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Rabbit Recombinant Monoclonal PAR1/Thrombin Receptor antibody. Suitable for mIHC, IHC-P, WB and reacts with Mouse samples.

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Images

Multiplex immunohistochemistry - Anti-PAR1/Thrombin Receptor antibody [EPR28398-8] (AB322457), expandable thumbnail
  • Western blot - Anti-PAR1/Thrombin Receptor antibody [EPR28398-8] (AB322457), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAR1/Thrombin Receptor antibody [EPR28398-8] (AB322457), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAR1/Thrombin Receptor antibody [EPR28398-8] (AB322457), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAR1/Thrombin Receptor antibody [EPR28398-8] (AB322457), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
mIHCIHC-PWBICC/IF
Mouse
Tested
Tested
Tested
Not recommended

Tested
Tested

Species
Mouse
Dilution info
1/500
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/500
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

-

Target data

Function

High affinity receptor that binds the activated thrombin, leading to calcium release from intracellular stores. The thrombin-activated receptor signaling pathway is mediated through PTX-insensitive G proteins, activation of phospholipase C resulting in the production of 1D-myo-inositol 1,4,5-trisphosphate (InsP3) which binds to InsP3 receptors causing calcium release from the stores (By similarity). In astrocytes, the calcium released into the cytosol allows the Ca(2+)-dependent release of L-glutamate into the synaptic cleft through BEST1, that targets the neuronal postsynaptic GRIN2A/NMDAR receptor resulting in the synaptic plasticity regulation (PubMed:25645137). May play a role in platelets activation and in vascular development (By similarity). Mediates up-regulation of pro-inflammatory cytokines, such as MCP-1/CCL2 and IL6, triggered by coagulation factor Xa (F10) in cardiac fibroblasts and umbilical vein endothelial cells (By similarity).

Alternative names

Cf2r, Par1, Proteinase-activated receptor 1, PAR-1, Thrombin receptor

Rabbit Recombinant Monoclonal PAR1/Thrombin Receptor antibody. Suitable for mIHC, IHC-P, WB and reacts with Mouse samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR28398-8
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Multiplex immunohistochemistry - Anti-PAR1/Thrombin Receptor antibody [EPR28398-8] (ab322457), expandable thumbnail

    Multiplex immunohistochemistry - Anti-PAR1/Thrombin Receptor antibody [EPR28398-8] (ab322457)

    PAR1/Thrombin Receptor Multiplex immunohistochemistry staining of Mouse liver using rabbit Anti-PAR1/Thrombin Receptor antibody

    Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse liver tissue staining ALDH1L1 with Anti-ALDH1L1 antibody [EPR25443-103] - Astrocyte Marker ab307696 at a 1/5000 dilution, ab322457 anti-PAR1 used at 1/500 dilution and Anti-ABCB11/BSEP antibody [EPR28773-83] ab315474 anti-ABCB11 used at a 1/2000 dilution.

    Panel A: merged staining of anti-ALDH1L1 (green; Opal™520), anti-PAR1 (magenta; Opal™690) and anti-ABCB11 (gray; Opal™570) on mouse liver.

    Panel B: anti-ALDH1L1 staining hepatocytes in mouse liver.

    Panel C: anti-PAR1 staining endothelium in mouse liver.

    Panel D: anti-ABCB11 staining bile canaliculi in mouse liver.

    Nuclear DNA was labeled with DAPI (shown in blue).

    The section was incubated in three rounds of staining: in the order of Anti-ALDH1L1 antibody [EPR25443-103] - Astrocyte Marker ab307696, ab322457 and Anti-ABCB11/BSEP antibody [EPR28773-83] ab315474 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Western blot - Anti-PAR1/Thrombin Receptor antibody [EPR28398-8] (ab322457), expandable thumbnail

    Western blot - Anti-PAR1/Thrombin Receptor antibody [EPR28398-8] (ab322457)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Low expression: RAW 264.7, HL-1.

    PAR1 contains different glycosylation sites, the multiple bands may be due to glycosylation (PMID: 20368337).

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-PAR1/Thrombin Receptor antibody [EPR28398-8] (ab322457) at 1/1000 dilution

    Lane 1: Mouse dorsal galion tissue lysate at 20 µg

    Lane 2: Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 20 µg

    Lane 3: RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg

    Lane 4: HL-1 (mouse atrial muscle cell) whole cell lysate at 20 µg

    Lane 5: C8-D1A (mouse cerebellum astrocyte) whole cell lysate at 20 µg

    Lane 6: SW10 (mouse neuronal schwann cell) whole cell lysate at 20 µg

    Lane 7: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 48-75 kDa, 36 kDa

    Exposure time: 136s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAR1/Thrombin Receptor antibody [EPR28398-8] (ab322457), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAR1/Thrombin Receptor antibody [EPR28398-8] (ab322457)

    Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling PAR1/Thrombin Receptor with ab322457 at 1/500 (1.054 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on mouse liver.

    The section was incubated with ab322457 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAR1/Thrombin Receptor antibody [EPR28398-8] (ab322457), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAR1/Thrombin Receptor antibody [EPR28398-8] (ab322457)

    Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling PAR1/Thrombin Receptor with ab322457 at 1/500 (1.054 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on mouse spleen.

    The section was incubated with ab322457 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAR1/Thrombin Receptor antibody [EPR28398-8] (ab322457), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAR1/Thrombin Receptor antibody [EPR28398-8] (ab322457)

    Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labeling PAR1/Thrombin Receptor with ab322457 at 1/500 (1.054 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on mouse lung (PMID: 12548614).

    The section was incubated with ab322457 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAR1/Thrombin Receptor antibody [EPR28398-8] (ab322457), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAR1/Thrombin Receptor antibody [EPR28398-8] (ab322457)

    Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling PAR1/Thrombin Receptor with ab322457 at 1/500 (1.054 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on mouse kidney.

    The section was incubated with ab322457 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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