/products/images/papp-a2-antibody-epr25642-91-bsa-and-azide-free-ab307088--western-blot-img405163.jpg "Western blot - Anti-PAPP A2 antibody [EPR25642-91] - BSA and Azide free (ab307088)")
Western blot - Anti-PAPP A2 antibody [EPR25642-91] - BSA and Azide free (ab307088)
This data was developed using Anti-PAPP A2 antibody [EPR25642-91] ab307087, the same antibody clone in a different buffer formulation.
Buffer/dilution: 5% NFDM/TBST
Exposure: 3.25 seconds
The molecular weight observed is consistent with what has been described in the literature (PMID: 18805800)All lanes: Western blot - Anti-PAPP A2 antibody [EPR25642-91] (Anti-PAPP A2 antibody [EPR25642-91] ab307087) at 1/1000 dilution
Lane 1: Untreated human placenta tissue lysate at 15 µg
Lane 2: Human placenta tissue lysate treated with Protein Deglycosylation Mix II at 15 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Observed band size: 220 kDa, 270 kDa, 250 kDa
Exposure time: 3.25s
![Western blot - Anti-PAPP A2 antibody [EPR25642-91] - BSA and Azide free (ab307088), expandable thumbnail](https://content.abcam.com/products/images/papp-a2-antibody-epr25642-91-bsa-and-azide-free-ab307088--western-blot-img405162.jpg "Western blot - Anti-PAPP A2 antibody [EPR25642-91] - BSA and Azide free (ab307088)")
Western blot - Anti-PAPP A2 antibody [EPR25642-91] - BSA and Azide free (ab307088)
This data was developed using Anti-PAPP A2 antibody [EPR25642-91] ab307087, the same antibody clone in a different buffer formulation.
Buffer/dilution concentration: 5% NFDM/TBST
Exposure time: 3.25 seconds
Negative control: human liver (PMID: 11597188)
Low expression: human kidney (PMID: 11597188)All lanes: Western blot - Anti-PAPP A2 antibody [EPR25642-91] (Anti-PAPP A2 antibody [EPR25642-91] ab307087) at 1/1000 dilution
Lane 1: Human placenta tissue lysate at 20 µg
Lane 2: Human kidney tissue lysate at 20 µg
Lane 3: Human liver tissue lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Observed band size: 250 kDa, 270 kDa
Exposure time: 3.25s
![Immunohistochemistry - Anti-PAPP A2 antibody [EPR25642-91] - BSA and Azide free (ab307088), expandable thumbnail](https://content.abcam.com/products/images/papp-a2-antibody-epr25642-91-bsa-and-azide-free-ab307088--immunohistochemistry-img405161.jpg "Immunohistochemistry - Anti-PAPP A2 antibody [EPR25642-91] - BSA and Azide free (ab307088)")
Immunohistochemistry - Anti-PAPP A2 antibody [EPR25642-91] - BSA and Azide free (ab307088)
This data was developed using Anti-PAPP A2 antibody [EPR25642-91] ab307087, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human placenta tissue labeling PAPP A2 with Anti-PAPP A2 antibody [EPR25642-91] ab307087 at 1/5000 dilution (0.097 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Positive staining on human placenta.
The section was incubated with Anti-PAPP A2 antibody [EPR25642-91] ab307087 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.![Immunohistochemistry - Anti-PAPP A2 antibody [EPR25642-91] - BSA and Azide free (ab307088), expandable thumbnail](https://content.abcam.com/products/images/papp-a2-antibody-epr25642-91-bsa-and-azide-free-ab307088--immunohistochemistry-img405160.jpg "Immunohistochemistry - Anti-PAPP A2 antibody [EPR25642-91] - BSA and Azide free (ab307088)")
Immunohistochemistry - Anti-PAPP A2 antibody [EPR25642-91] - BSA and Azide free (ab307088)
This data was developed using Anti-PAPP A2 antibody [EPR25642-91] ab307087, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human liver tissue labeling PAPP A2 with Anti-PAPP A2 antibody [EPR25642-91] ab307087 at 1/5000 dilution (0.097 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Negative control: No staining on human liver.
The section was incubated with Anti-PAPP A2 antibody [EPR25642-91] ab307087 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
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