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Rabbit Polyclonal PAPSS1 antibody. Suitable for WB, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1.

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Images

Western blot - Anti-PAPSS1 antibody (AB228622), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAPSS1 antibody (AB228622), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAPSS1 antibody (AB228622), expandable thumbnail

Key facts

Host species

Rabbit

Storage buffer

pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1. The exact immunogen used to generate this antibody is proprietary information. Database link O43252

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-P
Human
Tested
Tested
Mouse
Predicted
Predicted
Cow
Predicted
Predicted
Guinea pig
Predicted
Predicted
Rabbit
Predicted
Predicted
Xenopus laevis
Predicted
Predicted

Tested
Tested

Species

Human

Dilution info

1/500.00000 - 1/3000.00000

Notes

-

Predicted
Predicted

Species

Mouse, Rabbit, Guinea pig, Cow, Xenopus laevis

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100.00000 - 1/1000.00000

Notes

-

Predicted
Predicted

Species

Mouse, Rabbit, Guinea pig, Cow, Xenopus laevis

Dilution info

-

Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Bifunctional enzyme with both ATP sulfurylase and APS kinase activity, which mediates two steps in the sulfate activation pathway. The first step is the transfer of a sulfate group to ATP to yield adenosine 5'-phosphosulfate (APS), and the second step is the transfer of a phosphate group from ATP to APS yielding 3'-phosphoadenylylsulfate (PAPS: activated sulfate donor used by sulfotransferase). In mammals, PAPS is the sole source of sulfate; APS appears to be only an intermediate in the sulfate-activation pathway (PubMed:14747722, PubMed:9576487, PubMed:9648242, PubMed:9668121). Required for normal biosynthesis of sulfated L-selectin ligands in endothelial cells (PubMed:9576487).

Alternative names

Recommended products

Rabbit Polyclonal PAPSS1 antibody. Suitable for WB, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1.

Key facts

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1. The exact immunogen used to generate this antibody is proprietary information. Database link O43252
Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-PAPSS1 antibody (ab228622), expandable thumbnail

    Western blot - Anti-PAPSS1 antibody (ab228622)

    7.5% SDS PAGE gel.

    All lanes: Western blot - Anti-PAPSS1 antibody (ab228622) at 1/1000 dilution

    All lanes: MCF7 (human breast adenocarcinoma cell line) whole cell lysate at 30 µg

    Predicted band size: 71 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAPSS1 antibody (ab228622), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAPSS1 antibody (ab228622)

    Paraffin-embedded human colon carcinoma tissue stained for PAPSS1 using ab228622 at 1/500 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAPSS1 antibody (ab228622), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAPSS1 antibody (ab228622)

    Paraffin-embedded human gastric cancer tissue stained for PAPSS1 using ab228622 at 1/500 dilution in immunohistochemical analysis.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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