Anti-PAR-3/PARD3 antibody

• WB

Supplier Data

Western blot - Anti-PAR-3/PARD3 antibody (AB191204)

all samples were prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-PAR-3/PARD3 antibody (ab191204) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

293T whole cell lysate at 50 µg

Lane 3:

Jurkat whole cell lysate at 50 µg

Lane 4:

mouse TCMK-1 whole cell lysate at 50 µg

Lane 5:

mouse NIH 3T3 whole cell lysate at 50 µg

Predicted band size: 151 kDa

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Exposure time: 3min

• WB

CiteAb

Western blot - Anti-PAR-3/PARD3 antibody (AB191204)

PAR-3/PARD3 western blot using anti-PAR-3/PARD3 antibody ab191204. Publication image and figure legend from Wang, Z. & Jin, J., 2019, Aging (Albany NY), PubMed 31308265.

ab191204 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab191204 please see the product overview.

SLCO4A1-AS1 regulated PARD3 expression by sponging miR-508-3p. (A) The diagram illustrated the predicted and mutant 3’-UTR of PARD3; (B) The targeted miRNA levels were measured by qRT-PCR in SW620 cells; (C) PARD3 expression in SW620 cells after transfection with specific miRNA mimics or inhibitor (miR-508-3p, miR-26a,miR-486-5p) detected by Western blot. (D) Enrichment of SLCO4A1-AS1 and miR-508-3p by using AGO2 antibody in SW620 cells by RIP assay. (E) miR-508-3p binding sites in SLCO4A1-AS1 3’-UTR region were predicted by TargetScan; (F, G) Luciferase activity of HEK293T and SW620 cells after co-transfection with PARD3-3’UTR-WT (or Mut) and miR-508-3p or SLCO4A1-AS-3’UTR-WT (or Mut)and miR-508-3p detected by dual-luciferase assays; (H, I) miR-508-3p expressions with up- or down-regulation of SLCO4A1-AS1 expression in CRC cells by qRT-PCR.

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