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AB111976

Anti-PAR1/Thrombin Receptor antibody

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(2 Publications)

Goat Polyclonal PAR1/Thrombin Receptor antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human F2R aa 1-50.

View Alternative Names

CF2R, PAR1, TR, F2R, Proteinase-activated receptor 1, PAR-1, Coagulation factor II receptor, Thrombin receptor

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAR1/Thrombin Receptor antibody (AB111976)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAR1/Thrombin Receptor antibody (AB111976)

ab111976, at 2.5μg/ml, staining PAR1/Thrombin Receptor in Formalin-fixed, Paraffin-embedded Human Liver tissue by Immunohistochemistry.

Western blot - Anti-PAR1/Thrombin Receptor antibody (AB111976)
  • WB

Unknown

Western blot - Anti-PAR1/Thrombin Receptor antibody (AB111976)

All lanes:

Western blot - Anti-PAR1/Thrombin Receptor antibody (ab111976) at 0.5 µg/mL

All lanes:

HeLa lysate (in RIPA buffer) at 35 µg

Predicted band size: 47 kDa

true

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human F2R aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

P25116

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "2.5 µg/mL", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 99% Tris buffered saline, 0.5% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

High affinity receptor that binds the activated thrombin, leading to calcium release from intracellular stores (PubMed : 1672265, PubMed : 8136362). The thrombin-activated receptor signaling pathway is mediated through PTX-insensitive G proteins, activation of phospholipase C resulting in the production of 1D-myo-inositol 1,4,5-trisphosphate (InsP3) which binds to InsP3 receptors causing calcium release from the stores (By similarity). In astrocytes, the calcium released into the cytosol allows the Ca(2+)-dependent release of L-glutamate into the synaptic cleft through BEST1, that targets the neuronal postsynaptic GRIN2A/NMDAR receptor resulting in the synaptic plasticity regulation (By similarity). May play a role in platelets activation and in vascular development (PubMed : 10079109). Mediates up-regulation of pro-inflammatory cytokines, such as MCP-1/CCL2 and IL6, triggered by coagulation factor Xa (F10) in cardiac fibroblasts and umbilical vein endothelial cells (PubMed : 30568593, PubMed : 34831181).
See full target information F2R
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

British journal of pharmacology 175:3656-3668 PubMed29959891

2018

Thrombin modifies growth, proliferation and apoptosis of human colon organoids: a protease-activated receptor 1- and protease-activated receptor 4-dependent mechanism.

Applications

Unspecified application

Species

Unspecified reactive species

Morgane Sébert,Alexandre Denadai-Souza,Muriel Quaranta,Claire Racaud-Sultan,Sophie Chabot,Philippe Lluel,Nicolas Monjotin,Laurent Alric,Guillaume Portier,Sylvain Kirzin,Delphine Bonnet,Audrey Ferrand,Nathalie Vergnolle

Pain 159:1257-1267 PubMed29554016

2018

Protease-activated receptor 1 is implicated in irritable bowel syndrome mediators-induced signaling to thoracic human sensory neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Cleo Desormeaux,Tereza Bautzova,Sonia Garcia-Caraballo,Corinne Rolland,Maria Raffaella Barbaro,Stuart M Brierley,Giovanni Barbara,Nathalie Vergnolle,Nicolas Cenac
View all publications
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Product promise

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