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AB155585

Anti-PAPSS2 antibody

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(1 Publication)

Rabbit Polyclonal PAPSS2 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human PAPSS2 aa 300 to C-terminus.

View Alternative Names

ATPSK2, PAPSS2, Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 2, PAPS synthase 2, PAPSS 2, Sulfurylase kinase 2, SK 2, SK2

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-PAPSS2 antibody (AB155585)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PAPSS2 antibody (AB155585)

Immunofluorescent analysis of paraformaldehyde-fixed A431 cells labeling PAPSS2 with ab155585 at 1/200 dilution. Lower image shows cells co-stained with Hoechst 33342.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAPSS2 antibody (AB155585)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAPSS2 antibody (AB155585)

Immunohistochemical analysis of paraffin-embedded Human H3347 xenograft tissue labeling PAPSS2 with ab155585 at 1/500 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAPSS2 antibody (AB155585)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAPSS2 antibody (AB155585)

Immunohistochemical analysis of paraffin-embedded rat colon tissue labelling PAPSS2 protein at cytoplasm with ab155585 at a 1/500 dilution.
Antigen retrieval : Citrate buffer, pH 6.0, 15 min

Western blot - Anti-PAPSS2 antibody (AB155585)
  • WB

Unknown

Western blot - Anti-PAPSS2 antibody (AB155585)

7.5% SDS PAGE

All lanes:

Western blot - Anti-PAPSS2 antibody (ab155585) at 1/1000 dilution

All lanes:

H1299 whole cell lysate at 30 µg

Predicted band size: 70 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Rat, Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human PAPSS2 aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O95340

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p> Retrieve antigens with 70% Formic acid for 10-30 minutes at room temperature before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Bifunctional enzyme with both ATP sulfurylase and APS kinase activity, which mediates two steps in the sulfate activation pathway. The first step is the transfer of a sulfate group to ATP to yield adenosine 5'-phosphosulfate (APS), and the second step is the transfer of a phosphate group from ATP to APS yielding 3'-phosphoadenylylsulfate/PAPS, the activated sulfate donor used by sulfotransferases (PubMed : 11773860, PubMed : 19474428, PubMed : 23824674, PubMed : 25594860). In mammals, PAPS is the sole source of sulfate while APS appears to only be an intermediate in the sulfate-activation pathway (PubMed : 11773860, PubMed : 19474428, PubMed : 23824674, PubMed : 25594860). Plays indirectly an important role in skeletogenesis during postnatal growth (PubMed : 9771708).
See full target information PAPSS2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 295:15988-16001 PubMed32913122

2020

GGPP depletion initiates metaflammation through disequilibrating CYB5R3-dependent eicosanoid metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Lisha Wei,Yan-Yan Zheng,Jie Sun,Pei Wang,Tao Tao,Yeqiong Li,Xin Chen,Yongjuan Sang,Danyang Chong,Wei Zhao,Yuwei Zhou,Ye Wang,Zhihui Jiang,Tiantian Qiu,Chao-Jun Li,Min-Sheng Zhu,Xuena Zhang
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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